PMID- 31922247
OWN - NLM
STAT- MEDLINE
DCOM- 20200821
LR  - 20211112
IS  - 1791-2431 (Electronic)
IS  - 1021-335X (Linking)
VI  - 43
IP  - 2
DP  - 2020 Feb
TI  - Combination of AAV‑mediated NUPR1 knockdown and trifluoperazine induces premature 
      senescence in human lung adenocarcinoma A549 cells in nude mice.
PG  - 681-688
LID - 10.3892/or.2020.7455 [doi]
AB  - Nuclear protein 1 (NUPR1)/p8, a transcriptional regulator, has the ability to 
      facilitate lung cancer cell survival. Adeno‑associated virus (AAV)‑based vectors 
      are efficient vehicles for gene transfer and expression. In this study, an 
      AAV‑mediated NUPR1 shRNA vector was constructed that effectively inhibited the 
      expression of NUPR1 in a tumor xenograft model derived from lung adenocarcinoma 
      A549 cells. Trifluoperazine (TFP), which is an antipsychotic drug, has the 
      ability to bind to NUPR1 and mimic NUPR1 deficiency in cancer cells. It was also 
      found that the combination of TFP and AAV‑mediated NUPR1 shRNA delivery led to 
      significant tumor growth inhibition in nude mice bearing human lung cancer 
      xenografts. Moreover, AAV‑mediated NUPR1 shRNA therapy induced premature 
      senescence in vitro and in vivo. Collectively, the findings of this study suggest 
      a putative role for the combination of AAV‑NUPR1 shRNA and TFP in lung cancer 
      therapy.
FAU - Li, Yanzhe
AU  - Li Y
AD  - Tianjin Key Laboratory of Medical Epigenetics, Key Laboratory of Immune 
      Microenvironment and Disease (Ministry of Education), Department of Immunology, 
      Biochemistry and Molecular Biology, Tianjin Medical University, Tianjin 300070, 
      P.R. China.
FAU - Yin, Yueyuan
AU  - Yin Y
AD  - Tianjin Key Laboratory of Medical Epigenetics, Key Laboratory of Immune 
      Microenvironment and Disease (Ministry of Education), Department of Immunology, 
      Biochemistry and Molecular Biology, Tianjin Medical University, Tianjin 300070, 
      P.R. China.
FAU - Ma, Jinyi
AU  - Ma J
AD  - Tianjin Key Laboratory of Medical Epigenetics, Key Laboratory of Immune 
      Microenvironment and Disease (Ministry of Education), Department of Immunology, 
      Biochemistry and Molecular Biology, Tianjin Medical University, Tianjin 300070, 
      P.R. China.
FAU - Sun, Yanan
AU  - Sun Y
AD  - Tianjin Key Laboratory of Medical Epigenetics, Key Laboratory of Immune 
      Microenvironment and Disease (Ministry of Education), Department of Immunology, 
      Biochemistry and Molecular Biology, Tianjin Medical University, Tianjin 300070, 
      P.R. China.
FAU - Zhou, Ruimin
AU  - Zhou R
AD  - Tianjin Key Laboratory of Medical Epigenetics, Key Laboratory of Immune 
      Microenvironment and Disease (Ministry of Education), Department of Immunology, 
      Biochemistry and Molecular Biology, Tianjin Medical University, Tianjin 300070, 
      P.R. China.
FAU - Cui, Bo
AU  - Cui B
AD  - Department of Traditional Chinese Medicine, Yangzhou East Hospital, Yangzhou, 
      Jiangsu 225002, P.R. China.
FAU - Zhang, Yuxuan
AU  - Zhang Y
AD  - Center for Translational Systems Biology and Neuroscience and Key Laboratory of 
      Integrative Biomedicine for Brain Diseases, Nanjing University of Chinese 
      Medicine, Nanjing, Jiangsu 210023, P.R. China.
FAU - Yang, Jie
AU  - Yang J
AD  - Tianjin Key Laboratory of Medical Epigenetics, Key Laboratory of Immune 
      Microenvironment and Disease (Ministry of Education), Department of Immunology, 
      Biochemistry and Molecular Biology, Tianjin Medical University, Tianjin 300070, 
      P.R. China.
FAU - Yan, Xiaojie
AU  - Yan X
AD  - Tianjin Key Laboratory of Medical Epigenetics, Key Laboratory of Immune 
      Microenvironment and Disease (Ministry of Education), Department of Immunology, 
      Biochemistry and Molecular Biology, Tianjin Medical University, Tianjin 300070, 
      P.R. China.
FAU - Liu, Zhe
AU  - Liu Z
AD  - Tianjin Key Laboratory of Medical Epigenetics, Key Laboratory of Immune 
      Microenvironment and Disease (Ministry of Education), Department of Immunology, 
      Biochemistry and Molecular Biology, Tianjin Medical University, Tianjin 300070, 
      P.R. China.
FAU - Ma, Zhenyi
AU  - Ma Z
AD  - Tianjin Key Laboratory of Medical Epigenetics, Key Laboratory of Immune 
      Microenvironment and Disease (Ministry of Education), Department of Immunology, 
      Biochemistry and Molecular Biology, Tianjin Medical University, Tianjin 300070, 
      P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20200108
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
RN  - 0 (NUPR1 protein, human)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (RNA, Small Interfering)
RN  - 214IZI85K3 (Trifluoperazine)
SB  - IM
MH  - A549 Cells
MH  - Adenocarcinoma of Lung/*drug therapy/genetics/pathology
MH  - Animals
MH  - Basic Helix-Loop-Helix Transcription Factors/*antagonists & inhibitors/genetics
MH  - Cell Survival/drug effects
MH  - Dependovirus/genetics
MH  - Drug Synergism
MH  - Gene Knockdown Techniques
MH  - Genetic Vectors/administration & dosage
MH  - Humans
MH  - Lung Neoplasms/drug therapy/*genetics/pathology
MH  - Mice
MH  - Mice, Nude
MH  - Neoplasm Proteins/*antagonists & inhibitors/genetics
MH  - RNA, Small Interfering/*administration & dosage/pharmacology
MH  - Trifluoperazine/*administration & dosage/pharmacology
MH  - Xenograft Model Antitumor Assays
OTO - NOTNLM
OT  - NUPR1/p8
OT  - TFP
OT  - autophagy
OT  - lung cancer
OT  - premature senescence
EDAT- 2020/01/11 06:00
MHDA- 2020/08/22 06:00
CRDT- 2020/01/11 06:00
PHST- 2019/07/24 00:00 [received]
PHST- 2019/12/02 00:00 [accepted]
PHST- 2020/01/11 06:00 [pubmed]
PHST- 2020/08/22 06:00 [medline]
PHST- 2020/01/11 06:00 [entrez]
AID - 10.3892/or.2020.7455 [doi]
PST - ppublish
SO  - Oncol Rep. 2020 Feb;43(2):681-688. doi: 10.3892/or.2020.7455. Epub 2020 Jan 8.