PMID- 31938576
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 2167-8359 (Print)
IS  - 2167-8359 (Electronic)
IS  - 2167-8359 (Linking)
VI  - 8
DP  - 2020
TI  - A pilot study on searching for peri-nuclear NeuN-positive cells.
PG  - e8254
LID - 10.7717/peerj.8254 [doi]
LID - e8254
AB  - The aim of this study was to find out neuron (-like) cells in peripheral organs 
      by cell markers in rats. Adult male Sprague-Dawley rats were anaesthetized. Their 
      organs including brain, heart, lung, liver, kidney, stomach, duodenum, and ileum 
      were harvested. The mRNA and protein in these organs were extracted. RNA 
      sequencing (RNA-Seq) was carried out, and NeuN, a "specific" marker for neuronal 
      soma, was assayed with Western blotting. The sections of the aforementioned 
      organs were obtained after a routine fixation (4% methanal)-dehydration 
      (ethanol)-embedding (paraffin) process. NeuN in the sections and seven 
      non-neuronal cell lines was analyzed by immunofluorescence (IF) or 
      immunohistochemistry (IHC). Neuronal markers, such as Eno2, NeuN (Rbfox3), 
      choline acetyltransferase (Chat), as well as tyrosine hydroxylase (Th), and 
      neuronal-glial markers, e.g., glial fibrillary acidic protein (Gfap), S100b, 2', 
      3'-cyclic nucleotide 3'-phosphodiesterase (Cnp), and other related markers, were 
      positively expressed in all the organs at mRNA level. NeuN was further analyzed 
      by Western blotting. The IF and IHC assays showed that NeuN-positive cells were 
      distributed in all the peripheral tissues (mainly peri-nuclear NeuN-positive 
      cells) though with different patterns from that in brain (nuclear NeuN-positive 
      cells), and a NeuN-negative tissue could not be found. Especially, NeuN and Myl3 
      co-expressed in the cytoplasm of myocardial cells, suggesting that NeuN could 
      possess other functions than neuronal differentiation. Also, the protein was 
      positively expressed in seven non-neuronal cell lines. Our findings suggested 
      that NeuN-positive cells exist widely, and without identification of its 
      distribution pattern, the specificity of NeuN for neurons could be limited.
CI  - (c)2020 Yu et al.
FAU - Yu, Yun
AU  - Yu Y
AD  - Department of Pharmacology, School of Basic Medicine, Kunming Medical University, 
      Kunming, Yunnan, China.
FAU - Wu, Meiyu
AU  - Wu M
AD  - Yunnan Provincial Key Laboratory of Molecular Biology for Sinomedicine, Faculty 
      of Basic Medicine, Yunnan University of Traditional Chinese Medicine, Kunming, 
      Yunnan, China.
FAU - Zhang, Nan
AU  - Zhang N
AD  - Department of Pharmacology, School of Basic Medicine, Kunming Medical University, 
      Kunming, Yunnan, China.
FAU - Yin, Hua
AU  - Yin H
AD  - Yunnan Provincial Key Laboratory of Molecular Biology for Sinomedicine, Faculty 
      of Basic Medicine, Yunnan University of Traditional Chinese Medicine, Kunming, 
      Yunnan, China.
FAU - Shu, Bin
AU  - Shu B
AD  - Jiangsu Center for Safety Evaluation of Drugs, Jiangsu Provincial Institute of 
      Materia Medica, Nanjing Tech University, Nanjing, Jiangsu, China.
FAU - Duan, Weigang
AU  - Duan W
AD  - Yunnan Provincial Key Laboratory of Molecular Biology for Sinomedicine, Faculty 
      of Basic Medicine, Yunnan University of Traditional Chinese Medicine, Kunming, 
      Yunnan, China.
LA  - eng
PT  - Journal Article
DEP - 20200107
PL  - United States
TA  - PeerJ
JT  - PeerJ
JID - 101603425
PMC - PMC6953339
OTO - NOTNLM
OT  - Fluorescence microscopy
OT  - High-throughput sequencing
OT  - Immunohistochemistry
OT  - NeuN protein
OT  - NeuN-positive cells
OT  - Western blotting
COIS- The authors declare there are no competing interests.
EDAT- 2020/01/16 06:00
MHDA- 2020/01/16 06:01
PMCR- 2020/01/07
CRDT- 2020/01/16 06:00
PHST- 2019/03/14 00:00 [received]
PHST- 2019/11/20 00:00 [accepted]
PHST- 2020/01/16 06:00 [entrez]
PHST- 2020/01/16 06:00 [pubmed]
PHST- 2020/01/16 06:01 [medline]
PHST- 2020/01/07 00:00 [pmc-release]
AID - 8254 [pii]
AID - 10.7717/peerj.8254 [doi]
PST - epublish
SO  - PeerJ. 2020 Jan 7;8:e8254. doi: 10.7717/peerj.8254. eCollection 2020.