PMID- 31941952
OWN - NLM
STAT- MEDLINE
DCOM- 20201106
LR  - 20210114
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 10
IP  - 1
DP  - 2020 Jan 15
TI  - Immunophenotyping monocytes, macrophages and granulocytes in the Pteropodid bat 
      Eonycteris spelaea.
PG  - 309
LID - 10.1038/s41598-019-57212-1 [doi]
LID - 309
AB  - Bats are asymptomatic reservoir hosts for several highly pathogenic viruses. 
      Understanding this enigmatic relationship between bats and emerging zoonotic 
      viruses requires tools and approaches which enable the comparative study of bat 
      immune cell populations and their functions. We show that bat genomes have a 
      conservation of immune marker genes which delineate phagocyte populations in 
      humans, while lacking key mouse surface markers such as Ly6C and Ly6G. 
      Cross-reactive antibodies against CD44, CD11b, CD14, MHC II, and CD206 were 
      multiplexed to characterize circulating monocytes, granulocytes, bone-marrow 
      derived macrophages (BMDMs) and lung alveolar macrophages (AMs) in the cave 
      nectar bat Eonycteris spelaea. Transcriptional profiling of bat monocytes and 
      BMDMs identified additional markers - including MARCO, CD68, CD163, CD172alpha, and 
      CD88 - which can be used to further characterize bat myeloid populations. Bat 
      cells often resembled their human counterparts when comparing immune parameters 
      that are divergent between humans and mice, such as the expression patterns of 
      certain immune cell markers. A genome-wide comparison of immune-related genes 
      also revealed a much closer phylogenetic relationship between bats and humans 
      compared to rodents. Taken together, this study provides a set of tools and a 
      comparative framework which will be important for unravelling viral disease 
      tolerance mechanisms in bats.
FAU - Gamage, Akshamal M
AU  - Gamage AM
AD  - Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, 
      Singapore.
FAU - Zhu, Feng
AU  - Zhu F
AD  - Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, 
      Singapore.
FAU - Ahn, Matae
AU  - Ahn M
AD  - Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, 
      Singapore.
FAU - Foo, Randy Jee Hiang
AU  - Foo RJH
AD  - Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, 
      Singapore.
FAU - Hey, Ying Ying
AU  - Hey YY
AD  - Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, 
      Singapore.
FAU - Low, Dolyce H W
AU  - Low DHW
AD  - Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, 
      Singapore.
FAU - Mendenhall, Ian H
AU  - Mendenhall IH
AD  - Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, 
      Singapore.
FAU - Dutertre, Charles-Antoine
AU  - Dutertre CA
AD  - Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, 
      Singapore.
AD  - Singapore Immunology Network (SIgN), Agency for Science Technology and Research 
      (A*STAR), Singapore, Singapore.
FAU - Wang, Lin-Fa
AU  - Wang LF
AUID- ORCID: 0000-0003-2752-0535
AD  - Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, 
      Singapore. linfa.wang@duke-nus.edu.sg.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200115
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CD163 antigen)
RN  - 0 (Interleukin-8)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Antigens, CD/metabolism
MH  - Antigens, Differentiation, Myelomonocytic/metabolism
MH  - Cell Differentiation/drug effects
MH  - Chiroptera/classification/genetics/*immunology/virology
MH  - Gene Expression/drug effects
MH  - Genome
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Granulocytes/cytology/*metabolism
MH  - Humans
MH  - Immunophenotyping/*methods
MH  - Interleukin-8/genetics/metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - Macrophages/cytology/drug effects/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Monocytes/cytology/*metabolism
MH  - Phylogeny
MH  - Receptors, Cell Surface/metabolism
MH  - Tumor Necrosis Factor-alpha/genetics/metabolism
PMC - PMC6962400
COIS- The authors declare no competing interests.
EDAT- 2020/01/17 06:00
MHDA- 2020/11/11 06:00
PMCR- 2020/01/15
CRDT- 2020/01/17 06:00
PHST- 2019/05/17 00:00 [received]
PHST- 2019/12/12 00:00 [accepted]
PHST- 2020/01/17 06:00 [entrez]
PHST- 2020/01/17 06:00 [pubmed]
PHST- 2020/11/11 06:00 [medline]
PHST- 2020/01/15 00:00 [pmc-release]
AID - 10.1038/s41598-019-57212-1 [pii]
AID - 57212 [pii]
AID - 10.1038/s41598-019-57212-1 [doi]
PST - epublish
SO  - Sci Rep. 2020 Jan 15;10(1):309. doi: 10.1038/s41598-019-57212-1.