PMID- 31957813
OWN - NLM
STAT- MEDLINE
DCOM- 20201105
LR  - 20201105
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 24
IP  - 1
DP  - 2020 Jan
TI  - MiR-7a ameliorates spinal cord injury by inhibiting neuronal apoptosis and 
      oxidative stress.
PG  - 11-17
LID - 19890 [pii]
LID - 10.26355/eurrev_202001_19890 [doi]
AB  - OBJECTIVE: The aim of this study was to investigate the therapeutic effect of 
      microRNA-7a (miR-7a) on spinal cord injured rats and to explore its underlying 
      mechanism in vivo. MATERIALS AND METHODS: The spinal cord injury (SCI) model was 
      first established in adult rats. The epicenter of the lesion was treated with 
      miR-7a mimics via intrathecal injection. The Basso-Beattie-Bresnahan (BBB) 
      locomotor rating scale was used to evaluate the functional recovery of hindlimbs 
      in rats within 4 weeks following SCI. Western blotting and qPCR were utilized to 
      detect the apoptosis and oxidative stress in rats treated with or without miR-7a. 
      In addition, the neuron survival and neuro-filament amount were determined using 
      immunofluorescence. RESULTS: After SCI and miR-7a treatment, the locomotor 
      recovery of treated rats was significantly improved when compared with rats 
      without treatment. The mitochondrial disorder and cell death were significantly 
      reduced in miR-7a treated rats. Meanwhile, the nuclear transcription factor-kappaB 
      (NF-kappaB) pathway was significantly reduced as well. Contrarily, the expression of 
      anti-apoptotic protein Bcl-2 and NF-kappaB inhibitor I-kappaB was remarkably elevated in 
      miR-7a treated rats. In addition, up-regulation of miR-7a rescued neurons and 
      maintained the neural structure. CONCLUSIONS: The up-regulation of miR-7a 
      alleviated the injury-induced oxidative stress and inhibited apoptosis by 
      down-regulating NF-kappaB pathway in SCI rats.
FAU - Ding, L-Z
AU  - Ding LZ
AD  - Department of Orthopedics, Taizhou Central Hospital, Taizhou, China. 
      Gui625625625@163.com.
FAU - Xu, J
AU  - Xu J
FAU - Yuan, C
AU  - Yuan C
FAU - Teng, X
AU  - Teng X
FAU - Wu, Q-M
AU  - Wu QM
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (MIRN7-1 microRNA, rat)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Disease Models, Animal
MH  - Male
MH  - MicroRNAs/genetics/*metabolism
MH  - Neurons/*metabolism/pathology
MH  - Oxidative Stress
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Spinal Cord Injuries/*metabolism/pathology
EDAT- 2020/01/21 06:00
MHDA- 2020/11/06 06:00
CRDT- 2020/01/21 06:00
PHST- 2020/01/21 06:00 [entrez]
PHST- 2020/01/21 06:00 [pubmed]
PHST- 2020/11/06 06:00 [medline]
AID - 19890 [pii]
AID - 10.26355/eurrev_202001_19890 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2020 Jan;24(1):11-17. doi: 
      10.26355/eurrev_202001_19890.