PMID- 31938022
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220412
IS  - 1475-2867 (Print)
IS  - 1475-2867 (Electronic)
IS  - 1475-2867 (Linking)
VI  - 20
DP  - 2020
TI  - Silencing of miR-17-5p suppresses cell proliferation and promotes cell apoptosis 
      by directly targeting PIK3R1 in laryngeal squamous cell carcinoma.
PG  - 14
LID - 10.1186/s12935-020-1096-3 [doi]
LID - 14
AB  - BACKGROUND: Increasing evidence has suggested that microRNAs (miRNAs) act as key 
      post-transcriptional regulators in tumor progression. Previous studies have 
      confirmed that miR-17-5p functions as an oncogene in multiple cancers and 
      contributes to tumor progression. However, the role and biological functions of 
      miR-17-5p in the development of laryngeal squamous cell carcinoma (LSCC) still 
      remain unknown. METHODS: qRT-PCR was used to detect miRNA and mRNA expression 
      levels in LSCC tissues and cell lines. CCK-8 assay was used to measure cell 
      viability and flow cytometry was performed to evaluate cell apoptosis. Western 
      blot analysis was used to detect the protein levels of BAX, BCL-2, cleaved 
      Caspase-3, PIK3R1 and AKT. Luciferase reporter assay was used to detect the 
      effect of miR-17-5p on PIK3R1 expression. Xenograft animal model was used to test 
      the effect of miR-17-5p on LSCC cell in vivo. RESULTS: In the present study, we 
      found that miR-17-5p expression level was upregulated in LSCC tissues and cell 
      lines. Depletion of miR-17-5p in LSCC cells significantly reduced cell 
      proliferation and promoted cell apoptosis in vitro and in vivo. Mechanically, 
      knockdown of miR-17-5p in LSCC cells inhibited BCL-2 expression while enhanced 
      BAX and cleaved Caspase-3 protein expression. Moreover, depletion of miR-17-5p in 
      LSCC cells suppressed AKT phosphorylation but did not influence PTEN expression. 
      Importantly, miR-17-5p positively regulated PIK3R1 expression by directly binding 
      to its 3'-untranslated region (UTR). Additionally, PIK3R1, which expression was 
      downregulated in LSCC tissues and cell lines, was involved in LSCC cell survival 
      by modulating the activation of AKT signal pathway. Dysregulation of 
      miR-17-5p/PIK3R1 axis was participated in LSCC cell proliferation and apoptosis 
      by inhibiting the activation of the PI3K/AKT signaling pathway. CONCLUSIONS: In 
      conclusion, our study indicates that the miR-17-5p/PIK3R1 axis plays an essential 
      role in the development of LSCC and provides a potential therapeutic target for 
      LSCC treatment.
CI  - (c) The Author(s) 2020.
FAU - Wang, Jian-Xing
AU  - Wang JX
AD  - 1Department of Otolaryngology, The Second Hospital of Hebei Medical University, 
      215 Heping West Road, Shijiazhuang, 050000 People's Republic of China. ISNI: 0000 
      0004 1804 3009. GRID: grid.452702.6
FAU - Jia, Xin-Ju
AU  - Jia XJ
AD  - 2Department of Endocrinology, The First Hospital of Hebei Medical University, 89 
      Donggang Road, Shijiazhuang, 050000 People's Republic of China. GRID: 
      grid.452458.a
FAU - Liu, Yan
AU  - Liu Y
AD  - 3Department of Anesthesiology, The 4th Hospital of Hebei Medical University, 169 
      Tianshan Street, Shijiazhuang, 050000 People's Republic of China. ISNI: 0000 0004 
      1760 8442. GRID: grid.256883.2
FAU - Dong, Jin-Hui
AU  - Dong JH
AD  - 1Department of Otolaryngology, The Second Hospital of Hebei Medical University, 
      215 Heping West Road, Shijiazhuang, 050000 People's Republic of China. ISNI: 0000 
      0004 1804 3009. GRID: grid.452702.6
FAU - Ren, Xiu-Min
AU  - Ren XM
AD  - 1Department of Otolaryngology, The Second Hospital of Hebei Medical University, 
      215 Heping West Road, Shijiazhuang, 050000 People's Republic of China. ISNI: 0000 
      0004 1804 3009. GRID: grid.452702.6
FAU - Xu, Ou
AU  - Xu O
AD  - 1Department of Otolaryngology, The Second Hospital of Hebei Medical University, 
      215 Heping West Road, Shijiazhuang, 050000 People's Republic of China. ISNI: 0000 
      0004 1804 3009. GRID: grid.452702.6
FAU - Liu, Sheng-Hui
AU  - Liu SH
AD  - 4Department of Otolaryngology Head and Neck Surgery, The 4th Hospital of Hebei 
      Medical University, 169 Tianshan Street, Shijiazhuang, 050000 People's Republic 
      of China. ISNI: 0000 0004 1760 8442. GRID: grid.256883.2
FAU - Shan, Chun-Guang
AU  - Shan CG
AUID- ORCID: 0000-0002-8378-5469
AD  - 1Department of Otolaryngology, The Second Hospital of Hebei Medical University, 
      215 Heping West Road, Shijiazhuang, 050000 People's Republic of China. ISNI: 0000 
      0004 1804 3009. GRID: grid.452702.6
LA  - eng
PT  - Journal Article
DEP - 20200110
PL  - England
TA  - Cancer Cell Int
JT  - Cancer cell international
JID - 101139795
PMC - PMC6954602
OTO - NOTNLM
OT  - Apoptosis
OT  - Laryngeal squamous cell carcinoma
OT  - PIK3R1
OT  - Proliferation
OT  - miR-17-5p
COIS- Competing interestsThe authors declare that they have no competing interests.
EDAT- 2020/01/16 06:00
MHDA- 2020/01/16 06:01
PMCR- 2020/01/10
CRDT- 2020/01/16 06:00
PHST- 2019/11/01 00:00 [received]
PHST- 2020/01/02 00:00 [accepted]
PHST- 2020/01/16 06:00 [entrez]
PHST- 2020/01/16 06:00 [pubmed]
PHST- 2020/01/16 06:01 [medline]
PHST- 2020/01/10 00:00 [pmc-release]
AID - 1096 [pii]
AID - 10.1186/s12935-020-1096-3 [doi]
PST - epublish
SO  - Cancer Cell Int. 2020 Jan 10;20:14. doi: 10.1186/s12935-020-1096-3. eCollection 
      2020.