PMID- 31938307
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200119
IS  - 1936-2625 (Electronic)
IS  - 1936-2625 (Linking)
VI  - 11
IP  - 4
DP  - 2018
TI  - Integrin subunits alphaV and beta3 promote the osteogenic differentiation of umbilical 
      cord blood mesenchymal stem cells.
PG  - 2008-2016
AB  - Human umbilical cord-derived mesenchymal stem cells (hUCMSCs) are multipotent 
      cells that have self-renewal properties and can differentiate into osteocytes, 
      adipocytes, cartilage and extoderm. Bone regeneration and repair are important 
      for the repair of bone injury, skeletal development or continuous remodeling 
      throughout adult life. Thus, investigating the factors influencing osteocyte 
      regeneration from hUCMSCs could be conducive to advancements in skeletal repair 
      and the repair of bone injury. Previous reports have demonstrated that single 
      integrin subunits (alphaV, beta3, alpha5) and collagen I contribute to the osteogenic 
      differentiation of human mesenchymal stem cells (hMSCs). However, the functions 
      of the vitronectin receptor alphaV and beta3 in the osteogenic differentiation of 
      hUCMSCs and bone regeneration remain unclear. Run-related transcription factor 2 
      (RUNX2) is considered to be an early osteoblastic gene that is upregulated during 
      the osteogenic differentiation of hUCMSCs. Meanwhile, bone sialoprotein (BSP) and 
      collagen I are the most common early markers of osteoblast differentiation. 
      Herein, we found that the mRNA and protein expression of alphaV, beta3, RUNX2 and 
      collagen I were upregulated during the osteogenic differentiation of hUCMSCs. 
      Overexpression of alphaV and beta3 in hMSCs increased the levels of RUNX2, BSP, and 
      collagen I, decreased the number of adipocytes and promoted the osteogenic 
      differentiation of hUCMSCs. Meanwhile, downregulation of alphaV and beta3 decreased the 
      levels of RUNX2, BSP, and collagen I, increased the number of adipocytes and 
      blocked the osteogenic differentiation of hUCMSCs. In conclusion, the integrin 
      subunits alphaV and beta3 can promote the osteogenic differentiation of hUCMSCs and 
      encourage bone formation.
CI  - IJCEP Copyright (c) 2018.
FAU - Zheng, Hongyu
AU  - Zheng H
AD  - Department of Emergency, First Hospital Affiliated to Kunming Medical University 
      Kunming 650032, Yunnan, China.
FAU - Li, Xingguo
AU  - Li X
AD  - Department of Orthopedics, First Hospital Affiliated to Kunming Medical 
      University Kunming 650032, Yunnan, China.
FAU - Chen, Yuan
AU  - Chen Y
AD  - Department of Outpatient, First Hospital Affiliated to Kunming Medical University 
      Kunming 650032, Yunnan, China.
FAU - Zhou, Rudan
AU  - Zhou R
AD  - Department of Orthopedics, First Hospital Affiliated to Kunming Medical 
      University Kunming 650032, Yunnan, China.
FAU - Zhao, Hongbin
AU  - Zhao H
AD  - Department of Emergency, First Hospital Affiliated to Kunming Medical University 
      Kunming 650032, Yunnan, China.
FAU - Qian, Chuanyun
AU  - Qian C
AD  - Department of Emergency, First Hospital Affiliated to Kunming Medical University 
      Kunming 650032, Yunnan, China.
LA  - eng
PT  - Journal Article
DEP - 20180401
PL  - United States
TA  - Int J Clin Exp Pathol
JT  - International journal of clinical and experimental pathology
JID - 101480565
PMC - PMC6958194
OTO - NOTNLM
OT  - Human umbilical cord-derived mesenchymal stem cells (hUCMSCs)
OT  - RUNX2
OT  - collagen I
OT  - osteogenic differentiation
OT  - alphaV
OT  - beta3
COIS- None.
EDAT- 2018/04/01 00:00
MHDA- 2018/04/01 00:01
PMCR- 2018/04/01
CRDT- 2020/01/16 06:00
PHST- 2017/07/03 00:00 [received]
PHST- 2017/08/24 00:00 [accepted]
PHST- 2020/01/16 06:00 [entrez]
PHST- 2018/04/01 00:00 [pubmed]
PHST- 2018/04/01 00:01 [medline]
PHST- 2018/04/01 00:00 [pmc-release]
PST - epublish
SO  - Int J Clin Exp Pathol. 2018 Apr 1;11(4):2008-2016. eCollection 2018.