PMID- 31958206
OWN - NLM
STAT- MEDLINE
DCOM- 20210603
LR  - 20240329
IS  - 1439-7633 (Electronic)
IS  - 1439-4227 (Print)
IS  - 1439-4227 (Linking)
VI  - 21
IP  - 12
DP  - 2020 Jun 15
TI  - Chimeric Interaction of Nitrogenase-Like Reductases with the MoFe Protein of 
      Nitrogenase.
PG  - 1733-1741
LID - 10.1002/cbic.201900759 [doi]
AB  - The engineering of transgenic organisms with the ability to fix nitrogen is an 
      attractive possibility. However, oxygen sensitivity of nitrogenase, mainly 
      conferred by the reductase component (NifH)(2) , is an imminent problem. 
      Nitrogenase-like enzymes involved in coenzyme F(430) and chlorophyll biosynthesis 
      utilize the highly homologous reductases (CfbC)(2) and (ChlL)(2) , respectively. 
      Chimeric protein-protein interactions of these reductases with the catalytic 
      component of nitrogenase (MoFe protein) did not support nitrogenase activity. 
      Nucleotide-dependent association and dissociation of these complexes was 
      investigated, but (CfbC)(2) and wild-type (ChlL)(2) showed no modulation of the 
      binding affinity. By contrast, the interaction between the (ChlL)(2) mutant Y127S 
      and the MoFe protein was markedly increased in the presence of ATP (or ATP 
      analogues) and reduced in the ADP state. Upon formation of the octameric 
      (ChlL)(2) MoFe(ChlL)(2) complex, the ATPase activity of this variant is 
      triggered, as seen in the homologous nitrogenase system. Thus, the described 
      reductase(s) might be an attractive tool for further elucidation of the diverse 
      functions of (NifH)(2) and the rational design of a more robust reductase.
CI  - (c) 2020 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.
FAU - Jasper, Jan
AU  - Jasper J
AUID- ORCID: 0000-0003-3300-1993
AD  - Institut fur Mikrobiologie, Technische Universitat Braunschweig, Spielmannstrasse 
      7, 38106, Braunschweig, Germany.
FAU - Ramos, Jose V
AU  - Ramos JV
AD  - Institut fur Pharmazeutische Wissenschaften, Pharmazeutische Biologie und 
      Biotechnologie, Albert-Ludwigs-Universitat Freiburg, Stefan-Meier-Str. 19, 79104, 
      Freiburg, Germany.
FAU - Trncik, Christian
AU  - Trncik C
AD  - Institut fur Biochemie, Albert-Ludwigs-Universitat Freiburg, Albertstrasse 21, 
      79104, Freiburg, Germany.
FAU - Jahn, Dieter
AU  - Jahn D
AUID- ORCID: 0000-0002-4064-9205
AD  - Institut fur Mikrobiologie, Technische Universitat Braunschweig, Spielmannstrasse 
      7, 38106, Braunschweig, Germany.
FAU - Einsle, Oliver
AU  - Einsle O
AUID- ORCID: 0000-0001-8722-2893
AD  - Institut fur Biochemie, Albert-Ludwigs-Universitat Freiburg, Albertstrasse 21, 
      79104, Freiburg, Germany.
FAU - Layer, Gunhild
AU  - Layer G
AUID- ORCID: 0000-0001-5927-1671
AD  - Institut fur Pharmazeutische Wissenschaften, Pharmazeutische Biologie und 
      Biotechnologie, Albert-Ludwigs-Universitat Freiburg, Stefan-Meier-Str. 19, 79104, 
      Freiburg, Germany.
FAU - Moser, Jurgen
AU  - Moser J
AUID- ORCID: 0000-0001-9373-4967
AD  - Institut fur Mikrobiologie, Technische Universitat Braunschweig, Spielmannstrasse 
      7, 38106, Braunschweig, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200227
PL  - Germany
TA  - Chembiochem
JT  - Chembiochem : a European journal of chemical biology
JID - 100937360
RN  - 0 (Molybdoferredoxin)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.18.6.1 (Nitrogenase)
SB  - IM
MH  - Methanosarcina barkeri/*enzymology
MH  - Molecular Structure
MH  - Molybdoferredoxin/*chemistry/metabolism
MH  - Nitrogenase/*chemistry/metabolism
MH  - Oxidoreductases/*chemistry/metabolism
MH  - Protein Binding
PMC - PMC7317204
OTO - NOTNLM
OT  - enzyme catalysis
OT  - metalloproteins
OT  - nitrogen fixation
OT  - nitrogenases
OT  - protein-protein interactions
COIS- The authors declare no conflict of interest.
EDAT- 2020/01/21 06:00
MHDA- 2021/06/04 06:00
PMCR- 2020/06/26
CRDT- 2020/01/21 06:00
PHST- 2019/12/16 00:00 [received]
PHST- 2020/01/17 00:00 [revised]
PHST- 2020/01/21 06:00 [pubmed]
PHST- 2021/06/04 06:00 [medline]
PHST- 2020/01/21 06:00 [entrez]
PHST- 2020/06/26 00:00 [pmc-release]
AID - CBIC201900759 [pii]
AID - 10.1002/cbic.201900759 [doi]
PST - ppublish
SO  - Chembiochem. 2020 Jun 15;21(12):1733-1741. doi: 10.1002/cbic.201900759. Epub 2020 
      Feb 27.