PMID- 31955175
OWN - NLM
STAT- MEDLINE
DCOM- 20210203
LR  - 20231012
IS  - 1423-0313 (Electronic)
IS  - 0031-7012 (Linking)
VI  - 105
IP  - 5-6
DP  - 2020
TI  - Scopoletin Activates Adenosine Monophosphate-Activated Protein Kinase/Mammalian 
      Target of Rapamycin Signaling Pathway and Improves Functional Recovery after 
      Spinal Cord Injury in Rats.
PG  - 349-359
LID - 10.1159/000503866 [doi]
AB  - BACKGROUND: Scopoletin (SPT) is known to exert neuroprotective autophagy effect. 
      However, the efficacy of SPT in the treatment of spinal cord injury (SCI) has yet 
      not been explored. The investigation was intended to elucidate whether SPT can 
      exert neuroprotective effect by triggering neuronal autophagy after SCI. The 
      study was also directed to investigate the role of adenosine 
      monophosphate-activated protein kinase (AMPK)/mammalian target of rapamycin 
      (mTOR) signaling pathway in the autophagy facilitated by SPT. MATERIALS AND 
      METHODS: The SCI was developed in female Sprague-Dawley rats by damaging the T10 
      spinal level using an impounder impact. Three animals groups were investigated - 
      Sham group, SCI group, and SCI + SPT group. The SCI + SPT group was administered 
      with SPT (100 mg/kg) intraperitoneally. Basso, Beattie, and Bresnahan scores and 
      angle of incline test revealed that SPT administration improved the movement of 
      hind limbs after SCI induction. RESULTS: Results indicated that SPT imparted 
      neuronal protection, alleviated neuronal apoptosis, and improved neuronal 
      autophagy. SPT-induced autophagy was identified by increased Beclin-1 expression 
      and LC3B-positive neuronal cells. Further investigations revealed that SPT 
      triggers the pathway involving AMPK/mTOR signaling, thereby stimulating autophagy 
      in SCI-induced rat model. CONCLUSION: The findings of the present investigation 
      strongly advocate the beneficial effects of SPT in the treatment of the SCI. SPT 
      ameliorates the AMPK/mTOR signaling-induced autophagy and thereby improves 
      functional recovery in SCI-induced rats.
CI  - (c) 2020 S. Karger AG, Basel.
FAU - Zhou, Ruijuan
AU  - Zhou R
AD  - Department of Rehabilitation, Shanghai General Hospital, Shanghai Jiaotong 
      University, Shanghai, China.
FAU - Kan, Shifeng
AU  - Kan S
AD  - Department of Rehabilitation, Shanghai General Hospital, Shanghai Jiaotong 
      University, Shanghai, China.
FAU - Cai, Song
AU  - Cai S
AD  - Department of Rehabilitation, Shanghai General Hospital, Shanghai Jiaotong 
      University, Shanghai, China.
FAU - Sun, Ran
AU  - Sun R
AD  - Department of Rehabilitation, Shanghai General Hospital, Shanghai Jiaotong 
      University, Shanghai, China.
FAU - Yuan, Haixin
AU  - Yuan H
AD  - Department of Rehabilitation, Shanghai Fifth Rehabilitation Hospital, Shanghai, 
      China.
FAU - Yu, Bo
AU  - Yu B
AD  - Department of Rehabilitation, Shanghai General Hospital, Shanghai Jiaotong 
      University, Shanghai, China, blainedavidsonchu@yahoo.com.
LA  - eng
PT  - Journal Article
PT  - Retracted Publication
DEP - 20200117
PL  - Switzerland
TA  - Pharmacology
JT  - Pharmacology
JID - 0152016
RN  - 0 (Neuroprotective Agents)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
RN  - KLF1HS0SXJ (Scopoletin)
SB  - IM
RIN - Pharmacology. 2024;109(1):65. PMID: 37827136
MH  - AMP-Activated Protein Kinases/*metabolism
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Autophagy/drug effects
MH  - Female
MH  - Locomotion/drug effects
MH  - Neurons/drug effects
MH  - Neuroprotective Agents/*pharmacology/therapeutic use
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Recovery of Function/drug effects
MH  - Scopoletin/*pharmacology/therapeutic use
MH  - Signal Transduction/drug effects
MH  - Spinal Cord/drug effects
MH  - Spinal Cord Injuries/*drug therapy/metabolism/pathology/physiopathology
MH  - TOR Serine-Threonine Kinases/*metabolism
OTO - NOTNLM
OT  - Autophagy
OT  - Neuroprotective
OT  - Scopoletin
OT  - Spinal cord injury
EDAT- 2020/01/20 06:00
MHDA- 2021/02/04 06:00
CRDT- 2020/01/20 06:00
PHST- 2019/08/20 00:00 [received]
PHST- 2019/10/01 00:00 [accepted]
PHST- 2020/01/20 06:00 [pubmed]
PHST- 2021/02/04 06:00 [medline]
PHST- 2020/01/20 06:00 [entrez]
AID - 000503866 [pii]
AID - 10.1159/000503866 [doi]
PST - ppublish
SO  - Pharmacology. 2020;105(5-6):349-359. doi: 10.1159/000503866. Epub 2020 Jan 17.