PMID- 31928703
OWN - NLM
STAT- MEDLINE
DCOM- 20201021
LR  - 20201021
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 241
DP  - 2020 Feb
TI  - Porcine reproductive and respiratory syndrome virus infection promotes C1QBP 
      secretion to enhance inflammatory responses.
PG  - 108563
LID - S0378-1135(19)31251-9 [pii]
LID - 10.1016/j.vetmic.2019.108563 [doi]
AB  - Complement component 1, q subcomponent binding protein (C1QBP) is a receptor for 
      the globular heads of C1q and modulates various biological processes including 
      infection, inflammation, autoimmunity, and cancer. In our previous study to 
      identify differentially expressed secretory proteins in Marc-145 cells infected 
      with porcine reproductive and respiratory syndrome virus (PRRSV), mass spectrum 
      data showed that C1QBP was secreted after PRRSV infection. However, the 
      biological significance of secreted C1QBP remains unclear. In this study, we 
      confirmed that PRRSV infection promoted C1QBP secretion in Marc-145 cells and 
      porcine alveolar macrophages (PAMs), the target cells of PRRSV in vivo. Knockdown 
      of endogenous C1QBP decreased PRRSV-induced inflammatory responses. The purified 
      recombinant porcine C1QBP (poC1QBP) had proinflammatory effects. The exogenous 
      addition of poC1QBP significantly enhanced PRRSV-induced inflammatory responses 
      and abolished the inhibitory effects mediated by poC1QBP-knockdown. Taken 
      together, these results demonstrate that PRRSV infection promotes poC1QBP 
      secretion that enhances inflammatory responses.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Li, Yang
AU  - Li Y
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan 430070, China; The Key 
      Laboratory of Preventive Veterinary Medicine in Hubei Province, Cooperative 
      Innovation Center for Sustainable Pig Production, Wuhan 430070, China.
FAU - Wei, Ying
AU  - Wei Y
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan 430070, China; The Key 
      Laboratory of Preventive Veterinary Medicine in Hubei Province, Cooperative 
      Innovation Center for Sustainable Pig Production, Wuhan 430070, China.
FAU - Hao, Wanjun
AU  - Hao W
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan 430070, China; The Key 
      Laboratory of Preventive Veterinary Medicine in Hubei Province, Cooperative 
      Innovation Center for Sustainable Pig Production, Wuhan 430070, China.
FAU - Zhao, Wenkai
AU  - Zhao W
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan 430070, China; The Key 
      Laboratory of Preventive Veterinary Medicine in Hubei Province, Cooperative 
      Innovation Center for Sustainable Pig Production, Wuhan 430070, China.
FAU - Zhou, Yanrong
AU  - Zhou Y
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan 430070, China; The Key 
      Laboratory of Preventive Veterinary Medicine in Hubei Province, Cooperative 
      Innovation Center for Sustainable Pig Production, Wuhan 430070, China.
FAU - Wang, Dang
AU  - Wang D
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan 430070, China; The Key 
      Laboratory of Preventive Veterinary Medicine in Hubei Province, Cooperative 
      Innovation Center for Sustainable Pig Production, Wuhan 430070, China.
FAU - Xiao, Shaobo
AU  - Xiao S
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan 430070, China; The Key 
      Laboratory of Preventive Veterinary Medicine in Hubei Province, Cooperative 
      Innovation Center for Sustainable Pig Production, Wuhan 430070, China.
FAU - Fang, Liurong
AU  - Fang L
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan 430070, China; The Key 
      Laboratory of Preventive Veterinary Medicine in Hubei Province, Cooperative 
      Innovation Center for Sustainable Pig Production, Wuhan 430070, China. Electronic 
      address: fanglr@mail.hzau.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20191223
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Cytokines)
RN  - 0 (DNA, Complementary)
RN  - 0 (Mitochondrial Proteins)
RN  - 0 (RNA, Messenger)
RN  - 80295-33-6 (Complement C1q)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Blotting, Western/veterinary
MH  - Cell Line
MH  - Chlorocebus aethiops
MH  - Cloning, Molecular
MH  - Complement C1q/genetics/*metabolism
MH  - Cytokines/metabolism
MH  - DNA, Complementary/genetics
MH  - Fluorescent Antibody Technique, Indirect/veterinary
MH  - Gene Expression Regulation, Viral
MH  - Gene Knockdown Techniques
MH  - Inflammation/metabolism
MH  - Kidney/cytology/virology
MH  - Macrophages, Alveolar/virology
MH  - Mitochondrial Proteins/*metabolism
MH  - Porcine Reproductive and Respiratory Syndrome/*metabolism/pathology
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - RNA, Messenger/metabolism
MH  - Real-Time Polymerase Chain Reaction/veterinary
MH  - Sequence Alignment/veterinary
MH  - Swine
OTO - NOTNLM
OT  - C1QBP
OT  - Inflammatory r
OT  - Porcine reproductive and respiratory syndrome virus
OT  - Secretion
OT  - esponses
EDAT- 2020/01/14 06:00
MHDA- 2020/10/22 06:00
CRDT- 2020/01/14 06:00
PHST- 2019/10/22 00:00 [received]
PHST- 2019/12/18 00:00 [revised]
PHST- 2019/12/19 00:00 [accepted]
PHST- 2020/01/14 06:00 [entrez]
PHST- 2020/01/14 06:00 [pubmed]
PHST- 2020/10/22 06:00 [medline]
AID - S0378-1135(19)31251-9 [pii]
AID - 10.1016/j.vetmic.2019.108563 [doi]
PST - ppublish
SO  - Vet Microbiol. 2020 Feb;241:108563. doi: 10.1016/j.vetmic.2019.108563. Epub 2019 
      Dec 23.