PMID- 31941423
OWN - NLM
STAT- MEDLINE
DCOM- 20210907
LR  - 20210907
IS  - 1557-8577 (Electronic)
IS  - 1549-1684 (Linking)
VI  - 23
IP  - 5
DP  - 2020 Oct
TI  - Zengye Decoction Ameliorates Insulin Resistance by Promoting Glucose Uptake.
PG  - 367-376
LID - 10.1089/rej.2019.2228 [doi]
AB  - The incidence of type 2 diabetes mellitus (T2DM) has been increasing in recent 
      years and has become a serious threat to human health. Zengye Decoction (ZYD), a 
      well-known traditional Chinese medicinal formula, has been used in the treatment 
      of T2DM with yin asthenia and extreme heat since Qing Dynasty. However, the 
      characteristics of antidiabetic activities of ZYD have not been fully elucidated. 
      In our study, high-fat diet and streptozotocin were used to establish the T2DM 
      rat model. After 3 weeks of treatment with ZYD, the fasting blood glucose (FBG), 
      oral glucose tolerance, the fasting serum insulin concentration, insulin 
      sensitivity index (ISI), serum lipid profiles, and pancreas histopathology were 
      measured. Then, under circumstance of insulin-resistant glucose consumption, 
      2-(N-(7-nitrobenz-2-oxa-l,3-diazol-4-yl) amino)-2-deoxyglucose (2-NBDG) uptake 
      and glycogen content in C2C12 myotubes, 3T3-L1 adipocytes, and HepG2 cells were 
      determined, respectively. Finally, the expressions of key targets in the insulin 
      signaling pathway were measured to explain the potential mechanism underlying 
      these activities. After administration with ZYD, a notable reduction in FBG 
      levels, oral glucose tolerance test-area under the curve, blood lipid metabolism, 
      and ISI values were observed compared with the diabetic control group. Moreover, 
      ZYD restored the damaged islet cells in T2DM rats. Significant increases in 
      glucose consumption, glucose uptake, glycogen content, expression of glucose 
      transporter type 4, and the ratio of p-Akt/Akt were observed in the ZYD groups. 
      According to the above results, ZYD exhibited glucose disposal, including glucose 
      consumption, glucose uptake, and glycogen content and promoted the Akt signal 
      pathway, which indicates that ZYD exerts significant hypoglycemic effect in T2DM.
FAU - Wang, Mei
AU  - Wang M
AD  - Jiangsu Key Laboratory of TCM Evaluation and Translational Research, School of 
      Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, China.
FAU - Chang, Shan-Quan
AU  - Chang SQ
AD  - Jiangsu Key Laboratory of TCM Evaluation and Translational Research, School of 
      Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, China.
FAU - Tian, Yu-Shan
AU  - Tian YS
AD  - Jiangsu Key Laboratory of TCM Evaluation and Translational Research, School of 
      Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, China.
FAU - Zhang, Ge-Qiang
AU  - Zhang GQ
AD  - School of Pharmacy, China Pharmaceutical University, Nanjing, China.
FAU - Qi, Jin
AU  - Qi J
AD  - Jiangsu Key Laboratory of TCM Evaluation and Translational Research, School of 
      Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, China.
AD  - State Key Laboratory of Natural Medicines, School of Traditional Chinese 
      Pharmacy, China Pharmaceutical University, Nanjing, China.
LA  - eng
PT  - Journal Article
DEP - 20200415
PL  - United States
TA  - Rejuvenation Res
JT  - Rejuvenation research
JID - 101213381
RN  - 0 (Blood Glucose)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Insulin)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Blood Glucose
MH  - *Diabetes Mellitus, Type 2/drug therapy
MH  - *Drugs, Chinese Herbal/therapeutic use
MH  - Glucose
MH  - Insulin
MH  - *Insulin Resistance
MH  - Rats
OTO - NOTNLM
OT  - Akt
OT  - GLUT4
OT  - Zengye Decoction
OT  - antidiabetic
OT  - glucose metabolism
EDAT- 2020/01/17 06:00
MHDA- 2021/09/08 06:00
CRDT- 2020/01/17 06:00
PHST- 2020/01/17 06:00 [pubmed]
PHST- 2021/09/08 06:00 [medline]
PHST- 2020/01/17 06:00 [entrez]
AID - 10.1089/rej.2019.2228 [doi]
PST - ppublish
SO  - Rejuvenation Res. 2020 Oct;23(5):367-376. doi: 10.1089/rej.2019.2228. Epub 2020 
      Apr 15.