PMID- 31924121
OWN - NLM
STAT- MEDLINE
DCOM- 20201125
LR  - 20210422
IS  - 1551-4005 (Electronic)
IS  - 1538-4101 (Print)
IS  - 1551-4005 (Linking)
VI  - 19
IP  - 3
DP  - 2020 Feb
TI  - Human umbilical cord mesenchymal stem cells-derived exosomes transfers 
      microRNA-19a to protect cardiomyocytes from acute myocardial infarction by 
      targeting SOX6.
PG  - 339-353
LID - 10.1080/15384101.2019.1711305 [doi]
AB  - Exosomes secreted by human umbilical cord mesenchymal stem cells (hucMSCs) 
      protect cardiomyocytes from anoxia-reoxygenation injury. But the mechanism of 
      hucMSC-exo-microRNA (miR)-19a in acute myocardial infarction (AMI) remains 
      unclear. For this study, cardiac function related indicators, inflammatory 
      factors and markers of myocardial injury, cardiomyocyte injury, infarct size, and 
      apoptosis were detected in vivo experiments. The gain-and loss-of function was 
      performed to evaluate the effects of hucMSC-exo with down/upregulated miR-19a on 
      AMI rats and hypoxic H9C2 cells. Western blot analysis was used to detect levels 
      of AKT/JNK3/caspase-3 axis-related proteins. Consequently, hucMSC-exo alleviated 
      AMI and inhibited cardiomyocyte apoptosis. miR-19a was downregulated in AMI 
      tissues and cells, and increased after hucMSC-exo treatment. miR-19a knockdown in 
      hucMSC-exo impaired the protective role of hucMSC-exo alone in the AMI damage. 
      SOX6 is a target gene of miR-19a and its inhibition lightened hypoxic damage of 
      H9C2 cells. SOX6 knockdown together with miR-19a inhibition in hucMSC-exo 
      activated AKT and inhibited JNK3/caspase-3 axis. Taken together, hucMSC-exo 
      protected cardiomyocytes from AMI injury by transferring miR-19a, targeting SOX6, 
      activating AKT, and inhibiting JNK3/caspase-3 activation. This study may provide 
      new understanding for AMI treatment.
FAU - Huang, Lin
AU  - Huang L
AD  - Department of Cardiovascular Medicine, The Second Affiliated Hospital of Nanchang 
      University, Nanchang, Jiangxi, P.R. China.
FAU - Yang, Lu
AU  - Yang L
AD  - Department of Cardiovascular Medicine, The Second Affiliated Hospital of Nanchang 
      University, Nanchang, Jiangxi, P.R. China.
FAU - Ding, Yin
AU  - Ding Y
AD  - Department of Cardiovascular Medicine, The Second Affiliated Hospital of Nanchang 
      University, Nanchang, Jiangxi, P.R. China.
FAU - Jiang, Xinghua
AU  - Jiang X
AD  - Department of Cardiovascular Medicine, The Second Affiliated Hospital of Nanchang 
      University, Nanchang, Jiangxi, P.R. China.
FAU - Xia, Zhen
AU  - Xia Z
AD  - Department of Cardiovascular Medicine, The Second Affiliated Hospital of Nanchang 
      University, Nanchang, Jiangxi, P.R. China.
FAU - You, Zhigang
AU  - You Z
AD  - Department of Cardiovascular Medicine, The Second Affiliated Hospital of Nanchang 
      University, Nanchang, Jiangxi, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200110
PL  - United States
TA  - Cell Cycle
JT  - Cell cycle (Georgetown, Tex.)
JID - 101137841
RN  - 0 (Cytokines)
RN  - 0 (MIRN19 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Protective Agents)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (SOX6 protein, human)
RN  - 0 (SOXD Transcription Factors)
RN  - EC 2.7.1.- (Mitogen-Activated Protein Kinase 10)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 3.4.22.- (CASP3 protein, human)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Animals
MH  - Apoptosis/genetics
MH  - Caspase 3/metabolism
MH  - Cell Hypoxia
MH  - Cell Line
MH  - Coculture Techniques
MH  - Cytokines/metabolism
MH  - Exosomes/genetics/*metabolism/ultrastructure
MH  - Humans
MH  - Male
MH  - Mesenchymal Stem Cells/cytology/*metabolism
MH  - MicroRNAs/genetics/*metabolism
MH  - Mitogen-Activated Protein Kinase 10/metabolism
MH  - Myocardial Infarction/genetics/*metabolism/therapy
MH  - Myocytes, Cardiac/*metabolism/pathology
MH  - Protective Agents/pharmacology
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reactive Oxygen Species/metabolism
MH  - SOXD Transcription Factors/genetics/*metabolism
MH  - Umbilical Cord/cytology/*metabolism
PMC - PMC7028160
OTO - NOTNLM
OT  - AKT/JNK3/caspase-3 axis
OT  - Acute myocardial infarction
OT  - Exosomes
OT  - Human umbilical cord mesenchymal stem cells
OT  - SOX6
OT  - microRNA-19a
EDAT- 2020/01/12 06:00
MHDA- 2020/11/26 06:00
PMCR- 2021/01/10
CRDT- 2020/01/12 06:00
PHST- 2020/01/12 06:00 [pubmed]
PHST- 2020/11/26 06:00 [medline]
PHST- 2020/01/12 06:00 [entrez]
PHST- 2021/01/10 00:00 [pmc-release]
AID - 1711305 [pii]
AID - 10.1080/15384101.2019.1711305 [doi]
PST - ppublish
SO  - Cell Cycle. 2020 Feb;19(3):339-353. doi: 10.1080/15384101.2019.1711305. Epub 2020 
      Jan 10.