PMID- 31924278
OWN - NLM
STAT- MEDLINE
DCOM- 20200727
LR  - 20231115
IS  - 1297-9716 (Electronic)
IS  - 0928-4249 (Print)
IS  - 0928-4249 (Linking)
VI  - 51
IP  - 1
DP  - 2020 Jan 10
TI  - Precision cut lung slices: a novel versatile tool to examine host-pathogen 
      interaction in the chicken lung.
PG  - 2
LID - 10.1186/s13567-019-0733-0 [doi]
LID - 2
AB  - The avian respiratory tract is a common entry route for many pathogens and an 
      important delivery route for vaccination in the poultry industry. Immune 
      responses in the avian lung have mostly been studied in vivo due to the lack of 
      robust, relevant in vitro and ex vivo models mimicking the microenvironment. 
      Precision-cut lung slices (PCLS) have the major advantages of maintaining the 
      3-dimensional architecture of the lung and includes heterogeneous cell 
      populations. PCLS have been obtained from a number of mammalian species and from 
      chicken embryos. However, as the embryonic lung is physiologically 
      undifferentiated and immunologically immature, it is less suitable to examine 
      complex host-pathogen interactions including antimicrobial responses. Here we 
      prepared PCLS from immunologically mature chicken lungs, tested different culture 
      conditions, and found that serum supplementation has a detrimental effect on the 
      quality of PCLS. Viable cells in PCLS remained present for >/= 40 days, as 
      determined by viability assays and sustained motility of fluorescent mononuclear 
      phagocytic cells. The PCLS were responsive to lipopolysaccharide stimulation, 
      which induced the release of nitric oxide, IL-1beta, type I interferons and IL-10. 
      Mononuclear phagocytes within the tissue maintained phagocytic activity, with 
      live cell imaging capturing interactions with latex beads and an avian pathogenic 
      Escherichia coli strain. Finally, the PCLS were also shown to be permissive to 
      infection with low pathogenic avian influenza viruses. Taken together, 
      immunologically mature chicken PCLS provide a suitable model to simulate live 
      organ responsiveness and cell dynamics, which can be readily exploited to examine 
      host-pathogen interactions and inflammatory responses.
FAU - Bryson, Karen Jane
AU  - Bryson KJ
AUID- ORCID: 0000-0002-5242-5583
AD  - Division of Infection and Immunity, The Roslin Institute, Royal (Dick) School of 
      Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian, Edinburgh, 
      Scotland, EH25 9RG, UK. karen.bryson@roslin.ed.ac.uk.
FAU - Garrido, Damien
AU  - Garrido D
AD  - INRAE, Universite de Tours, UMR ISP, Centre Val de Loire, 37380, Nouzilly, 
      France.
FAU - Esposito, Marco
AU  - Esposito M
AD  - Division of Developmental Biology, The Roslin Institute, Royal (Dick) School of 
      Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian, Edinburgh, 
      Scotland, EH25 9RG, UK.
FAU - McLachlan, Gerry
AU  - McLachlan G
AD  - Division of Developmental Biology, The Roslin Institute, Royal (Dick) School of 
      Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian, Edinburgh, 
      Scotland, EH25 9RG, UK.
FAU - Digard, Paul
AU  - Digard P
AD  - Division of Infection and Immunity, The Roslin Institute, Royal (Dick) School of 
      Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian, Edinburgh, 
      Scotland, EH25 9RG, UK.
FAU - Schouler, Catherine
AU  - Schouler C
AD  - INRAE, Universite de Tours, UMR ISP, Centre Val de Loire, 37380, Nouzilly, 
      France.
FAU - Guabiraba, Rodrigo
AU  - Guabiraba R
AD  - INRAE, Universite de Tours, UMR ISP, Centre Val de Loire, 37380, Nouzilly, 
      France. rodrigo.guabiraba-brito@inra.fr.
FAU - Trapp, Sascha
AU  - Trapp S
AD  - INRAE, Universite de Tours, UMR ISP, Centre Val de Loire, 37380, Nouzilly, 
      France.
FAU - Vervelde, Lonneke
AU  - Vervelde L
AD  - Division of Infection and Immunity, The Roslin Institute, Royal (Dick) School of 
      Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian, Edinburgh, 
      Scotland, EH25 9RG, UK.
LA  - eng
GR  - BB/J004219/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
GR  - 2015-67015-23093/BB_/Biotechnology and Biological Sciences Research 
      Council/United Kingdom
GR  - BB/M028208/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
GR  - BB/M028305/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
GR  - BBS/E/D/20002172/BB_/Biotechnology and Biological Sciences Research 
      Council/United Kingdom
PT  - Journal Article
PT  - Validation Study
DEP - 20200110
PL  - England
TA  - Vet Res
JT  - Veterinary research
JID - 9309551
RN  - 0 (Lipopolysaccharides)
SB  - IM
MH  - Animals
MH  - *Chickens/immunology
MH  - Host-Pathogen Interactions/*immunology
MH  - Lipopolysaccharides/metabolism
MH  - Lung/*immunology/microbiology/parasitology
MH  - Poultry Diseases/*immunology/microbiology/parasitology
MH  - Veterinary Medicine/*methods
PMC - PMC6954617
COIS- The authors declare that they have no competing interests.
EDAT- 2020/01/12 06:00
MHDA- 2020/07/28 06:00
PMCR- 2020/01/10
CRDT- 2020/01/12 06:00
PHST- 2019/08/13 00:00 [received]
PHST- 2019/12/12 00:00 [accepted]
PHST- 2020/01/12 06:00 [entrez]
PHST- 2020/01/12 06:00 [pubmed]
PHST- 2020/07/28 06:00 [medline]
PHST- 2020/01/10 00:00 [pmc-release]
AID - 10.1186/s13567-019-0733-0 [pii]
AID - 733 [pii]
AID - 10.1186/s13567-019-0733-0 [doi]
PST - epublish
SO  - Vet Res. 2020 Jan 10;51(1):2. doi: 10.1186/s13567-019-0733-0.