PMID- 31923490
OWN - NLM
STAT- MEDLINE
DCOM- 20201123
LR  - 20201123
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 146
DP  - 2020 Mar 1
TI  - Polysaccharides from loquat (Eriobotrya japonica) leaves: Impacts of extraction 
      methods on their physicochemical characteristics and biological activities.
PG  - 508-517
LID - S0141-8130(19)40502-3 [pii]
LID - 10.1016/j.ijbiomac.2019.12.273 [doi]
AB  - Impacts of hot water extraction (HWE), pressurized water extraction (PWE), 
      high-speed shearing homogenization extraction, microwave assisted extraction 
      (MAE), ultrasound assisted extraction (UAE), ultrasound assisted enzymatic 
      extraction, and ultrasound-microwave assisted extraction (UMAE) on 
      physicochemical characteristics and bioactivities of polysaccharides from loquat 
      (Eriobotrya japonica) leaves (LLPs) were investigated. Results showed that the 
      degrees of esterification, contents of phenolics and uronic acids, constituent 
      monosaccharides, apparent viscosities, and molecular weights of LLPs varied by 
      different extraction methods. Bioactivities of LLPs were also significantly 
      affected by different extraction methods. The high molecular weight and high 
      degree of esterification of LLP-W and LLP-P extracted by HWE and PWE, 
      respectively, might contribute to their strong binding capacities. The strong 
      antioxidant activities and inhibitory effects on alpha-amylase and alpha-glucosidase were 
      found in LLP-M and LLP-U extracted by MAE and UAE, respectively, which might be 
      attributed to their contents of uronic acids, contents of total phenolics, and 
      molecular weights. The low molecular weights and viscosities of LLP-U and LLP-UM 
      extracted by UMAE might contribute to their strong prebiotic effects. These 
      findings could provide scientific foundations for selecting appropriate 
      extraction methods to obtain LLPs with desired bioactivities for applications in 
      the pharmaceutical and functional food industries.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Fu, Yuan
AU  - Fu Y
AD  - Institute of Food Processing and Safety, College of Food Science, Sichuan 
      Agricultural University, Ya'an 625014, Sichuan, China.
FAU - Li, Fen
AU  - Li F
AD  - Institute of Food Processing and Safety, College of Food Science, Sichuan 
      Agricultural University, Ya'an 625014, Sichuan, China.
FAU - Ding, Ye
AU  - Ding Y
AD  - Institute of Food Processing and Safety, College of Food Science, Sichuan 
      Agricultural University, Ya'an 625014, Sichuan, China.
FAU - Li, Hua-Yu
AU  - Li HY
AD  - Institute of Food Processing and Safety, College of Food Science, Sichuan 
      Agricultural University, Ya'an 625014, Sichuan, China.
FAU - Xiang, Xian-Rong
AU  - Xiang XR
AD  - Institute of Food Processing and Safety, College of Food Science, Sichuan 
      Agricultural University, Ya'an 625014, Sichuan, China.
FAU - Ye, Qiong
AU  - Ye Q
AD  - Institute of Food Processing and Safety, College of Food Science, Sichuan 
      Agricultural University, Ya'an 625014, Sichuan, China.
FAU - Zhang, Jian
AU  - Zhang J
AD  - Institute of Food Processing and Safety, College of Food Science, Sichuan 
      Agricultural University, Ya'an 625014, Sichuan, China.
FAU - Zhao, Li
AU  - Zhao L
AD  - Institute of Food Processing and Safety, College of Food Science, Sichuan 
      Agricultural University, Ya'an 625014, Sichuan, China.
FAU - Qin, Wen
AU  - Qin W
AD  - Institute of Food Processing and Safety, College of Food Science, Sichuan 
      Agricultural University, Ya'an 625014, Sichuan, China.
FAU - Gan, Ren-You
AU  - Gan RY
AD  - Institute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu 
      610213, China. Electronic address: ganrenyou@caas.cn.
FAU - Wu, Ding-Tao
AU  - Wu DT
AD  - Institute of Food Processing and Safety, College of Food Science, Sichuan 
      Agricultural University, Ya'an 625014, Sichuan, China. Electronic address: 
      DT_Wu@sicau.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20200107
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Antioxidants)
RN  - 0 (Benzothiazoles)
RN  - 0 (Biphenyl Compounds)
RN  - 0 (Glycoside Hydrolase Inhibitors)
RN  - 0 (Monosaccharides)
RN  - 0 (Picrates)
RN  - 0 (Polysaccharides)
RN  - 0 (Sulfonic Acids)
RN  - 28752-68-3 (2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl)
RN  - EC 3.2.1.1 (alpha-Amylases)
RN  - EC 3.2.1.20 (alpha-Glucosidases)
SB  - IM
MH  - Antioxidants/pharmacology
MH  - Benzothiazoles/chemistry
MH  - Biphenyl Compounds/chemistry
MH  - *Chemical Phenomena
MH  - Cholesterol/metabolism
MH  - Eriobotrya/*chemistry
MH  - Esterification
MH  - Glycoside Hydrolase Inhibitors/pharmacology
MH  - Molecular Weight
MH  - Monosaccharides/analysis
MH  - Nitric Oxide/metabolism
MH  - Picrates/chemistry
MH  - Plant Leaves/*chemistry
MH  - Polysaccharides/*chemistry/*isolation & purification
MH  - Rheology
MH  - Spectroscopy, Fourier Transform Infrared
MH  - Sulfonic Acids/chemistry
MH  - Viscosity
MH  - alpha-Amylases/antagonists & inhibitors/metabolism
MH  - alpha-Glucosidases/metabolism
OTO - NOTNLM
OT  - Bioactivity
OT  - Chemical structure
OT  - Extraction method
OT  - Loquat
OT  - Polysaccharide
COIS- Declaration of competing interest The authors declare that there are no conflicts 
      of interest.
EDAT- 2020/01/11 06:00
MHDA- 2020/11/24 06:00
CRDT- 2020/01/11 06:00
PHST- 2019/12/20 00:00 [received]
PHST- 2019/12/27 00:00 [revised]
PHST- 2019/12/31 00:00 [accepted]
PHST- 2020/01/11 06:00 [pubmed]
PHST- 2020/11/24 06:00 [medline]
PHST- 2020/01/11 06:00 [entrez]
AID - S0141-8130(19)40502-3 [pii]
AID - 10.1016/j.ijbiomac.2019.12.273 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2020 Mar 1;146:508-517. doi: 10.1016/j.ijbiomac.2019.12.273. 
      Epub 2020 Jan 7.