PMID- 31938018
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220412
IS  - 1475-2867 (Print)
IS  - 1475-2867 (Electronic)
IS  - 1475-2867 (Linking)
VI  - 20
DP  - 2020
TI  - LINC00908 negatively regulates microRNA-483-5p to increase TSPYL5 expression and 
      inhibit the development of prostate cancer.
PG  - 10
LID - 10.1186/s12935-019-1073-x [doi]
LID - 10
AB  - BACKGROUND: Accumulating evidence has associated aberrant long non-coding RNAs 
      (lncRNAs) with various human cancers. This study aimed to explore the role of 
      LINC00908 in prostate cancer (PCa) and its possible underlying mechanisms. 
      METHODS: Microarray data associated with PCa were obtained from the Gene 
      Expression Omnibus (GEO) to screen the differentially expressed genes or lncRNAs. 
      Then, the expression of LINC00908 in PCa tissues and cell lines was detected by 
      reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The 
      localization of LINC00908 in PCa cells was examined by fluorescence in situ 
      hybridization (FISH). The relationship among LINC00908, microRNA (miR)-483-5p, 
      and TSPYL5 was detected by bioinformatics analysis, dual-luciferase reporter 
      assay, RNA pull-down, RNA binding protein immunoprecipitation (RIP), and FISH 
      assays. Cell biological behaviors were assessed after the expression of 
      LINC00908, miR-483-5p, and TSPYL5 was altered in PCa cells. Lastly, tumor growth 
      in nude mice was evaluated. RESULTS: Poorly expressed LINC00908 was witnessed in 
      PCa tissues and cells. LINC00908 competitively bound to miR-483-5p to up-regulate 
      the TSPYL5 expression. Overexpression of LINC00908 resulted in reduced PCa cell 
      proliferation, migration and invasion, and promoted apoptosis. Additionally, the 
      suppression on PCa cell proliferation, migration and invasion was induced by 
      up-regulation of TSPYL5 or inhibition of miR-483-5p. In addition, in vivo 
      experiments showed that overexpression of LINC00908 inhibited tumor growth of 
      PCa. CONCLUSION: Overall, LINC00908 could competitively bind to miR-483-5p to 
      increase the expression of TSPYL5, thereby inhibiting the progression of PCa. 
      Therefore, LINC00908 may serve as a novel target for the treatment of PCa.
CI  - (c) The Author(s) 2020.
FAU - Fan, Li
AU  - Fan L
AD  - Department of Urology, China-Japan Union Hospital of Jilin University, No. 126, 
      Xiantai Street, Changchun, 130033 Jilin People's Republic of China. ISNI: 0000 
      0004 1771 3349. GRID: grid.415954.8
FAU - Li, Hai
AU  - Li H
AD  - Department of Urology, China-Japan Union Hospital of Jilin University, No. 126, 
      Xiantai Street, Changchun, 130033 Jilin People's Republic of China. ISNI: 0000 
      0004 1771 3349. GRID: grid.415954.8
FAU - Zhang, Yun
AU  - Zhang Y
AD  - Department of Urology, China-Japan Union Hospital of Jilin University, No. 126, 
      Xiantai Street, Changchun, 130033 Jilin People's Republic of China. ISNI: 0000 
      0004 1771 3349. GRID: grid.415954.8
LA  - eng
PT  - Journal Article
DEP - 20200109
PL  - England
TA  - Cancer Cell Int
JT  - Cancer cell international
JID - 101139795
PMC - PMC6953146
OTO - NOTNLM
OT  - Apoptosis
OT  - Invasion
OT  - LINC00908
OT  - Migration
OT  - Proliferation
OT  - Prostate cancer
OT  - TSPYL5
OT  - microRNA-483-5p
COIS- Competing interestsThe authors declare that they have no competing interests.
EDAT- 2020/01/16 06:00
MHDA- 2020/01/16 06:01
PMCR- 2020/01/09
CRDT- 2020/01/16 06:00
PHST- 2019/08/14 00:00 [received]
PHST- 2019/12/13 00:00 [accepted]
PHST- 2020/01/16 06:00 [entrez]
PHST- 2020/01/16 06:00 [pubmed]
PHST- 2020/01/16 06:01 [medline]
PHST- 2020/01/09 00:00 [pmc-release]
AID - 1073 [pii]
AID - 10.1186/s12935-019-1073-x [doi]
PST - epublish
SO  - Cancer Cell Int. 2020 Jan 9;20:10. doi: 10.1186/s12935-019-1073-x. eCollection 
      2020.