PMID- 31940090
OWN - NLM
STAT- MEDLINE
DCOM- 20200402
LR  - 20200402
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 412
IP  - 5
DP  - 2020 Feb
TI  - Metabolomic profile perturbations of serum, lung, bronchoalveolar lavage fluid, 
      spleen and feces in LPS-induced acute lung injury rats based on HPLC-ESI-QTOF-MS.
PG  - 1215-1234
LID - 10.1007/s00216-019-02357-1 [doi]
AB  - Acute lung injury (ALI) is a clinically common and serious disease, underscoring 
      the urgent need for clarification of its pathogenesis. According to traditional 
      Chinese medicine (TCM) theories on the "lung-spleen-intestine axis" and its 
      correlation with ALI, a high-performance liquid chromatography-quadrupole 
      time-of-flight mass spectrometry (HPLC-QTOF-MS) metabolomic platform was applied 
      to identify biomarkers from five bio-samples of control and model rats challenged 
      with intratracheally administered lipopolysaccharide (LPS) based on multivariate 
      mathematical statistical analysis. As a result, 19, 24, 24, 15 and 29 altered 
      metabolites were identified in serum, lung, bronchoalveolar lavage fluid (BALF), 
      spleen and feces samples, respectively. Metabolic pathway analysis showed that 
      linoleic acid, sphingolipid, glycerophospholipid and bile acid metabolism 
      pathways were mainly altered by ALI. Additionally, ROC curves were applied to 
      assess the specificity and sensitivity of the biomarkers. ALI characteristic 
      metabolomic spectra were then established to differentiate the control from the 
      model group with a similarity discriminative threshold of 0.7. Additionally, to 
      compare the metabolomic profiles of the five bio-samples and establish metabolic 
      similarities and differences among them, correlation analysis was conducted in 
      order to delineate an objective law of endogenous linkage along the 
      lung-spleen-intestine axis. Therefore, this study provides insights into the 
      mechanisms involved in ALI from a metabolomics perspective, which can be applied 
      in characterization of the mechanism and early disease detection. Graphical 
      abstract.
FAU - Wang, Tianyang
AU  - Wang T
AD  - School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, 
      Shenyang, 110016, Liaoning, China.
FAU - Lin, Song
AU  - Lin S
AD  - Basic Medical Science College, Qiqihar Medical University, Qiqihar, 161006, 
      Heilongjiang, China.
FAU - Liu, Ran
AU  - Liu R
AD  - School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, 
      Shenyang, 110016, Liaoning, China.
FAU - Li, Hua
AU  - Li H
AD  - School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, 
      Shenyang, 110016, Liaoning, China.
FAU - Liu, Zihan
AU  - Liu Z
AD  - School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, 
      Shenyang, 110016, Liaoning, China.
FAU - Zhang, Xinnong
AU  - Zhang X
AD  - School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, 
      103 Wenhua Road, Shenyang, 110016, Liaoning, China.
FAU - Xu, Huarong
AU  - Xu H
AD  - School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, 
      Shenyang, 110016, Liaoning, China.
FAU - Li, Qing
AU  - Li Q
AD  - School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, 
      Shenyang, 110016, Liaoning, China.
FAU - Bi, Kaishun
AU  - Bi K
AD  - School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, 
      Shenyang, 110016, Liaoning, China. kaishunbi.syphu@gmail.com.
LA  - eng
GR  - for Qing Li (2017)/Liaoning Distinguished Professor Project/
GR  - Grant No.2018010961-301/Natural Science Foundation of Liaoning Province/
GR  - 2018226003/Key R&D Program of Liaoning Province/
GR  - U1508220/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20200115
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Biomarkers)
RN  - 0 (Cytokines)
RN  - 0 (Enzymes)
RN  - 0 (Lipopolysaccharides)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - Acute Lung Injury/blood/*metabolism
MH  - Animals
MH  - Biomarkers/blood
MH  - *Bronchoalveolar Lavage Fluid
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Cytokines/biosynthesis
MH  - Enzymes/metabolism
MH  - *Feces
MH  - Glutathione/metabolism
MH  - Lipopolysaccharides/*toxicity
MH  - Lung/*metabolism
MH  - Male
MH  - Malondialdehyde/metabolism
MH  - *Metabolomics
MH  - Rats
MH  - Rats, Wistar
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
MH  - Spleen/*metabolism
OTO - NOTNLM
OT  - Acute lung injury
OT  - Biomarker
OT  - HPLC-QTOF-MS
OT  - Metabolomics
EDAT- 2020/01/16 06:00
MHDA- 2020/04/03 06:00
CRDT- 2020/01/16 06:00
PHST- 2019/09/20 00:00 [received]
PHST- 2019/12/16 00:00 [accepted]
PHST- 2019/12/03 00:00 [revised]
PHST- 2020/01/16 06:00 [pubmed]
PHST- 2020/04/03 06:00 [medline]
PHST- 2020/01/16 06:00 [entrez]
AID - 10.1007/s00216-019-02357-1 [pii]
AID - 10.1007/s00216-019-02357-1 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2020 Feb;412(5):1215-1234. doi: 10.1007/s00216-019-02357-1. 
      Epub 2020 Jan 15.