PMID- 31949614
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200121
IS  - 1936-2625 (Electronic)
IS  - 1936-2625 (Linking)
VI  - 11
IP  - 11
DP  - 2018
TI  - miR-375 is downregulated by promoter hypermethylation in MIN6 insulinoma cells.
PG  - 5335-5342
AB  - Epigenetics may affect the susceptibility for type 2 diabetes mellitus (T2DM). 
      Aberrant DNA methylation patterns are nowadays recognized as a key epigenetic 
      hallmark of T2DM. Previously, our studies have shown that the hypomethylation of 
      human miR-375 promoter may contribute to the pathogenesis of T2DM. However, no 
      comprehensive study defines the miR-375 promoter methylation patterns present in 
      the established pancreatic beta cell line. To address this matter, we have analyzed 
      the DNA methylation profile of insulinoma MIN6 cells by MassARRAY spectrometry 
      and employed the DNA demethylating drug 5-aza-2'-deoxycytidine (5-aza-CdR) to 
      treat MIN6 cells to explore the methylation patterns of the mmu-miR-375. The 
      expression of mmu-miR-375 in mRNA level was measured by quantitative RT-PCR 
      (qRT-PCR). Methylation analysis reveals that MIN6 cells display hypermethylation 
      at the mmu-miR-375 promoter. Following the decreased methylation of mmu-miR-375, 
      the relative expression of mmu-miR-375 increased gradually after 5-Aza-CdR 
      treatment. In addition, we find that there was an inverse correlation between DNA 
      methylation levels and transcription level of mmu-miR-375. In summary, this is 
      the first report for analyzing mmu-miR-375 promoter methylation using MALDI-TOF 
      MS technology and our results indicate that promoter hypermethylation of the 
      mmu-miR-375 is a common event in MIN6 cells.
CI  - IJCEP Copyright (c) 2018.
FAU - Wei, Yu
AU  - Wei Y
AD  - Department of Clinical Laboratory, The First Affiliated Hospital of Medical 
      College of Shihezi University Shihezi 832002, Xinjiang, P. R. China.
FAU - Cai, Wei-Juan
AU  - Cai WJ
AD  - Department of Clinical Laboratory, The First Affiliated Hospital of Medical 
      College of Shihezi University Shihezi 832002, Xinjiang, P. R. China.
FAU - Kuang, Xue-Mei
AU  - Kuang XM
AD  - Department of Endocrinology and Metabolism, Tongji Hospital, Tongji Medical 
      College, Huazhong University of Science and Technology Wuhan 43000, Hubei, P. R. 
      China.
FAU - Yin, Liang
AU  - Yin L
AD  - Department of Endocrinology and Metabolism, Tongji Hospital, Tongji Medical 
      College, Huazhong University of Science and Technology Wuhan 43000, Hubei, P. R. 
      China.
LA  - eng
PT  - Journal Article
DEP - 20181101
PL  - United States
TA  - Int J Clin Exp Pathol
JT  - International journal of clinical and experimental pathology
JID - 101480565
PMC - PMC6963046
OTO - NOTNLM
OT  - 5-aza-2'-deoxycytidine
OT  - DNA methylation
OT  - Type 2 diabetes mellitus
OT  - insulinoma MIN6 cells
COIS- None.
EDAT- 2018/11/01 00:00
MHDA- 2018/11/01 00:01
PMCR- 2018/11/01
CRDT- 2020/01/18 06:00
PHST- 2018/08/21 00:00 [received]
PHST- 2018/09/25 00:00 [accepted]
PHST- 2020/01/18 06:00 [entrez]
PHST- 2018/11/01 00:00 [pubmed]
PHST- 2018/11/01 00:01 [medline]
PHST- 2018/11/01 00:00 [pmc-release]
PST - epublish
SO  - Int J Clin Exp Pathol. 2018 Nov 1;11(11):5335-5342. eCollection 2018.