PMID- 31935958
OWN - NLM
STAT- MEDLINE
DCOM- 20200701
LR  - 20221207
IS  - 1660-4601 (Electronic)
IS  - 1661-7827 (Print)
IS  - 1660-4601 (Linking)
VI  - 17
IP  - 1
DP  - 2020 Jan 6
TI  - Density-Based Separation of Microbial Functional Groups in Activated Sludge.
LID - 10.3390/ijerph17010376 [doi]
LID - 376
AB  - Mechanistic understanding of how activated sludge (AS) solids density influences 
      wastewater treatment processing is limited. Because microbial groups often 
      generate and store intracellular inclusions during certain metabolic processes, 
      it is hypothesized that some microorganisms, like polyphosphate-accumulating 
      organisms (PAOs), would have higher biomass densities. The present study 
      developed a density-based separation approach and applied it to suspended growth 
      AS in two full-scale domestic water resource recovery facilities (WRRFs). 
      Incorporating quantitative real-time PCR (qPCR) and fluorescence in situ 
      hybridization (FISH) analyses, the research demonstrated the effectiveness of 
      density-based separation in enriching key microbial functional groups, including 
      ammonia-oxidizing bacteria (AOB), nitrite-oxidizing bacteria (NOB) and PAOs, by 
      up to 90-fold in target biomass fractions. It was observed that WRRF process 
      functionalities have significant influence on density-based enrichment, such that 
      maximum enrichments were achieved in the sludge fraction denser than 1.036 
      g/cm(3) for the enhanced biological phosphorus removal (EBPR) facility and in the 
      sludge fraction lighter than 1.030 g/cm(3) for the non-EBPR facility. Our results 
      provide important information on the relationship between biomass density and 
      enrichment of microbial functional groups in AS, contributing to future designs 
      of enhanced biological treatment processes for improved AS settleability and 
      performance.
FAU - Li, Lin
AU  - Li L
AD  - Department of Civil and Environmental Engineering, University of Nevada, Reno, NV 
      89557, USA.
FAU - You, Yaqi
AU  - You Y
AD  - Department of Civil and Environmental Engineering, University of Nevada, Reno, NV 
      89557, USA.
FAU - Pagilla, Krishna
AU  - Pagilla K
AD  - Department of Civil and Environmental Engineering, University of Nevada, Reno, NV 
      89557, USA.
LA  - eng
PT  - Journal Article
DEP - 20200106
PL  - Switzerland
TA  - Int J Environ Res Public Health
JT  - International journal of environmental research and public health
JID - 101238455
RN  - 0 (Polyphosphates)
RN  - 0 (Sewage)
RN  - 0 (Waste Water)
RN  - 27YLU75U4W (Phosphorus)
SB  - IM
MH  - Bacteria/*classification/*metabolism
MH  - Biomass
MH  - Bioreactors/*microbiology
MH  - In Situ Hybridization, Fluorescence
MH  - Phosphorus/metabolism
MH  - Polyphosphates/metabolism
MH  - Sewage/*microbiology
MH  - Waste Disposal, Fluid
MH  - Wastewater/microbiology
PMC - PMC6981482
OTO - NOTNLM
OT  - FISH
OT  - activated sludge
OT  - ammonia-oxidizing bacteria (AOB)
OT  - density-based separation
OT  - enrichment of microbial functional groups
OT  - nitrite-oxidizing bacterial (NOB)
OT  - phosphate accumulating organisms (PAOs)
OT  - qPCR
COIS- The authors declare no conflict of interest.
EDAT- 2020/01/16 06:00
MHDA- 2020/07/02 06:00
PMCR- 2020/01/01
CRDT- 2020/01/16 06:00
PHST- 2019/11/15 00:00 [received]
PHST- 2019/12/20 00:00 [revised]
PHST- 2019/12/27 00:00 [accepted]
PHST- 2020/01/16 06:00 [entrez]
PHST- 2020/01/16 06:00 [pubmed]
PHST- 2020/07/02 06:00 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - ijerph17010376 [pii]
AID - ijerph-17-00376 [pii]
AID - 10.3390/ijerph17010376 [doi]
PST - epublish
SO  - Int J Environ Res Public Health. 2020 Jan 6;17(1):376. doi: 
      10.3390/ijerph17010376.