PMID- 31927658
OWN - NLM
STAT- MEDLINE
DCOM- 20210518
LR  - 20210518
IS  - 1573-6881 (Electronic)
IS  - 0145-479X (Linking)
VI  - 52
IP  - 1
DP  - 2020 Feb
TI  - The lncRNA FAL1 protects against hypoxia-reoxygenation- induced brain endothelial 
      damages through regulating PAK1.
PG  - 17-25
LID - 10.1007/s10863-019-09819-2 [doi]
AB  - Dysregulation of cerebral microvascular endothelial cells plays an important role 
      in the pathogenesis of stroke. However, the underlying mechanisms still need to 
      be elucidated. In the current study, we found that the long non-coding RNA 
      (lncRNA) FAL1 was significantly reduced in response to oxygen-glucose deprivation 
      and reoxygenation (OGD/R) stimulation in human primary brain microvascular 
      endothelial cells (HBMVECs). Interestingly, overexpression of FAL1 ameliorated 
      OGD/R-induced oxidative stress by reducing the production of reactive oxygen 
      species (ROS) and increasing the level of reduced glutathione (GSH). Also, 
      overexpression of FAL1 suppressed OGD/R-induced secretions of interleukin-6 
      (IL-6), monocyte chemotactic protein-1 (MCP-1), and high mobility group box-1 
      (HMGB-1). We then found that OGD/R-induced reduction of cell viability and 
      release of lactate dehydrogenase (LDH) were prevented by overexpression of FAL1. 
      Additionally, exposure to OGD/R significantly reduced the phosphorylated levels 
      of PAK1 and AKT as well as the total level of proliferating cell nuclear antigen 
      (PCNA), which was restored by overexpression of FAL1. Importantly, overexpression 
      of FAL1 restored OGD/R-induced reduction in the expression of endothelial nitric 
      oxide synthase (eNOS) and the subsequent release of nitric oxide (NO). Our 
      results implicate that FAL1 might be involved in the process of brain endothelial 
      cell damage.
FAU - Gao, Mingqing
AU  - Gao M
AD  - Department of Neurosurgery, The Affiliated Hospital of Wei fang Medical 
      University, No. 2428, Yuhe Road, Weifang, 261031, Shandong, China.
FAU - Fu, Jieting
AU  - Fu J
AD  - Department of Hematology, The Affiliated Hospital of Wei fang Medical University, 
      Shandong, China.
FAU - Wang, Yanqiang
AU  - Wang Y
AUID- ORCID: 0000-0002-6747-9162
AD  - Department of Neurology, The Affiliated Hospital of Wei fang Medical University, 
      Shandong, China. bingjieli025@yeah.net.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200111
PL  - United States
TA  - J Bioenerg Biomembr
JT  - Journal of bioenergetics and biomembranes
JID - 7701859
RN  - 0 (RNA, Long Noncoding)
RN  - EC 2.7.11.1 (PAK1 protein, human)
RN  - EC 2.7.11.1 (p21-Activated Kinases)
SB  - IM
MH  - Brain/*physiopathology
MH  - Cell Hypoxia
MH  - Endothelial Cells/*metabolism
MH  - Humans
MH  - RNA, Long Noncoding/*metabolism
MH  - Signal Transduction
MH  - p21-Activated Kinases/*metabolism
OTO - NOTNLM
OT  - Cerebral microvascular endothelial cells
OT  - FAL1
OT  - Oxygen-glucose deprivation and reoxygenation
OT  - Stroke
OT  - lncRNA
EDAT- 2020/01/14 06:00
MHDA- 2021/05/19 06:00
CRDT- 2020/01/14 06:00
PHST- 2019/05/28 00:00 [received]
PHST- 2019/12/12 00:00 [accepted]
PHST- 2020/01/14 06:00 [pubmed]
PHST- 2021/05/19 06:00 [medline]
PHST- 2020/01/14 06:00 [entrez]
AID - 10.1007/s10863-019-09819-2 [pii]
AID - 10.1007/s10863-019-09819-2 [doi]
PST - ppublish
SO  - J Bioenerg Biomembr. 2020 Feb;52(1):17-25. doi: 10.1007/s10863-019-09819-2. Epub 
      2020 Jan 11.