PMID- 31932027
OWN - NLM
STAT- MEDLINE
DCOM- 20201029
LR  - 20201029
IS  - 1532-818X (Electronic)
IS  - 0196-0709 (Linking)
VI  - 41
IP  - 3
DP  - 2020 May-Jun
TI  - Identification of MMP1 and MMP2 by RNA-seq analysis in laryngeal squamous cell 
      carcinoma.
PG  - 102391
LID - S0196-0709(19)30811-7 [pii]
LID - 10.1016/j.amjoto.2020.102391 [doi]
AB  - BACKGROUND: Laryngeal squamous cell carcinoma (LSCC) is the most common 
      histologic subtype of laryngeal cancer characterized by a poor prognosis. 
      Determining gene expression changes in LSCC should improve our understanding of 
      putative risk factors and provide potential targets for therapy. OBJECTIVES: To 
      assess differential gene expression between LSCC tissue and paired normal 
      laryngeal tissue, and to provide gene targets for future studies of this type of 
      laryngeal cancer. MATERIALS AND METHODS: Three paired-sample groups (tumor and 
      normal tissue) from patients with laryngeal squamous cell carcinoma were analyzed 
      by RNA sequencing (RNA-seq). RESULTS: The six cDNA libraries generated raw reads 
      ranging from 15,195,586 to 21,443,488 counts. Changes in gene expression levels 
      were determined in 40,205 of these counts, with 18,466 deferentially expressed 
      genes in all three groups. Compared to normal tissue, the expression levels of 
      MMP1 and MMP2 increased significantly in tumor tissue of patients with laryngeal 
      squamous cell carcinoma. CONCLUSIONS: Whole transcriptome sequencing revealed 
      that MMP1 and MMP2 are highly expressed in LSCC. These genes may be useful both 
      as biomarkers for LSCC diagnosis and as targets for therapy, as well as for 
      increasing our understanding of LSCC tumorigenesis.
CI  - Copyright (c) 2020 Elsevier Inc. All rights reserved.
FAU - Fang, Weijun
AU  - Fang W
AD  - Emergency Center, Zhongnan Hospital of Wuhan University, Wuhan 430071, China. 
      Electronic address: fwj20140906@163.com.
FAU - Shen, Jun
AU  - Shen J
AD  - Emergency Center, Zhongnan Hospital of Wuhan University, Wuhan 430071, China.
LA  - eng
PT  - Journal Article
DEP - 20200107
PL  - United States
TA  - Am J Otolaryngol
JT  - American journal of otolaryngology
JID - 8000029
RN  - 0 (Biomarkers, Tumor)
RN  - EC 3.4.24.24 (MMP2 protein, human)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.7 (MMP1 protein, human)
RN  - EC 3.4.24.7 (Matrix Metalloproteinase 1)
SB  - IM
MH  - Aged
MH  - Biomarkers, Tumor/genetics/metabolism
MH  - Carcinogenesis/*genetics
MH  - Carcinoma, Squamous Cell/diagnosis/*genetics/metabolism/pathology
MH  - *Gene Expression
MH  - Gene Expression Regulation, Neoplastic/*genetics
MH  - Humans
MH  - Laryngeal Neoplasms/diagnosis/*genetics/metabolism/pathology
MH  - Male
MH  - Matrix Metalloproteinase 1/*genetics/metabolism
MH  - Matrix Metalloproteinase 2/*genetics/metabolism
MH  - Middle Aged
MH  - Molecular Targeted Therapy
MH  - Prognosis
MH  - Sequence Analysis, RNA
OTO - NOTNLM
OT  - LSCC
OT  - MMP1
OT  - MMP2
OT  - RNA-seq
COIS- Declaration of competing interest There are no conflicts of interest to declare.
EDAT- 2020/01/15 06:00
MHDA- 2020/10/30 06:00
CRDT- 2020/01/15 06:00
PHST- 2019/09/20 00:00 [received]
PHST- 2019/12/19 00:00 [revised]
PHST- 2020/01/03 00:00 [accepted]
PHST- 2020/01/15 06:00 [pubmed]
PHST- 2020/10/30 06:00 [medline]
PHST- 2020/01/15 06:00 [entrez]
AID - S0196-0709(19)30811-7 [pii]
AID - 10.1016/j.amjoto.2020.102391 [doi]
PST - ppublish
SO  - Am J Otolaryngol. 2020 May-Jun;41(3):102391. doi: 10.1016/j.amjoto.2020.102391. 
      Epub 2020 Jan 7.