PMID- 31933724
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200117
IS  - 1936-2625 (Electronic)
IS  - 1936-2625 (Linking)
VI  - 12
IP  - 1
DP  - 2019
TI  - NF-kappaB/Twist axis is involved in chysin inhibition of ovarian cancer stem cell 
      features induced by co-treatment of TNF-alpha and TGF-beta.
PG  - 101-112
AB  - Chrysin (ChR) inhibits various cancer cells and possesses anti-inflammatory 
      activities. NF-kB has been shown to regulate the expression of genes involved in 
      epithelial-mesenchymal transformation (EMT) by upregulation of TWIST1. This study 
      aimed to assess whether ChR can inhibit EMT phenotype and cancer stem-like cell 
      (CSLC) features in ovarian cancer cells co-treated with TNF-alpha and TGF-beta. Here, 
      OVCAR-3 cells were co-treated with TNF-alpha and TGF-beta in the presence or absence of 
      ChR. Then, the expression levels of E-cadherin, N-cadherin, CD133, CD44, 
      NF-kappaBp65, and TWIST1 were analyzed by western blotting. Wound healing and tumor 
      sphere formation assays were performed to assess the migration and sphere-forming 
      capabilities of cells, respectively. Overexpression and/or knockdown of NF-kappaBp65 
      and/or TWIST1 were used to explore the molecular mechanisms. We showed that ChR 
      inhibited EMT and CSLC properties in ovarian cancer cells administered TNF-alpha 
      after prolonged TGF-beta treatment, in a dose-dependent manner. Also, knockdown of 
      NF-kappaBp65 and ChR cooperatively enhanced the inhibition of NF-kappaBp65 and TWIST1 
      expression, EMT phenotype, and CSLC properties. Conversely, overexpression of 
      NF-kappaBp65 antagonized the above-mentioned activities of ChR. Furthermore, TWIST1 
      silencing or overexpression did not affect the ChR treatment effect on NF-kappaBp65 
      levels, but it reduced or enhanced EMT and CSLC properties. In conclusion, ChR 
      can inhibit a proinflammatory cytokine to induce EMT and CSLC characteristics in 
      OVCAR-3 cells, which may be involved in blocking the NF-kappaB/Twist axis.
CI  - IJCEP Copyright (c) 2019.
FAU - Li, Huazhen
AU  - Li H
AD  - Department of Gynecology and Obstetrics, The Second Affiliated Hospital of 
      Guangzhou Medical University 511447, Guangdong, China.
FAU - Chen, A
AU  - Chen A
AD  - Department of Pharmaceutical Science, Medical College, Hunan Normal University, 
      Key Laboratory of Study and Discovery of Small Targeted Molecules of Hunan 
      Province Changsha 410013, China.
FAU - Yuan, Qiaoqi
AU  - Yuan Q
AD  - Department of Gynecology and Obstetrics, The Second Affiliated Hospital of 
      Guangzhou Medical University 511447, Guangdong, China.
FAU - Chen, Weifeng
AU  - Chen W
AD  - Department of Gynecology and Obstetrics, The Second Affiliated Hospital of 
      Guangzhou Medical University 511447, Guangdong, China.
FAU - Zhong, Huimin
AU  - Zhong H
AD  - Department of Gynecology and Obstetrics, The Second Affiliated Hospital of 
      Guangzhou Medical University 511447, Guangdong, China.
FAU - Teng, Mo
AU  - Teng M
AD  - Department of Gynecology and Obstetrics, The Second Affiliated Hospital of 
      Guangzhou Medical University 511447, Guangdong, China.
FAU - Xu, Chang
AU  - Xu C
AD  - Department of Pharmaceutical Science, Medical College, Hunan Normal University, 
      Key Laboratory of Study and Discovery of Small Targeted Molecules of Hunan 
      Province Changsha 410013, China.
FAU - Qiu, Yebei
AU  - Qiu Y
AD  - Department of Pharmaceutical Science, Medical College, Hunan Normal University, 
      Key Laboratory of Study and Discovery of Small Targeted Molecules of Hunan 
      Province Changsha 410013, China.
FAU - Cao, Jianguo
AU  - Cao J
AD  - Department of Pharmaceutical Science, Medical College, Hunan Normal University, 
      Key Laboratory of Study and Discovery of Small Targeted Molecules of Hunan 
      Province Changsha 410013, China.
LA  - eng
PT  - Journal Article
DEP - 20190101
PL  - United States
TA  - Int J Clin Exp Pathol
JT  - International journal of clinical and experimental pathology
JID - 101480565
PMC - PMC6943996
OTO - NOTNLM
OT  - NF-kappaB
OT  - Ovarian cancer
OT  - TWIST1
OT  - cancer stem cells
OT  - chrysin
OT  - epithelial-mesenchymal transition
COIS- None.
EDAT- 2020/01/15 06:00
MHDA- 2020/01/15 06:01
PMCR- 2019/01/01
CRDT- 2020/01/15 06:00
PHST- 2018/09/08 00:00 [received]
PHST- 2018/11/06 00:00 [accepted]
PHST- 2020/01/15 06:00 [entrez]
PHST- 2020/01/15 06:00 [pubmed]
PHST- 2020/01/15 06:01 [medline]
PHST- 2019/01/01 00:00 [pmc-release]
PST - epublish
SO  - Int J Clin Exp Pathol. 2019 Jan 1;12(1):101-112. eCollection 2019.