PMID- 31951623
OWN - NLM
STAT- MEDLINE
DCOM- 20200410
LR  - 20200410
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 15
IP  - 1
DP  - 2020
TI  - Molecular karyotyping of Siberian wild rye (Elymus sibiricus L.) with 
      oligonucleotide fluorescence in situ hybridization (FISH) probes.
PG  - e0227208
LID - 10.1371/journal.pone.0227208 [doi]
LID - e0227208
AB  - Siberian wild rye (Elymus sibiricus L.), an allotetraploid species, is a 
      potentially high-quality perennial forage crop native to temperate regions. We 
      used fluorescently conjugated oligonucleotides, representing ten repetitive 
      sequences, including 6 microsatellite repeats, two satellite repeats, and two 
      ribosomal DNAs, to characterize E. sibiricus chromosomes, using sequential 
      fluorescence in situ hybridization and genomic in situ hybridization assays. Our 
      results showed that microsatellite repeats (AAG)10 or (AGG)10, satellite repeats 
      pAs1 and pSc119.2, and ribosomal 5S rDNA and 45S rDNA are specific markers for 
      unique chromosomes. A referable karyotype ideogram was suggested, by further 
      polymorphism screening, across different E. sibiricus cultivars with a probe 
      mixture of (AAG)10, Oligo-pAs1, and Oligo-pSc119.2. Chromosomal polymorphisms 
      vary between different genomes and between different individual chromosomes. In 
      particular, two distinct forms of chromosome E in H genome were identified in 
      intra- and inter-populations. Here, the significance of these results, for E. 
      sibiricus genome research and breeding, and novel approaches to improve 
      fluorescence in situ hybridization-based karyotyping are discussed.
FAU - Xie, Jihong
AU  - Xie J
AD  - Grassland Research Institute, Chinese Academy of Agricultural Sciences, Hohhot, 
      China.
FAU - Zhao, Yan
AU  - Zhao Y
AD  - College of Grassland, Resource and Environmental Science, Inner Mongolia 
      Agricultural University, Hohhot, China.
FAU - Yu, Linqing
AU  - Yu L
AD  - Grassland Research Institute, Chinese Academy of Agricultural Sciences, Hohhot, 
      China.
FAU - Liu, Ruijuan
AU  - Liu R
AD  - Key Laboratory of Crop Molecular Breeding, Qinghai Province, Northwest Institute 
      of Plateau Biology, Chinese Academy of Sciences, Xining, China.
AD  - Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Plateau 
      Institute of Biology, Chinese Academy of Sciences, Xining, China.
FAU - Dou, Quanwen
AU  - Dou Q
AUID- ORCID: 0000-0003-0945-5039
AD  - Key Laboratory of Crop Molecular Breeding, Qinghai Province, Northwest Institute 
      of Plateau Biology, Chinese Academy of Sciences, Xining, China.
AD  - Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Plateau 
      Institute of Biology, Chinese Academy of Sciences, Xining, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200117
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (DNA, Plant)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Oligonucleotide Probes)
SB  - IM
MH  - Chromosomes, Plant
MH  - DNA, Plant/genetics
MH  - DNA, Ribosomal/genetics
MH  - Elymus/*genetics
MH  - *Genome, Plant
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Microsatellite Repeats
MH  - Oligonucleotide Probes/genetics
PMC - PMC6968859
COIS- The authors have declared that no competing interests exist.
EDAT- 2020/01/18 06:00
MHDA- 2020/04/11 06:00
PMCR- 2020/01/17
CRDT- 2020/01/18 06:00
PHST- 2019/07/16 00:00 [received]
PHST- 2019/12/14 00:00 [accepted]
PHST- 2020/01/18 06:00 [entrez]
PHST- 2020/01/18 06:00 [pubmed]
PHST- 2020/04/11 06:00 [medline]
PHST- 2020/01/17 00:00 [pmc-release]
AID - PONE-D-19-17005 [pii]
AID - 10.1371/journal.pone.0227208 [doi]
PST - epublish
SO  - PLoS One. 2020 Jan 17;15(1):e0227208. doi: 10.1371/journal.pone.0227208. 
      eCollection 2020.