PMID- 31955909
OWN - NLM
STAT- MEDLINE
DCOM- 20210111
LR  - 20210111
IS  - 1618-0372 (Electronic)
IS  - 0065-1281 (Linking)
VI  - 122
IP  - 3
DP  - 2020 Apr
TI  - Differences in the expression of catecholamine-synthesizing enzymes between 
      vesicular monoamine transporter 1- and 2-immunoreactive glomus cells in the rat 
      carotid body.
PG  - 151507
LID - S0065-1281(20)30006-4 [pii]
LID - 10.1016/j.acthis.2020.151507 [doi]
AB  - Vesicular monoamine transporters (VMAT) 1 and 2 are responsible for monoamine 
      transportation into secretary vesicles and are tissue-specifically expressed in 
      central and peripheral monoaminergic tissues, including the carotid body (CB). 
      The aim of the present study was to examine the expression of 
      catecholamine-synthesizing enzymes in VMAT1- and VMAT2-immunoreactive glomus 
      cells in the rat CB using multiple immunolabeling. The expression of VMAT1 and 
      VMAT2 mRNA in the CB was confirmed by RT-PCR. Immunohistochemistry revealed that 
      VMAT1 immunoreactivity was predominant in glomus cells rather than VMAT2 
      immunoreactivity. Glomus cells with VMAT1 immunoreactivity exhibited 
      weak/negative VMAT2 immunoreactivity, and vice versa. Immunoreactivities for 
      VMAT1 and tyrosine hydroxylase, the rate-limiting enzyme for catecholamine 
      biosynthesis, were co-localized in the same glomus cells and a positive 
      correlation was confirmed between the two immunoreactivities (Spearman's 
      coefficient = 0.82; p <  0.05). Although some glomus cells showed co-localization 
      of VMAT2 and dopamine beta-hydroxylase immunoreactivity, the biosynthetic enzyme for 
      noradrenaline, VMAT2 immunoreactivity appeared to be less associated with both 
      catecholamine-synthesizing enzymes as indicated by a correlation analysis (TH: 
      Spearman's coefficient = 0.38, DBH: Spearman's coefficient = 0.26). These results 
      indicate that heterogeneity on functional role would exist among glomus cells in 
      terms of VMAT isoform and catecholamine-synthesizing enzymes expression.
CI  - Copyright (c) 2020 Elsevier GmbH. All rights reserved.
FAU - Kato, Kouki
AU  - Kato K
AD  - Center for Laboratory Animal Science, National Defense Medical College, 3-2 
      Namiki, Tokorozawa, Saitama 359-8513, Japan.
FAU - Yokoyama, Takuya
AU  - Yokoyama T
AD  - Department of Anatomy (Cell Biology), Iwate Medical University, 2-1-1 
      Nishitokuta, Yahaba, Iwate 028-3694, Japan.
FAU - Kusakabe, Tatsumi
AU  - Kusakabe T
AD  - Laboratory for Anatomy and Physiology, Department of Sport and Medical Science, 
      Kokushikan University, 7-3-1 Nagayama, Tama, Tokyo 206-8515, Japan.
FAU - Hata, Katsuhiko
AU  - Hata K
AD  - Laboratory for Anatomy and Physiology, Department of Sport and Medical Science, 
      Kokushikan University, 7-3-1 Nagayama, Tama, Tokyo 206-8515, Japan.
FAU - Fushuku, Seigo
AU  - Fushuku S
AD  - Center for Laboratory Animal Science, National Defense Medical College, 3-2 
      Namiki, Tokorozawa, Saitama 359-8513, Japan.
FAU - Nakamuta, Nobuaki
AU  - Nakamuta N
AD  - Laboratory of Veterinary Anatomy and Cell Biology, Faculty of Agriculture, Iwate 
      University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan.
FAU - Yamamoto, Yoshio
AU  - Yamamoto Y
AD  - Laboratory of Veterinary Anatomy and Cell Biology, Faculty of Agriculture, Iwate 
      University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan. Electronic address: 
      yyoshio@iwate-u.ac.jp.
LA  - eng
PT  - Journal Article
DEP - 20200116
PL  - Germany
TA  - Acta Histochem
JT  - Acta histochemica
JID - 0370320
RN  - 0 (Catecholamines)
RN  - 0 (RNA, Messenger)
RN  - 0 (Slc18a1 protein, rat)
RN  - 0 (Slc18a2 protein, rat)
RN  - 0 (Vesicular Monoamine Transport Proteins)
RN  - EC 1.14.17.1 (Dopamine beta-Hydroxylase)
RN  - X4W3ENH1CV (Norepinephrine)
SB  - IM
MH  - Animals
MH  - Carotid Body/cytology/*metabolism
MH  - Catecholamines/*biosynthesis
MH  - Dopamine beta-Hydroxylase/metabolism
MH  - Immunohistochemistry
MH  - Male
MH  - Norepinephrine/biosynthesis
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Rats
MH  - Rats, Wistar
MH  - Vesicular Monoamine Transport Proteins/genetics/*metabolism
OTO - NOTNLM
OT  - Carotid body
OT  - Dopamine
OT  - Glomus cell
OT  - Immunohistochemistry
OT  - Noradrenaline
OT  - Vesicular monoamine transporter
COIS- Declaration of Competing Interest The authors declare that they have no conflict 
      of interest.
EDAT- 2020/01/21 06:00
MHDA- 2021/01/12 06:00
CRDT- 2020/01/21 06:00
PHST- 2019/09/08 00:00 [received]
PHST- 2019/12/06 00:00 [revised]
PHST- 2019/12/27 00:00 [accepted]
PHST- 2020/01/21 06:00 [pubmed]
PHST- 2021/01/12 06:00 [medline]
PHST- 2020/01/21 06:00 [entrez]
AID - S0065-1281(20)30006-4 [pii]
AID - 10.1016/j.acthis.2020.151507 [doi]
PST - ppublish
SO  - Acta Histochem. 2020 Apr;122(3):151507. doi: 10.1016/j.acthis.2020.151507. Epub 
      2020 Jan 16.