PMID- 31935500
OWN - NLM
STAT- MEDLINE
DCOM- 20200227
LR  - 20201019
IS  - 1879-0038 (Electronic)
IS  - 0378-1119 (Linking)
VI  - 731
DP  - 2020 Mar 20
TI  - Elevated expression of IL-18 but not IL-1beta gene is associated with NALP3 and AIM2 
      inflammasome in Polycystic Ovary Syndrome.
PG  - 144352
LID - S0378-1119(20)30021-4 [pii]
LID - 10.1016/j.gene.2020.144352 [doi]
AB  - Inflammasome complex mediated interleukin 1beta (IL-1beta) and interleukin 18 (IL-18) 
      production may be involved in immunopathogenesis of polycystic ovary syndrome 
      (PCOS). Therefore, this study was conducted to investigate involved inflammasome 
      pathways in PCOS. Therefore, inflammasome genes expression and serum level of 
      IL-1beta were evaluated in 30 patients with confirmed PCOS and 30 women without 
      PCOS. A remarkable increase in expression of the nucleotide binding and 
      oligomerization domain (NOD)-like receptor (NLR) family pyrin domain-containing 3 
      (NALP3), absent in melanoma 2 (AIM2), IL-18 and associated speck-like protein 
      containing a caspase recruitment domain (CARD); (ASC) genes in PCOS were observed 
      (p < 0.05). In contrast, expression level of NALP1, NALP12, NLR family apoptosis 
      inhibitory proteins (NAIP), NLR family caspase recruitment domain (CARD) domain 
      containing 4 (NLRC4) and IL-1beta genes was not significant. Although the IL-1beta 
      protein level in serum of COS patients with BMI >/= 25 was significantly higher 
      than PCOS patient with BMI < 25, but there was no significant difference in 
      non-PCOS individuals with BMI < 25 or >/=25. Furthermore, significant correlation 
      between expression of AIM2 (r = 0.83, p = 0.032) and NALP3 (r = 0.59, p = 0.0001) 
      was observed with IL-18, while a positive correlation (r = 0.84, p = 0.0001) was 
      revealed between NAIP and IL-1beta. Based on the obtained results on inflammasome 
      components along with increased expression of IL-1beta especially in overweight 
      patients, it can be concluded that IL-18 expression as well as IL-1beta is probably 
      due to activation of AIM2, NALP3 or NAIP inflammasome, which may play a critical 
      role in immunopathology of PCOS.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Rostamtabar, Maryam
AU  - Rostamtabar M
AD  - Student Research Committee, Babol University of Medical Sciences, Babol, Iran.
FAU - Esmaeilzadeh, Sedighe
AU  - Esmaeilzadeh S
AD  - Infertility and Reproductive Health Research Center, Health Research Institute, 
      Babol University of Medical Science, Babol, Iran.
FAU - Karkhah, Ahmad
AU  - Karkhah A
AD  - Student Research Committee, Babol University of Medical Sciences, Babol, Iran; 
      Cellular and Molecular Biology Research Center, Health Research Institute, Babol 
      University of Medical Sciences, Babol, Iran.
FAU - Amiri, Mania
AU  - Amiri M
AD  - Infertility and Reproductive Health Research Center, Health Research Institute, 
      Babol University of Medical Science, Babol, Iran.
FAU - Rahmani, Abolfazl
AU  - Rahmani A
AD  - Student Research Committee, Babol University of Medical Sciences, Babol, Iran.
FAU - Bakouei, Fatemeh
AU  - Bakouei F
AD  - Infertility and Reproductive Health Research Center, Health Research Institute, 
      Babol University of Medical Science, Babol, Iran.
FAU - Nouri, Hamid Reza
AU  - Nouri HR
AD  - Infertility and Reproductive Health Research Center, Health Research Institute, 
      Babol University of Medical Science, Babol, Iran; Cellular and Molecular Biology 
      Research Center, Health Research Institute, Babol University of Medical Sciences, 
      Babol, Iran; Immunoregulation Research Center, Health Research Institute, Babol 
      University of Medical Sciences, Babol, Iran. Electronic address: 
      h.noori@mubabol.ac.ir.
LA  - eng
PT  - Journal Article
DEP - 20200111
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (AIM2 protein, human)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (IL18 protein, human)
RN  - 0 (IL1B protein, human)
RN  - 0 (Inflammasomes)
RN  - 0 (Interleukin-18)
RN  - 0 (Interleukin-1beta)
RN  - 0 (NLR Family, Pyrin Domain-Containing 3 Protein)
RN  - 0 (NLRP3 protein, human)
SB  - IM
MH  - Adult
MH  - Case-Control Studies
MH  - DNA-Binding Proteins/metabolism
MH  - Female
MH  - Humans
MH  - Inflammasomes/*metabolism
MH  - Interleukin-18/blood/*genetics/metabolism
MH  - Interleukin-1beta/blood/*genetics/metabolism
MH  - NLR Family, Pyrin Domain-Containing 3 Protein/metabolism
MH  - Polycystic Ovary Syndrome/blood/*genetics/immunology/*metabolism
MH  - Young Adult
OTO - NOTNLM
OT  - IL-18
OT  - IL-1beta
OT  - Inflammasome
OT  - Polycystic Ovary Syndrome
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2020/01/15 06:00
MHDA- 2020/02/28 06:00
CRDT- 2020/01/15 06:00
PHST- 2019/08/24 00:00 [received]
PHST- 2020/01/07 00:00 [revised]
PHST- 2020/01/08 00:00 [accepted]
PHST- 2020/01/15 06:00 [pubmed]
PHST- 2020/02/28 06:00 [medline]
PHST- 2020/01/15 06:00 [entrez]
AID - S0378-1119(20)30021-4 [pii]
AID - 10.1016/j.gene.2020.144352 [doi]
PST - ppublish
SO  - Gene. 2020 Mar 20;731:144352. doi: 10.1016/j.gene.2020.144352. Epub 2020 Jan 11.