PMID- 31927420
OWN - NLM
STAT- MEDLINE
DCOM- 20201124
LR  - 20201124
IS  - 1879-3231 (Electronic)
IS  - 0093-691X (Linking)
VI  - 144
DP  - 2020 Mar 1
TI  - Investigating the sperm parameters, oxidative stress and histopathological 
      effects of salvia miltiorrhiza hydroalcoholic extract in the prevention of 
      testicular ischemia reperfusion damage in rats.
PG  - 98-106
LID - S0093-691X(20)30002-9 [pii]
LID - 10.1016/j.theriogenology.2020.01.002 [doi]
AB  - AIMS: One of the most common urologic emergencies is spermatic cord torsion, 
      which can damage testicular tissue and reduce fertility. Salvia miltiorrhiza (SM) 
      hydroalcoholic extract possess high antioxidant properties, and its efficacy in 
      ischemia-reperfusion (I/R) injury prevention has been demonstrated in cardiac, 
      renal, and liver tissues. Therefore, the purpose of this study was to assess the 
      protective mechanism of SM extract on testicular I/R damage. MAIN METHODS: 18 
      mature male Wistar albino rats were randomly divided into 3 groups; with six rats 
      in each group: Group 1 (Sham) was sham-operated. Group 2 (T-D): torsion was 
      performed, and after 2 hours (h) detorsion was done. Group 3 (SM): 
      (200 mg kg(-1)) SM was intraperitoneally injected thirty minutes before 
      detorsion. Then testicular and epididymal weight and size alterations, sperm 
      parameters (motility, livability, concentration, and morphology), both plasma and 
      testicular tissue levels of malondialdehyde (MDA), catalase (CAT), glutathione 
      peroxidase (GPX), and total antioxidant capacity (TAC) were evaluated. Also, 
      histopathological changes included mean seminiferous tubular diameter (MSTD), 
      testicular capsule thickness (TCT), mean testicular biopsy scoring (MTBS), and 
      germinal epithelial cell thickness (GECT) were examined. RESULTS: Testicular I/R 
      significantly reduced sperm motility, viability, and normality, while SM extract 
      administration remarkably increased sperm motility, and normality (P < 0.05). 
      Induction of testicular T-D caused a significant increment in the level of MDA 
      and notable decline in the levels of GPX, CAT, and TAC both in plasma and testis 
      tissue, whereas administration of SM extract significantly decreased MDA level 
      and increased GPX, CAT, and TAC levels in plasma and testicular tissue 
      (P < 0.05). Histopathological parameters including MSTD, GECT, MTBS, and TCT were 
      significantly lower in the T-D group, while pretreatment with SM extract 
      remarkably increased MSTD, GECT, and MTBS amounts (P < 0.05). CONCLUSION: Since 
      the SM extract increased the activity of antioxidant enzymes, improved sperm 
      parameters and reduced the damage to testicular tissue, therefore, its use as a 
      potent antioxidant in reducing testicular I/R damage is suggested.
CI  - Copyright (c) 2020 Elsevier Inc. All rights reserved.
FAU - Davoodi, Farshid
AU  - Davoodi F
AD  - Department of Clinical Sciences, Faculty of Veterinary Medicine, Lorestan 
      University, Khorramabad, Iran.
FAU - Taheri, Shayan
AU  - Taheri S
AD  - Department of Clinical Sciences, Faculty of Veterinary Medicine, Lorestan 
      University, Khorramabad, Iran.
FAU - Raisi, Abbas
AU  - Raisi A
AD  - Department of Clinical Sciences, Faculty of Veterinary Medicine, Lorestan 
      University, Khorramabad, Iran. Electronic address: raisi.a@lu.ac.ir.
FAU - Rajabzadeh, Asghar
AU  - Rajabzadeh A
AD  - Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, 
      Khorramabad, Iran; Department of Anatomical Sciences, Faculty of Medicine, 
      Lorestan University of Medical Sciences, Khorramabad, Iran.
FAU - Ahmadvand, Hassan
AU  - Ahmadvand H
AD  - Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, 
      Khorramabad, Iran; Department of Biochemistry, Faculty of Medicine, Lorestan 
      University of Medical Sciences, Khorramabad, Iran.
FAU - Hablolvarid, Mohammad Hassan
AU  - Hablolvarid MH
AD  - Razi Vaccine and Serum Research Institute, Agriculture Research, Education and 
      Extension Organization (AREEO), Karaj, Iran.
FAU - Zakian, Amir
AU  - Zakian A
AD  - Department of Clinical Sciences, Faculty of Veterinary Medicine, Lorestan 
      University, Khorramabad, Iran.
LA  - eng
PT  - Journal Article
DEP - 20200103
PL  - United States
TA  - Theriogenology
JT  - Theriogenology
JID - 0421510
RN  - 0 (Biomarkers)
RN  - 0 (Plant Extracts)
SB  - IM
MH  - Animals
MH  - Biomarkers
MH  - Cell Survival
MH  - Male
MH  - Organ Size/drug effects
MH  - Oxidative Stress
MH  - Plant Extracts/chemistry/*pharmacology
MH  - Rats
MH  - *Reperfusion Injury
MH  - Salvia miltiorrhiza/*chemistry
MH  - Spermatozoa/*physiology
MH  - Testicular Diseases/etiology/*prevention & control
MH  - Testis/drug effects/*injuries/pathology
OTO - NOTNLM
OT  - Antioxidant
OT  - Ischemia reperfusion
OT  - Oxidative stress
OT  - Salvia miltiorrhiza
OT  - Sperm parameters
OT  - Testicular torsion detorsion
COIS- Declaration of competing interest Authors declare no conflict of interest.
EDAT- 2020/01/14 06:00
MHDA- 2020/11/25 06:00
CRDT- 2020/01/14 06:00
PHST- 2019/11/01 00:00 [received]
PHST- 2019/12/22 00:00 [revised]
PHST- 2020/01/01 00:00 [accepted]
PHST- 2020/01/14 06:00 [pubmed]
PHST- 2020/11/25 06:00 [medline]
PHST- 2020/01/14 06:00 [entrez]
AID - S0093-691X(20)30002-9 [pii]
AID - 10.1016/j.theriogenology.2020.01.002 [doi]
PST - ppublish
SO  - Theriogenology. 2020 Mar 1;144:98-106. doi: 10.1016/j.theriogenology.2020.01.002. 
      Epub 2020 Jan 3.