PMID- 31947510
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 2076-2607 (Print)
IS  - 2076-2607 (Electronic)
IS  - 2076-2607 (Linking)
VI  - 8
IP  - 1
DP  - 2020 Jan 14
TI  - Immunobiotic and Paraprobiotic Potential Effect of Lactobacillus casei in a 
      Systemic Toxoplasmosis Murine Model.
LID - 10.3390/microorganisms8010113 [doi]
LID - 113
AB  - One of the main characteristics of probiotics is their ability to stimulate and 
      modulate the immune response regardless of their viability. Lactobacillus casei 
      (Lc) can stimulate local and systemic immunity, in addition to the activation of 
      macrophages at sites distant from the intestine. Activated macrophages limit the 
      replication of intracellular protozoa, such as Toxoplasma gondii, through the 
      production of nitric oxide. The present study aimed to evaluate the protection 
      generated by treatment with viable and non-viable Lc in the murine systemic 
      toxoplasmosis model. CD1 male mice were treated with viable Lc (immunobiotic) and 
      non-viable Lc (paraprobiotic), infected with tachyzoites of Toxoplasma gondii RH 
      strain. The reduction of the parasitic load, activation of peritoneal 
      macrophages, inflammatory cytokines, and cell populations was evaluated at 7 days 
      post-infection, in addition to the survival. The immunobiotic and paraprobiotic 
      reduced the parasitic load, but only the immunobiotic increased the activation of 
      peritoneal macrophages, and the production of interferon-gamma (IFN-gamma), tumor 
      necrosis factor (TNF), and interleukin-6 (IL-6) while the paraprobiotic increased 
      the production of monocyte chemoattractant protein-1 (MCP-1) and T CD4(+)CD44(+) 
      lymphocytes. Viable and non-viable Lc increases survival but does not prevent the 
      death of animals. The results provide evidence about the remote immunological 
      stimulation of viable and non-viable Lc in an in vivo parasitic model.
FAU - Salas-Lais, Angel Gustavo
AU  - Salas-Lais AG
AD  - Departamento de Microbiologia y Proteomica Ocular de la Unidad de Investigacion 
      del Instituto de Oftalmologia "Fundacion de Asistencia Privada Conde de 
      Valenciana I.A.P.", Chimalpopoca 14, Obrera, Ciudad de Mexico 06800, Mexico.
AD  - Laboratorio de Tecnicas Fototermicas, Departamento de Ciencias Basicas, Unidad 
      Profesional Interdisciplinaria de Biotecnologia del Instituto Politecnico 
      Nacional, Acueducto 550, La Laguna Ticoman, Ciudad de Mexico 07340, Mexico.
AD  - Programa de Posgrado en Biomedicina y Biotecnologia Molecular, Escuela Nacional 
      de Ciencias Biologicas, Instituto Politecnico Nacional, Mexico City 11340, 
      Mexico.
FAU - Robles-Contreras, Atzin
AU  - Robles-Contreras A
AUID- ORCID: 0000-0002-8393-3556
AD  - Area de Imunoproteomica Ocular, Departamento Centro de Investigacion Biomedica, 
      Fundacion Hospital "Nuestra Senora de la Luz", Ezequiel Montes 135, Tabacalera, 
      Ciudad de Mexico 06030, Mexico.
FAU - Balderas-Lopez, Jose Abraham
AU  - Balderas-Lopez JA
AD  - Laboratorio de Tecnicas Fototermicas, Departamento de Ciencias Basicas, Unidad 
      Profesional Interdisciplinaria de Biotecnologia del Instituto Politecnico 
      Nacional, Acueducto 550, La Laguna Ticoman, Ciudad de Mexico 07340, Mexico.
FAU - Bautista-de Lucio, Victor Manuel
AU  - Bautista-de Lucio VM
AUID- ORCID: 0000-0003-1836-8738
AD  - Departamento de Microbiologia y Proteomica Ocular de la Unidad de Investigacion 
      del Instituto de Oftalmologia "Fundacion de Asistencia Privada Conde de 
      Valenciana I.A.P.", Chimalpopoca 14, Obrera, Ciudad de Mexico 06800, Mexico.
LA  - eng
PT  - Journal Article
DEP - 20200114
PL  - Switzerland
TA  - Microorganisms
JT  - Microorganisms
JID - 101625893
PMC - PMC7023318
OTO - NOTNLM
OT  - Lactobacillus casei
OT  - activated macrophages
OT  - immunobiotic
OT  - paraprobiotic
OT  - systemic toxoplasmosis
OT  - tachyzoites
COIS- The authors declare no conflict of interest.
EDAT- 2020/01/18 06:00
MHDA- 2020/01/18 06:01
PMCR- 2020/01/14
CRDT- 2020/01/18 06:00
PHST- 2019/12/01 00:00 [received]
PHST- 2020/01/07 00:00 [revised]
PHST- 2020/01/08 00:00 [accepted]
PHST- 2020/01/18 06:00 [entrez]
PHST- 2020/01/18 06:00 [pubmed]
PHST- 2020/01/18 06:01 [medline]
PHST- 2020/01/14 00:00 [pmc-release]
AID - microorganisms8010113 [pii]
AID - microorganisms-08-00113 [pii]
AID - 10.3390/microorganisms8010113 [doi]
PST - epublish
SO  - Microorganisms. 2020 Jan 14;8(1):113. doi: 10.3390/microorganisms8010113.