PMID- 31940843
OWN - NLM
STAT- MEDLINE
DCOM- 20200603
LR  - 20200603
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 21
IP  - 2
DP  - 2020 Jan 11
TI  - Cannabinoid Receptor 2 Modulates Maturation of Dendritic Cells and Their Capacity 
      to Induce Hapten-Induced Contact Hypersensitivity.
LID - 10.3390/ijms21020475 [doi]
LID - 475
AB  - Contact hypersensitivity (CHS) is an established animal model for allergic 
      contact dermatitis. Dendritic cells (DCs) play an important role in the 
      sensitization phase of CHS by initiating T cell responses to topically applied 
      haptens. The cannabinoid receptors 1 (CB1) and 2 (CB2) modulate DC functions and 
      inflammatory skin responses, but their influence on the capacity of haptenized 
      DCs to induce CHS is still unknown. We found lower CHS responses to 
      2,4-dinitro-1-fluorobenzene (DNFB) in wild type (WT) mice after adoptive transfer 
      of haptenized Cnr2(-/-) and Cnr1(-/-)/Cnr2(-/-) bone marrow (BM) DCs as compared 
      to transfer of WT DCs. In contrast, induction of CHS was not affected in WT 
      recipients after transfer of Cnr1(-/-) DCs. In vitro stimulated Cnr2(-/-) DCs 
      showed lower CCR7 and CXCR4 expression when compared to WT cells, while in vitro 
      migration towards the chemokine ligands was not affected by CB2. Upregulation of 
      MHC class II and co-stimulatory molecules was also reduced in Cnr2(-/-) DCs. This 
      study demonstrates that CB2 modulates the maturation phenotype of DCs but not 
      their chemotactic capacities in vitro. These findings and the fact that CHS 
      responses mediated by Cnr2(-/-) DCs are reduced suggest that CB2 is a promising 
      target for the treatment of inflammatory skin conditions.
FAU - Gaffal, Evelyn
AU  - Gaffal E
AUID- ORCID: 0000-0002-4639-9841
AD  - Department of Dermatology, University Hospital Magdeburg, 39104 Magdeburg, 
      Germany.
FAU - Kemter, Andrea M
AU  - Kemter AM
AD  - Institute of Molecular Psychiatry, University of Bonn, 53127 Bonn, Germany.
AD  - Department of Pathology, The University of Chicago, Chicago, IL 60637, USA.
FAU - Scheu, Stefanie
AU  - Scheu S
AUID- ORCID: 0000-0002-9707-8191
AD  - Institute of Medical Microbiology and Hospital Hygiene, University of Dusseldorf, 
      40225 Dusseldorf, Germany.
FAU - Leite Dantas, Rafael
AU  - Leite Dantas R
AUID- ORCID: 0000-0001-7358-990X
AD  - Department of Psychiatry, University of Munster, 48149 Munster, Germany.
FAU - Vogt, Jens
AU  - Vogt J
AD  - Department of Psychiatry, University of Munster, 48149 Munster, Germany.
FAU - Baune, Bernhard
AU  - Baune B
AD  - Department of Psychiatry, University of Munster, 48149 Munster, Germany.
AD  - Department of Psychiatry, The University of Melbourne, Melbourne 3010, Australia.
AD  - The Florey Institute of Neuroscience and Mental Health, The University of 
      Melbourne, Melbourne 3010, Australia.
FAU - Tuting, Thomas
AU  - Tuting T
AD  - Department of Dermatology, University Hospital Magdeburg, 39104 Magdeburg, 
      Germany.
FAU - Zimmer, Andreas
AU  - Zimmer A
AD  - Institute of Molecular Psychiatry, University of Bonn, 53127 Bonn, Germany.
FAU - Alferink, Judith
AU  - Alferink J
AD  - Department of Psychiatry, University of Munster, 48149 Munster, Germany.
AD  - Cells in Motion Interfaculty Centre, 48149 Muenster, Germany.
LA  - eng
GR  - DFG-FOR926 to JA, EG and AZ/Deutsche Forschungsgemeinschaft/
GR  - DFG EXC 1003; Grant FF-2014-01 Cells in Motion-Cluster of Excellence to 
      JA/Deutsche Forschungsgemeinschaft/
GR  - SCHE692/3-1, SCHE692/4-1 to SS/Deutsche Forschungsgemeinschaft/
GR  - DFG EXC 1023 Cluster of Excellence ImmunoSensation (EXC 1023) to AZ/Deutsche 
      Forschungsgemeinschaft/
GR  - Alf3/018/16 to JA/IZKF Munster/
GR  - I-ST111423 and I-DL121204 to JA/IMF (University Munster)/
GR  - Strategic Research Fund of the Heinrich Heine University Dusseldorf to 
      SS/Strategic Research Fund of the Heinrich Heine University Dusseldorf/
PT  - Journal Article
DEP - 20200111
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (CNR1 protein, mouse)
RN  - 0 (Ccr4 protein, mouse)
RN  - 0 (Ccr7 protein, mouse)
RN  - 0 (Cnr2 protein, mouse)
RN  - 0 (Histocompatibility Antigens)
RN  - 0 (Receptor, Cannabinoid, CB1)
RN  - 0 (Receptor, Cannabinoid, CB2)
RN  - 0 (Receptors, CCR4)
RN  - 0 (Receptors, CCR7)
RN  - D241E059U6 (Dinitrofluorobenzene)
SB  - IM
MH  - Animals
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Chemotaxis
MH  - Dendritic Cells/cytology/*immunology
MH  - Dermatitis, Allergic Contact/genetics/*immunology
MH  - Dinitrofluorobenzene/toxicity
MH  - Histocompatibility Antigens/genetics/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Receptor, Cannabinoid, CB1/genetics/metabolism
MH  - Receptor, Cannabinoid, CB2/genetics/*metabolism
MH  - Receptors, CCR4/genetics/metabolism
MH  - Receptors, CCR7/genetics/metabolism
PMC - PMC7013892
OTO - NOTNLM
OT  - CB1
OT  - CB2
OT  - CCL19
OT  - CXCL12
OT  - MHC
OT  - allergic contact dermatitis
OT  - cannabinoid receptors
OT  - dendritic cells
OT  - hapten 2,4-dinitro-1-fluorobenzene
OT  - migration
OT  - skin inflammation
COIS- The authors declare no conflict of interest.
EDAT- 2020/01/17 06:00
MHDA- 2020/06/04 06:00
PMCR- 2020/01/01
CRDT- 2020/01/17 06:00
PHST- 2019/11/30 00:00 [received]
PHST- 2020/01/03 00:00 [revised]
PHST- 2020/01/07 00:00 [accepted]
PHST- 2020/01/17 06:00 [entrez]
PHST- 2020/01/17 06:00 [pubmed]
PHST- 2020/06/04 06:00 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - ijms21020475 [pii]
AID - ijms-21-00475 [pii]
AID - 10.3390/ijms21020475 [doi]
PST - epublish
SO  - Int J Mol Sci. 2020 Jan 11;21(2):475. doi: 10.3390/ijms21020475.