PMID- 31943822
OWN - NLM
STAT- MEDLINE
DCOM- 20200722
LR  - 20220716
IS  - 2326-5205 (Electronic)
IS  - 2326-5191 (Linking)
VI  - 72
IP  - 6
DP  - 2020 Jun
TI  - DNA Vaccination With Hsp70 Protects Against Systemic Lupus Erythematosus in (NZB 
      x NZW)F1 Mice.
PG  - 997-1002
LID - 10.1002/art.41202 [doi]
AB  - OBJECTIVE: To address whether a targeted modulation of the abnormal expression of 
      Hsp70 and autoantibodies against this molecule in systemic lupus erythematosus 
      can influence disease. METHODS: Lupus-prone (NZB x NZW)F1 mice that had been 
      DNA-vaccinated with plasmids encoding Hsp70 and controls were monitored for lupus 
      disease parameters including anti-double stranded DNA (anti-dsDNA) autoantibodies 
      and cytokines using enzyme-linked immunosorbent assay, and for kidney function 
      and pathology. The phenotypic and numerical changes in relevant immune cells were 
      evaluated by flow cytometry, and cell function was assessed. RESULTS: Mice that 
      had been DNA-vaccinated with Hsp70 displayed marked suppression of anti-dsDNA 
      antibody production, reduced renal disease, and antiinflammatory responses that 
      are associated with a significantly extended survival, compared to controls. 
      These protective effects in Hsp70-vaccinated mice were associated with an 
      induction of tolerogenic immune responses and an expansion of functional Treg 
      cells. CONCLUSION: DNA vaccination with Hsp70 suppresses murine lupus by inducing 
      tolerogenic immune responses and antiinflammatory immune responses associated 
      with reduced disease manifestations and increased mouse survival.
CI  - (c) 2020, American College of Rheumatology.
FAU - Liu, Aijing
AU  - Liu A
AD  - University of California, Los Angeles.
FAU - Ferretti, Concetta
AU  - Ferretti C
AD  - University of California, Los Angeles.
FAU - Shi, Fu-Dong
AU  - Shi FD
AD  - Barrow Neurological Institute, Phoenix, Arizona.
FAU - Cohen, Irun R
AU  - Cohen IR
AD  - The Weizmann Institute of Science, Rehovot, Israel.
FAU - Quintana, Francisco J
AU  - Quintana FJ
AD  - Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts.
FAU - La Cava, Antonio
AU  - La Cava A
AUID- ORCID: 0000-0002-8839-0933
AD  - University of California, Los Angeles.
LA  - eng
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20200430
PL  - United States
TA  - Arthritis Rheumatol
JT  - Arthritis & rheumatology (Hoboken, N.J.)
JID - 101623795
RN  - 0 (Antibodies, Antinuclear)
RN  - 0 (Autoantibodies)
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (Vaccines, DNA)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Antibodies, Antinuclear/*drug effects/immunology
MH  - Autoantibodies/*drug effects/immunology
MH  - DNA/immunology
MH  - Disease Models, Animal
MH  - Enzyme-Linked Immunosorbent Assay
MH  - HSP70 Heat-Shock Proteins/*pharmacology
MH  - Lupus Erythematosus, Systemic/immunology/*prevention & control
MH  - Mice
MH  - Mice, Inbred NZB
MH  - Vaccines, DNA/*pharmacology
EDAT- 2020/01/17 06:00
MHDA- 2020/07/23 06:00
CRDT- 2020/01/17 06:00
PHST- 2019/07/15 00:00 [received]
PHST- 2020/01/07 00:00 [accepted]
PHST- 2020/01/17 06:00 [pubmed]
PHST- 2020/07/23 06:00 [medline]
PHST- 2020/01/17 06:00 [entrez]
AID - 10.1002/art.41202 [doi]
PST - ppublish
SO  - Arthritis Rheumatol. 2020 Jun;72(6):997-1002. doi: 10.1002/art.41202. Epub 2020 
      Apr 30.