PMID- 31931595
OWN - NLM
STAT- MEDLINE
DCOM- 20200904
LR  - 20200904
IS  - 1793-6853 (Electronic)
IS  - 0192-415X (Linking)
VI  - 48
IP  - 1
DP  - 2020
TI  - Rhodiola crenulata Suppresses High Glucose-Induced Matrix Metalloproteinase 
      Expression and Inflammatory Responses by Inhibiting ROS-Related HMGB1-TLR4 
      Signaling in Endothelial Cells.
PG  - 91-105
LID - 10.1142/S0192415X20500056 [doi]
AB  - Rhodiola crenulata, a popular folk medicine for anti-altitude sickness in Tibet, 
      has been shown to have protective effects against high glucose (HG)-induced 
      endothelial cell dysfunction in human umbilical vein endothelial cells (HUVECs). 
      However, its mechanisms of action are unclear. Here, we aimed to examine the 
      effects and the mechanisms of action of Rhodiola crenulata extract (RCE) on 
      matrix metalloproteinases (MMPs) and inflammatory responses under HG conditions. 
      HUVECs were pretreated with RCE or untreated and then exposed to 33 mM glucose 
      medium for 24 h. The levels of oxidative stress markers, MMPs, endogenous tissue 
      inhibitors of MMPs (TIMPs), and adhesion molecules were determined. Zymography 
      assays were also carried out. We found that RCE significantly decreased 
      HG-induced increases in reactive oxygen species (ROS) and activation of MAPK and 
      NF-kappaB pathways. In addition, RCE not only significantly reduced the expression 
      and activities of MMPs but also upregulated TIMP protein levels. Consistently, 
      HG-induced activation of the toll-like receptor 4 (TLR4)/myeloid differentiation 
      primary response protein (MyD88) signaling pathway, intracellular adhesion 
      molecule-1 (ICAM-1), vascular adhesion molecule-1 (VCAM-1), and high mobility 
      group box 1 (HMGB1) as well as endothelial cell apoptosis was inhibited by RCE 
      treatment. RCE exerts protective effects on endothelial cells against HG insult, 
      partially by suppressing the HMGB1/TLR4 axis. These findings indicate that 
      Rhodiola crenulata may be a potential therapeutic agent for diabetes-associated 
      vascular diseases.
FAU - Huang, Li-Yen
AU  - Huang LY
AD  - Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, 
      Taiwan.
AD  - Division of Cardiology, Department of Internal Medicine, Taoyuan Armed Forces 
      General Hospital, Taoyuan, Taiwan.
FAU - Yen, I-Chuan
AU  - Yen IC
AD  - School of Pharmacy, National Defense Medical Center, Taipei, Taiwan.
FAU - Tsai, Wei-Cheng
AU  - Tsai WC
AD  - Graduate Institute of Aerospace and Undersea Medicine, National Defense Medical 
      Center, Taipei, Taiwan.
FAU - Lee, Shih-Yu
AU  - Lee SY
AD  - Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, 
      Taiwan.
AD  - Graduate Institute of Aerospace and Undersea Medicine, National Defense Medical 
      Center, Taipei, Taiwan.
LA  - eng
PT  - Journal Article
DEP - 20200114
PL  - Singapore
TA  - Am J Chin Med
JT  - The American journal of Chinese medicine
JID - 7901431
RN  - 0 (HMGB1 Protein)
RN  - 0 (HMGB1 protein, human)
RN  - 0 (Plant Extracts)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (TLR4 protein, human)
RN  - 0 (Toll-Like Receptor 4)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Cells, Cultured
MH  - Glucose/metabolism
MH  - HMGB1 Protein/*metabolism
MH  - Human Umbilical Vein Endothelial Cells/drug effects
MH  - Humans
MH  - Matrix Metalloproteinases/*metabolism
MH  - Oxidative Stress/*drug effects
MH  - Plant Extracts/*pharmacology
MH  - Reactive Oxygen Species/metabolism
MH  - Rhodiola/*chemistry
MH  - Toll-Like Receptor 4/*metabolism
OTO - NOTNLM
OT  - Endothelial Cells
OT  - High Glucose
OT  - High Mobility Group Box 1
OT  - Matrix Metalloproteinases
OT  - Rhodiola crenulata
OT  - Toll-Like Receptor 4
EDAT- 2020/01/15 06:00
MHDA- 2020/09/05 06:00
CRDT- 2020/01/15 06:00
PHST- 2020/01/15 06:00 [pubmed]
PHST- 2020/09/05 06:00 [medline]
PHST- 2020/01/15 06:00 [entrez]
AID - 10.1142/S0192415X20500056 [doi]
PST - ppublish
SO  - Am J Chin Med. 2020;48(1):91-105. doi: 10.1142/S0192415X20500056. Epub 2020 Jan 
      14.