PMID- 31948856
OWN - NLM
STAT- MEDLINE
DCOM- 20210809
LR  - 20210809
IS  - 2530-0180 (Electronic)
IS  - 2530-0180 (Linking)
VI  - 67
IP  - 7
DP  - 2020 Aug-Sep
TI  - Evaluation of miR-181b and miR-126-5p expression levels in T2DM patients compared 
      to healthy individuals: Relationship with NF-kappaB gene expression.
PG  - 454-460
LID - S2530-0164(19)30252-6 [pii]
LID - 10.1016/j.endinu.2019.09.009 [doi]
AB  - BACKGROUND: Type 2 diabetes mellitus (T2DM) is a progressive metabolic disorder 
      whose prevalence is rising very fast across the world. Diagnosis of this disease 
      in early stages (pre-diabetic stage) plays an important role in reducing 
      mortality associated with this disorder. miRNAs, as key players in the 
      pathogenesis of T2DM, have been investigated in several studies. Furthermore, 
      their expression profile changes in the early stages of diabetes mellitus in body 
      fluids such as serum, peripheral blood, and peripheral blood mononuclear cell 
      (PBMC) have been studied. Due to their high stability and the presence of 
      non-invasive sensitive methods for their measurement, such as real-time PCR, they 
      can be used for early diagnosis of T2DM as a biomarker. In this experimental 
      study, the expression levels of miR-181b, miR-126-5p, and NF-kappaB were measured in 
      patients with T2DM, pre-diabetic subjects, and healthy controls in a Yazd 
      population. MATERIAL AND METHOD: Ninety asymptomatic subjects including 30 T2DM, 
      30 pre-diabetic, and 30 healthy subjects (diagnosis based on WHO criteria) were 
      included in this study. Real-time PCR was used to measure the expression levels 
      of miR-181b and miR-126-5p. Moreover, the NF-kappaB expression level was also 
      measured to determine its relationship with these two microRNAs. RESULT: In this 
      study, the expression level of miR-181b and miR-126-p decreased gradually in 
      pre-diabetic as well as T2DM subjects compared to healthy controls. Furthermore, 
      our study showed a significant negative correlation between these two miRNAs and 
      NF-kappaB for the first time. CONCLUSION: These results introduce these 
      anti-inflammatory miRNAs as powerful tools for early diagnosis of T2DM.
CI  - Copyright (c) 2019 SEEN y SED. Publicado por Elsevier Espana, S.L.U. All rights 
      reserved.
FAU - Dehghani, Mohammadreza
AU  - Dehghani M
AD  - Medical Genetics Research Centre, Shahid Sadoughi University of Medical Sciences, 
      Yazd, Iran.
FAU - Aghaei Zarch, Seyed Mohsen
AU  - Aghaei Zarch SM
AD  - Department of Medical Genetics, School of Medicine, Shahid Sadoughi University of 
      Medical Science, Yazd, Iran. Electronic address: mosenaghaei123@gmail.com.
FAU - Vahidi Mehrjardi, Mohammad Yahya
AU  - Vahidi Mehrjardi MY
AD  - Medical Genetics Research Centre, Shahid Sadoughi University of Medical Sciences, 
      Yazd, Iran.
FAU - Nazari, Majid
AU  - Nazari M
AD  - Department of Medical Genetics, School of Medicine, Shahid Sadoughi University of 
      Medical Science, Yazd, Iran.
FAU - Babakhanzadeh, Emad
AU  - Babakhanzadeh E
AD  - Department of Medical Genetics, School of Medicine, Shahid Sadoughi University of 
      Medical Science, Yazd, Iran.
FAU - Ghadimi, Hamidreza
AU  - Ghadimi H
AD  - Department of Genetics, Tehran University of Medical Sciences, Tehran, Iran.
FAU - Zeinali, Fahime
AU  - Zeinali F
AD  - Department of Nutrition, Shahid Sadoughi University of Medical Science, Yazd, 
      Iran.
FAU - Talebi, Mehrdad
AU  - Talebi M
AD  - Department of Medical Genetics, School of Medicine, Shahid Sadoughi University of 
      Medical Science, Yazd, Iran.
LA  - eng
LA  - spa
PT  - Comparative Study
PT  - Journal Article
DEP - 20200113
PL  - Spain
TA  - Endocrinol Diabetes Nutr (Engl Ed)
JT  - Endocrinologia, diabetes y nutricion
JID - 101717565
RN  - 0 (MIRN126 microRNA, human)
RN  - 0 (MIrn181 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (NF-kappa B)
SB  - IM
MH  - Diabetes Mellitus, Type 2/*genetics
MH  - Female
MH  - *Gene Expression
MH  - Humans
MH  - Male
MH  - MicroRNAs/*genetics
MH  - Middle Aged
MH  - NF-kappa B/*genetics
OTO - NOTNLM
OT  - Diabetes tipo 2
OT  - NF-kappaB
OT  - Type 2 diabetes mellitus
OT  - miR-126-5p
OT  - miR-181b
OT  - mir-126-5p
OT  - mir-181b
EDAT- 2020/01/18 06:00
MHDA- 2021/08/10 06:00
CRDT- 2020/01/18 06:00
PHST- 2019/04/30 00:00 [received]
PHST- 2019/09/09 00:00 [revised]
PHST- 2019/09/10 00:00 [accepted]
PHST- 2020/01/18 06:00 [pubmed]
PHST- 2021/08/10 06:00 [medline]
PHST- 2020/01/18 06:00 [entrez]
AID - S2530-0164(19)30252-6 [pii]
AID - 10.1016/j.endinu.2019.09.009 [doi]
PST - ppublish
SO  - Endocrinol Diabetes Nutr (Engl Ed). 2020 Aug-Sep;67(7):454-460. doi: 
      10.1016/j.endinu.2019.09.009. Epub 2020 Jan 13.