PMID- 31940925 OWN - NLM STAT- MEDLINE DCOM- 20201111 LR - 20201111 IS - 2072-6643 (Electronic) IS - 2072-6643 (Linking) VI - 12 IP - 1 DP - 2020 Jan 12 TI - Identification of a Circulating Amino Acid Signature in Frail Older Persons with Type 2 Diabetes Mellitus: Results from the Metabofrail Study. LID - 10.3390/nu12010199 [doi] LID - 199 AB - Diabetes and frailty are highly prevalent conditions that impact the health status of older adults. Perturbations in protein/amino acid metabolism are associated with both functional impairment and type 2 diabetes mellitus (T2DM). In the present study, we compared the concentrations of a panel of circulating 37 amino acids and derivatives between frail/pre-frail older adults with T2DM and robust non-diabetic controls. Sixty-six functionally impaired older persons aged 70+ with T2DM and 30 age and sex-matched controls were included in the analysis. We applied a partial least squares-discriminant analysis (PLS-DA)-based analytical strategy to characterize the metabotype of study participants. The optimal complexity of the PLS-DA model was found to be two latent variables. The proportion of correct classification was 94.1 +/- 1.9% for frail/pre-frail persons with T2DM and 100% for control participants. Functionally impaired older persons with T2DM showed higher levels of 3-methyl histidine, alanine, arginine, glutamic acid, ethanolamine sarcosine, and tryptophan. Control participants had higher levels of ornithine and taurine. These findings indicate that a specific profile of amino acids and derivatives characterizes pre-frail/frail older persons with T2DM. The dissection of these pathways may provide novel insights into the metabolic perturbations involved in the disabling cascade in older persons with T2DM. FAU - Calvani, Riccardo AU - Calvani R AUID- ORCID: 0000-0001-5472-2365 AD - Universita Cattolica del Sacro Cuore, 00168 Rome, Italy. AD - Fondazione Policlinico Universitario "Agostino Gemelli" IRCCS, 00168 Rome, Italy. FAU - Rodriguez-Manas, Leocadio AU - Rodriguez-Manas L AD - Servicio de Geriatria, Hospital Universitario de Getafe, 28905 Madrid, Spain. FAU - Picca, Anna AU - Picca A AUID- ORCID: 0000-0001-7032-3487 AD - Universita Cattolica del Sacro Cuore, 00168 Rome, Italy. AD - Fondazione Policlinico Universitario "Agostino Gemelli" IRCCS, 00168 Rome, Italy. FAU - Marini, Federico AU - Marini F AUID- ORCID: 0000-0001-8266-1117 AD - Department of Chemistry, Sapienza Universita di Roma, 00185 Rome, Italy. FAU - Biancolillo, Alessandra AU - Biancolillo A AD - Department of Physical and Chemical Sciences, Universita degli Studi dell'Aquila, 67100 L'Aquila, Italy. FAU - Laosa, Olga AU - Laosa O AD - Foundation for Biomedical Research, Hospital Universitario de Getafe, 28905 Madrid, Spain. FAU - Pedraza, Laura AU - Pedraza L AD - Foundation for Biomedical Research, Hospital Universitario de Getafe, 28905 Madrid, Spain. FAU - Gervasoni, Jacopo AU - Gervasoni J AD - Universita Cattolica del Sacro Cuore, 00168 Rome, Italy. AD - Fondazione Policlinico Universitario "Agostino Gemelli" IRCCS, 00168 Rome, Italy. FAU - Primiano, Aniello AU - Primiano A AD - Universita Cattolica del Sacro Cuore, 00168 Rome, Italy. AD - Fondazione Policlinico Universitario "Agostino Gemelli" IRCCS, 00168 Rome, Italy. FAU - Conta, Giorgia AU - Conta G AD - Department of Chemistry, Sapienza Universita di Roma, 00185 Rome, Italy. FAU - Bourdel-Marchasson, Isabelle AU - Bourdel-Marchasson I AD - Centre Hospitalier Universitaire de Bordeaux, 33000 Bordeaux, France. FAU - Regueme, Sophie C AU - Regueme SC AD - Centre Hospitalier Universitaire de Bordeaux, 33000 Bordeaux, France. FAU - Bernabei, Roberto AU - Bernabei R AD - Universita Cattolica del Sacro Cuore, 00168 Rome, Italy. AD - Fondazione Policlinico Universitario "Agostino Gemelli" IRCCS, 00168 Rome, Italy. FAU - Marzetti, Emanuele AU - Marzetti E AUID- ORCID: 0000-0001-9567-6983 AD - Universita Cattolica del Sacro Cuore, 00168 Rome, Italy. AD - Fondazione Policlinico Universitario "Agostino Gemelli" IRCCS, 00168 Rome, Italy. FAU - Sinclair, Alan J AU - Sinclair AJ AD - Foundation for Diabetes Research in Older People, Diabetes Frail Ltd., Luton LU1 3UA, UK. FAU - Gambassi, Giovanni AU - Gambassi G AD - Universita Cattolica del Sacro Cuore, 00168 Rome, Italy. AD - Fondazione Policlinico Universitario "Agostino Gemelli" IRCCS, 00168 Rome, Italy. LA - eng GR - 278803/Seventh Framework Programme/ GR - 115621/Innovative Medicines Initiative/ GR - N/A/Centro Studi Achille e Linda Lorenzon/ PT - Evaluation Study PT - Journal Article DEP - 20200112 PL - Switzerland TA - Nutrients JT - Nutrients JID - 101521595 RN - 0 (Amino Acids, Cyclic) RN - 0 (Biomarkers) SB - IM MH - Aged MH - Aged, 80 and over MH - Amino Acids, Cyclic/*blood MH - Biomarkers/blood MH - Case-Control Studies MH - Diabetes Mellitus, Type 2/*blood/complications MH - Female MH - Frailty/*blood/complications MH - Humans MH - Least-Squares Analysis MH - Male MH - Metabolome MH - Randomized Controlled Trials as Topic PMC - PMC7019630 OTO - NOTNLM OT - aging OT - frailty OT - metabolic profiling OT - metabolism OT - metabolomics OT - muscle wasting OT - personalized medicine OT - precision medicine OT - sarcopenia OT - systems biology COIS- A.J.S., E.M., L.R.-M., R.B., and R.C are partners of the SPRINTT Consortium, which is partly funded by the European Federation of Pharmaceutical Industries and Associations (EFPIA). R.C. served as a consultant for Abbott, Novartis, and Nutricia. E.M. served as a consultant for Abbott, Biophytis, Nestle, Novartis, and Nutricia. EDAT- 2020/01/17 06:00 MHDA- 2020/11/12 06:00 PMCR- 2020/01/01 CRDT- 2020/01/17 06:00 PHST- 2019/12/20 00:00 [received] PHST- 2020/01/08 00:00 [revised] PHST- 2020/01/09 00:00 [accepted] PHST- 2020/01/17 06:00 [entrez] PHST- 2020/01/17 06:00 [pubmed] PHST- 2020/11/12 06:00 [medline] PHST- 2020/01/01 00:00 [pmc-release] AID - nu12010199 [pii] AID - nutrients-12-00199 [pii] AID - 10.3390/nu12010199 [doi] PST - epublish SO - Nutrients. 2020 Jan 12;12(1):199. doi: 10.3390/nu12010199.