PMID- 31925827
OWN - NLM
STAT- MEDLINE
DCOM- 20210812
LR  - 20210812
IS  - 1365-2222 (Electronic)
IS  - 0954-7894 (Linking)
VI  - 50
IP  - 5
DP  - 2020 May
TI  - Neuronal branching of sensory neurons is associated with BDNF-positive 
      eosinophils in atopic dermatitis.
PG  - 577-584
LID - 10.1111/cea.13560 [doi]
AB  - BACKGROUND: Pruritus is a major symptom of atopic dermatitis (AD) and is 
      transmitted by a subpopulation of non-myelinated C-type free nerve endings in the 
      epidermis and upper dermis. Stimulation of these nerve terminals is affected by 
      histamine, neurotrophins and physical factors. Eosinophils of patients with AD 
      are a source of neurotrophins, including brain-derived neurotrophic factor 
      (BDNF), levels of which correlate with disease severity. OBJECTIVE: The purpose 
      of this study was to determine the anatomical localization of eosinophils in the 
      skin of patients with AD with regard to peripheral nerves and to investigate 
      whether eosinophils induce sprouting and neurite outgrowth in murine sensory 
      neurons. METHODS: Cryosections of skin derived from AD and control (NA) patients 
      were subjected to immunofluorescence analysis with markers for eosinophils, BDNF 
      and neuronal cells. Stimulated eosinophil supernatants were used for the 
      treatment of cultured peripheral mouse dorsal root ganglia (DRG) neurons followed 
      by morphometric analysis. RESULTS: Dermal axon density and the proximity of 
      eosinophils to nerve fibres were significantly higher in AD patients vs NA. Both 
      neuronal projections and eosinophils expressed BDNF. Furthermore, activated 
      eosinophil supernatants induced BDNF-dependent mouse DRG neuron branching. 
      CONCLUSIONS AND CLINICAL RELEVANCE: Our results indicate that BDNF-positive 
      eosinophils are also localized in close proximity with nerve fibres in AD, 
      suggesting a functional relationship between BDNF-expressing eosinophils and 
      neuronal projections. These observations suggest that eosinophils may have 
      considerable impact on pruritus by supporting sensory nerve branching.
CI  - (c) 2020 The Authors. Clinical & Experimental Allergy published by John Wiley & 
      Sons Ltd.
FAU - Guseva, Daria
AU  - Guseva D
AD  - Department of Cellular Neurophysiology, Hannover Medical School, Hannover, 
      Germany.
AD  - Department of Nutritional Medicine, University of Hohenheim, Stuttgart, Germany.
FAU - Rudrich, Urda
AU  - Rudrich U
AD  - Department of Dermatology and Allergy, Hannover Medical School, Hannover, 
      Germany.
FAU - Kotnik, Nika
AU  - Kotnik N
AD  - Division of Experimental Allergology and Immunodermatology, University of 
      Oldenburg, Oldenburg, Germany.
FAU - Gehring, Manuela
AU  - Gehring M
AD  - Department of Dermatology and Allergy, Hannover Medical School, Hannover, 
      Germany.
FAU - Patsinakidis, Nikolaos
AU  - Patsinakidis N
AD  - University Clinic of Dermatology and Allergy, University of Oldenburg, Oldenburg, 
      Germany.
FAU - Agelopoulos, Konstantin
AU  - Agelopoulos K
AD  - Department of Dermatology and Center for Chronic Pruritus, University Hospital 
      Munster, Munster, Germany.
FAU - Stander, Sonja
AU  - Stander S
AD  - Department of Dermatology and Center for Chronic Pruritus, University Hospital 
      Munster, Munster, Germany.
FAU - Homey, Bernhard
AU  - Homey B
AD  - Department of Dermatology, Heinrich-Heine-University, Dusseldorf, Germany.
FAU - Kapp, Alexander
AU  - Kapp A
AD  - Department of Dermatology and Allergy, Hannover Medical School, Hannover, 
      Germany.
FAU - Gibbs, Bernhard F
AU  - Gibbs BF
AUID- ORCID: 0000-0002-0537-228X
AD  - Division of Experimental Allergology and Immunodermatology, University of 
      Oldenburg, Oldenburg, Germany.
FAU - Ponimaskin, Evgeni
AU  - Ponimaskin E
AD  - Department of Cellular Neurophysiology, Hannover Medical School, Hannover, 
      Germany.
AD  - Institute of Cytology and Genetics, Novosibirsk, Russia.
FAU - Raap, Ulrike
AU  - Raap U
AUID- ORCID: 0000-0002-9573-4714
AD  - Division of Experimental Allergology and Immunodermatology, University of 
      Oldenburg, Oldenburg, Germany.
AD  - University Clinic of Dermatology and Allergy, University of Oldenburg, Oldenburg, 
      Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200204
PL  - England
TA  - Clin Exp Allergy
JT  - Clinical and experimental allergy : journal of the British Society for Allergy 
      and Clinical Immunology
JID - 8906443
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 7171WSG8A2 (BDNF protein, human)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Brain-Derived Neurotrophic Factor/*immunology
MH  - *Dermatitis, Atopic/immunology/pathology
MH  - *Dermis/immunology/innervation/pathology
MH  - *Eosinophils/immunology/pathology
MH  - *Epidermis/immunology/innervation/pathology
MH  - Female
MH  - Humans
MH  - Male
MH  - *Sensory Receptor Cells/immunology/pathology
OTO - NOTNLM
OT  - BDNF
OT  - DRG neurons
OT  - atopic dermatitis
OT  - eosinophils
EDAT- 2020/01/12 06:00
MHDA- 2021/08/13 06:00
CRDT- 2020/01/12 06:00
PHST- 2019/04/30 00:00 [received]
PHST- 2019/12/20 00:00 [revised]
PHST- 2020/01/03 00:00 [accepted]
PHST- 2020/01/12 06:00 [pubmed]
PHST- 2021/08/13 06:00 [medline]
PHST- 2020/01/12 06:00 [entrez]
AID - 10.1111/cea.13560 [doi]
PST - ppublish
SO  - Clin Exp Allergy. 2020 May;50(5):577-584. doi: 10.1111/cea.13560. Epub 2020 Feb 
      4.