PMID- 31942024
OWN - NLM
STAT- MEDLINE
DCOM- 20201117
LR  - 20210114
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 10
IP  - 1
DP  - 2020 Jan 15
TI  - Peptide hormone sensors using human hormone receptor-carrying nanovesicles and 
      graphene FETs.
PG  - 388
LID - 10.1038/s41598-019-57339-1 [doi]
LID - 388
AB  - Hormones within very low levels regulate and control the activity of specific 
      cells and organs of the human body. Hormone imbalance can cause many diseases. 
      Therefore, hormone detection tools have been developed, particularly over the 
      last decade. Peptide hormones have a short half-life, so it is important to 
      detect them within a short time. In this study, we report two types of peptide 
      hormone sensors using human hormone receptor-carrying nanovesicles and graphene 
      field-effect transistors (FETs). Parathyroid hormone (PTH) and glucagon (GCG) are 
      peptide hormones present in human blood that act as ligands to G protein-coupled 
      receptors (GPCRs). In this paper, the parathyroid hormone receptor (PTHR) and the 
      glucagon receptor (GCGR) were expressed in human embryonic kidney-293 (HEK-293) 
      cells, and were constructed as nanovesicles carrying the respective receptors. 
      They were then immobilized onto graphene-based FETs. The two hormone sensors 
      developed were able to detect each target hormone with high sensitivity (ca. 100 
      fM of PTH and 1 pM of GCG). Also, the sensors accurately recognized target 
      hormones among different types of peptide hormones. In the development of hormone 
      detection tools, this approach, using human hormone receptor-carrying 
      nanovesicles and graphene FETs, offers the possibility of detecting very low 
      concentrations of hormones in real-time.
FAU - Ahn, Sae Ryun
AU  - Ahn SR
AD  - School of Chemical and Biological Engineering, Seoul National University, Seoul, 
      08826, Republic of Korea.
FAU - An, Ji Hyun
AU  - An JH
AD  - School of Chemical and Biological Engineering, Seoul National University, Seoul, 
      08826, Republic of Korea.
AD  - Semiconductor R&D Center, Samsung Electronics, Hwaseong, Gyeonggi, 18448, 
      Republic of Korea.
FAU - Lee, Seung Hwan
AU  - Lee SH
AD  - School of Chemical and Biological Engineering, Seoul National University, Seoul, 
      08826, Republic of Korea.
AD  - Department of Bionano Engineering, Hanyang University, Ansan, 15588, Republic of 
      Korea.
FAU - Song, Hyun Seok
AU  - Song HS
AD  - Sensor System Research Center, Korea Institute of Science and Technology, Seoul, 
      02792, Republic of Korea.
FAU - Jang, Jyongsik
AU  - Jang J
AD  - School of Chemical and Biological Engineering, Seoul National University, Seoul, 
      08826, Republic of Korea. jsjang@plaza.snu.ac.kr.
FAU - Park, Tai Hyun
AU  - Park TH
AD  - School of Chemical and Biological Engineering, Seoul National University, Seoul, 
      08826, Republic of Korea. thpark@snu.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200115
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Peptide Hormones)
RN  - 0 (Receptors, Peptide)
RN  - 7782-42-5 (Graphite)
SB  - IM
MH  - Biosensing Techniques/*methods
MH  - Graphite/*chemistry
MH  - HEK293 Cells
MH  - Humans
MH  - Nanoparticles/*chemistry
MH  - Peptide Hormones/*analysis/metabolism
MH  - Receptors, Peptide/chemistry/*metabolism
MH  - *Transistors, Electronic
PMC - PMC6962399
COIS- The authors declare no competing interests.
EDAT- 2020/01/17 06:00
MHDA- 2020/11/18 06:00
PMCR- 2020/01/15
CRDT- 2020/01/17 06:00
PHST- 2019/06/04 00:00 [received]
PHST- 2019/12/19 00:00 [accepted]
PHST- 2020/01/17 06:00 [entrez]
PHST- 2020/01/17 06:00 [pubmed]
PHST- 2020/11/18 06:00 [medline]
PHST- 2020/01/15 00:00 [pmc-release]
AID - 10.1038/s41598-019-57339-1 [pii]
AID - 57339 [pii]
AID - 10.1038/s41598-019-57339-1 [doi]
PST - epublish
SO  - Sci Rep. 2020 Jan 15;10(1):388. doi: 10.1038/s41598-019-57339-1.