PMID- 31938242
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200325
IS  - 1936-2625 (Electronic)
IS  - 1936-2625 (Linking)
VI  - 11
IP  - 3
DP  - 2018
TI  - Human umbilical cord mesenchymal stem cells polarize RAW264.7 macrophages to an 
      anti-inflammatory subpopulation.
PG  - 1446-1452
AB  - OBJECTIVE: To investigate the effects of human umbilical cord mesenchymal stem 
      cells (hUCMSCs) on the polarization of lipopolysaccharide-stimulated RAW264.7 
      macrophages. METHODS: Lipopolysaccharide-stimulated RAW264.7 macrophages were 
      co-cultured with hUCMSCs in a Transwell system for 4 d, and then labelled with 
      anti-F4/80, anti-CD86, and anti-CD206 antibodies for flow cytometry. The 
      co-cultured supernatants were detected by enzyme-linked immunosorbent assay for 
      prostaglandin E2. The co-cultured RAW264.7 macrophages were also lysed to measure 
      the intracellular level of inducible nitric oxide synthase. RESULTS: There were 
      significantly more F4/80+CD86+CD206+ RAW264.7 macrophages in the hUCMSCs-treated 
      groups than the control group (P<0.001). The secretion of prostaglandin E2 by 
      lipopolysaccharide-stimulated RAW264.7 macrophages was significantly inhibited in 
      a dose-dependent manner with the addition of hUCMSCs (P<0.001). The expression of 
      iNOS, the intracellular marker of M1 cells, was also significantly inhibited by 
      hUCMSCs (P<0.05). CONCLUSION: hUCMSCs significantly polarize the 
      lipopolysaccharide-stimulated RAW264.7 macrophages from a pro-inflammatory M1 
      subpopulation to an intermediate subpopulation of anti-inflammatory M2 
      macrophages, which are associated with a gradual decrease of iNOS and PGE(2) 
      levels.
CI  - IJCEP Copyright (c) 2018.
FAU - Xu, Yu
AU  - Xu Y
AD  - Department of Cardiology, Zhengzhou University People's Hospital Zhengzhou, 
      Henan, China.
FAU - Luo, Hongxing
AU  - Luo H
AD  - Department of Cardiology, Zhengzhou University People's Hospital Zhengzhou, 
      Henan, China.
FAU - Chen, Fang
AU  - Chen F
AD  - Department of Cardiology, Zhengzhou University People's Hospital Zhengzhou, 
      Henan, China.
FAU - Shi, Ya'nan
AU  - Shi Y
AD  - Department of Cardiology, Zhengzhou University People's Hospital Zhengzhou, 
      Henan, China.
FAU - Sun, Mengna
AU  - Sun M
AD  - Department of Cardiology, Zhengzhou University People's Hospital Zhengzhou, 
      Henan, China.
LA  - eng
PT  - Journal Article
DEP - 20180301
PL  - United States
TA  - Int J Clin Exp Pathol
JT  - International journal of clinical and experimental pathology
JID - 101480565
PMC - PMC6958167
OTO - NOTNLM
OT  - Human umbilical cord mesenchymal stem cell
OT  - macrophage polarization
OT  - prostaglandin E2
COIS- None.
EDAT- 2018/03/01 00:00
MHDA- 2018/03/01 00:01
PMCR- 2018/03/01
CRDT- 2020/01/16 06:00
PHST- 2017/11/23 00:00 [received]
PHST- 2017/12/17 00:00 [accepted]
PHST- 2020/01/16 06:00 [entrez]
PHST- 2018/03/01 00:00 [pubmed]
PHST- 2018/03/01 00:01 [medline]
PHST- 2018/03/01 00:00 [pmc-release]
PST - epublish
SO  - Int J Clin Exp Pathol. 2018 Mar 1;11(3):1446-1452. eCollection 2018.