PMID- 31939867
OWN - NLM
STAT- MEDLINE
DCOM- 20210618
LR  - 20210618
IS  - 1536-4801 (Electronic)
IS  - 0277-2116 (Linking)
VI  - 70
IP  - 4
DP  - 2020 Apr
TI  - Aberrant Distributions of Collagen I, III, and IV in Hirschsprung Disease.
PG  - 450-456
LID - 10.1097/MPG.0000000000002627 [doi]
AB  - BACKGROUND: Hirschsprung disease (HSCR) is the most common congenital gut 
      motility disorder, involving a severe anomaly of the enteric nervous system, and 
      is characterized by functional intestinal obstruction due to lack of intrinsic 
      innervation (aganglionosis) in the distal bowel. OBJECTIVE: The aim of this study 
      was to examine the distribution patterns of collagens I (Col I), III (Col III), 
      and IV (Col IV) in the enteric nervous system of HSCR patients, to determine 
      whether or not collagen levels are altered in the aganglionic bowel. METHODS: We 
      measured the expression levels of Col I, Col III, and Col IV in colonic muscle 
      from 129 children with HSCR. The localizations of the 3 collagens and myenteric 
      ganglia were assessed morphologically by immunofluorescence staining. western 
      blots and real-time fluorescence quantitative polymerase chain reaction were 
      performed to examine the relative levels of these collagens in aganglionic, 
      transitional, and ganglionic colon segments. RESULTS: Immunoreactivities of Col I 
      and Col III were high around and within myenteric ganglia in the ganglionic 
      segment, moderate in the transitional segment, and weak in the aganglionic 
      segment. Col IV immunoreactivity showed the opposite pattern, being lowest in the 
      ganglionic segment and highest in the aganglionic segment. CONCLUSION: Col I and 
      Col III are decreased and Col IV is increased in the distal colon of patients 
      with HSCR.
FAU - Gao, Ni
AU  - Gao N
AD  - Department of Pediatric Surgery, Qilu Hospital, Shandong University, Jinan, 
      Shandong Province, China.
FAU - Wang, Jian
AU  - Wang J
FAU - Zhang, Qiangye
AU  - Zhang Q
FAU - Zhou, Tingting
AU  - Zhou T
FAU - Mu, Weijing
AU  - Mu W
FAU - Hou, Peimin
AU  - Hou P
FAU - Wang, Dongming
AU  - Wang D
FAU - Lv, Xiaona
AU  - Lv X
FAU - Li, Aiwu
AU  - Li A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Pediatr Gastroenterol Nutr
JT  - Journal of pediatric gastroenterology and nutrition
JID - 8211545
SB  - IM
MH  - Blotting, Western
MH  - Child
MH  - Colon
MH  - *Hirschsprung Disease/genetics
MH  - Humans
MH  - Infant
MH  - Intestines
MH  - Real-Time Polymerase Chain Reaction
EDAT- 2020/01/16 06:00
MHDA- 2021/06/22 06:00
CRDT- 2020/01/16 06:00
PHST- 2020/01/16 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2020/01/16 06:00 [entrez]
AID - 00005176-202004000-00015 [pii]
AID - 10.1097/MPG.0000000000002627 [doi]
PST - ppublish
SO  - J Pediatr Gastroenterol Nutr. 2020 Apr;70(4):450-456. doi: 
      10.1097/MPG.0000000000002627.