PMID- 31948543
OWN - NLM
STAT- MEDLINE
DCOM- 20210104
LR  - 20210104
IS  - 1557-7988 (Electronic)
IS  - 0076-6879 (Linking)
VI  - 631
DP  - 2020
TI  - Cytokine profiling of tumor-infiltrating T lymphocytes by flow cytometry.
PG  - 1-20
LID - S0076-6879(19)30360-X [pii]
LID - 10.1016/bs.mie.2019.08.015 [doi]
AB  - Intracellular cytokine staining (ICS) utilizing fluorescently labeled, 
      cytokine-specific antibodies is a powerful technique utilized to evaluate 
      cytokine expression that provides resolution at the single cell level. Combined 
      with multi-parameter flow cytometry, ICS can provide detailed information on 
      complex cytokine profiles and cellular phenotypes within the tumor 
      microenvironment, particularly for the CD4(+) T helper and CD8(+) cytotoxic T 
      cell compartments. This technique provides critical information concerning 
      tumor-infiltrating T cell function that is essential for evaluating existing or 
      therapeutically-induced tumor antigen-specific T cell responses in both 
      preclinical models and cancer patients. In this chapter we will discuss in detail 
      the critical steps necessary for a successful ICS assay and outline common 
      protocols for the evaluation of cytokine production from T cell subsets present 
      within the tumor microenvironment.
CI  - (c) 2020 Elsevier Inc. All rights reserved.
FAU - Foote, Jeremy B
AU  - Foote JB
AD  - Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL, 
      United States. Electronic address: jbf130@uab.edu.
FAU - Sarvesh, Sujith
AU  - Sarvesh S
AD  - Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL, 
      United States.
FAU - Emens, Leisha A
AU  - Emens LA
AD  - Department of Medicine, UPMC Hillman Cancer Center, and UPMC Magee Women's 
      Hospital, University of Pittsburgh, Pittsburgh, PA, United States. Electronic 
      address: emensla@upmc.edu.
LA  - eng
PT  - Journal Article
DEP - 20191018
PL  - United States
TA  - Methods Enzymol
JT  - Methods in enzymology
JID - 0212271
RN  - 0 (Cytokines)
SB  - IM
MH  - Animals
MH  - Cytokines/*analysis
MH  - Flow Cytometry/*methods
MH  - Humans
MH  - Immunologic Techniques/*methods
MH  - Lymphocytes, Tumor-Infiltrating/*metabolism
MH  - T-Lymphocytes/metabolism
MH  - Tumor Microenvironment/*immunology
OTO - NOTNLM
OT  - Cancer
OT  - Intracellular cytokine staining
OT  - Tumor-infiltrating lymphocytes
EDAT- 2020/01/18 06:00
MHDA- 2021/01/05 06:00
CRDT- 2020/01/18 06:00
PHST- 2020/01/18 06:00 [entrez]
PHST- 2020/01/18 06:00 [pubmed]
PHST- 2021/01/05 06:00 [medline]
AID - S0076-6879(19)30360-X [pii]
AID - 10.1016/bs.mie.2019.08.015 [doi]
PST - ppublish
SO  - Methods Enzymol. 2020;631:1-20. doi: 10.1016/bs.mie.2019.08.015. Epub 2019 Oct 
      18.