PMID- 31960744
OWN - NLM
STAT- MEDLINE
DCOM- 20201201
LR  - 20220413
IS  - 1724-6008 (Electronic)
IS  - 0393-6155 (Linking)
VI  - 35
IP  - 1
DP  - 2020 Mar
TI  - Regulating of cell cycle progression by the lncRNA CDKN2B-AS1/miR-324-5p/ROCK1 
      axis in laryngeal squamous cell cancer.
PG  - 47-56
LID - 10.1177/1724600819898489 [doi]
AB  - OBJECTIVE: To evaluate the function of long non-coding RNA ANRIL (CDKN2B-AS1) in 
      laryngeal squamous cell cancer (LSCC), and to explore the underlying mechanism. 
      METHODS: The expression levels of CDKN2B-AS1 in LSCC tissues and cell lines 
      (Tu177, HN4, AMC-HN-8 and NP69) were determined by reverse transcription 
      quantitative PCR (RT-qPCR). AMC-HN-8 cells were then transfected with siRNAs of 
      CDKN2B-AS1. The effects of CDKN2B-AS1 on cell proliferation, cell cycle, and 
      apoptotic protein were determined by CCK-8 assay, flow cytometry analysis, and 
      western blot, respectively. Dual luciferase reporter assay and RNA 
      immunoprecipitation (RIP) assay were employed to verify the targets of 
      CDKN2B-AS1. The miR-324-5p mimics or miR-324-5p inhibitor and ROCK1 
      over-expression plasmids were also transfected into AMC-HN-8 cells for further 
      analysis. RESULTS: CDKN2B-AS1 was upregulated in LSCC tissues, and the 
      upregulation of CDKN2B-AS1 was correlated with overall survival, advanced 
      clinical stage, and lymph node metastasis. In AMC-HN-8 cells, the knockdown of 
      CDKN2B-AS1 by siRNA inhibited cell viability, blocked cell cycle in G1 phase, and 
      increased the expression levels of cyclin-dependent kinase inhibitor 1A (p21), 
      cleaved caspase3, and cleaved PPoly (ADP-Ribose) polymerase 1. Results of dual 
      luciferase reporter assay showed that miR-324-5p could bind to CDKN2B-AS1 or 
      Rho-associated coiled-coil containing protein kinase 1 (ROCK1). Finally, 
      over-expression of ROCK1 in AMC-HN-8 cells revised the inhibitory effect of 
      CDKN2B-AS1 siRNA on cell growth. DISCUSSION: The upregulation of CDKN2B-AS1 was 
      correlated with overall survival, advanced clinical stage, and lymph node 
      metastasis and promoted LSCC cell growth via miR-324-5p/ROCK1 axis.
FAU - Liu, Feifei
AU  - Liu F
AD  - Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, 
      Capital Medical University, Beijing, China.
AD  - Department of Otolaryngology-Head and Neck Surgery, Affiliated Yantai Yuhuangding 
      Hospital of Qingdao University, Yantai, Shandong Province, China.
FAU - Xiao, Yang
AU  - Xiao Y
AD  - Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, 
      Capital Medical University, Beijing, China.
FAU - Ma, Lijing
AU  - Ma L
AD  - Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, 
      Capital Medical University, Beijing, China.
FAU - Wang, Jun
AU  - Wang J
AUID- ORCID: 0000-0002-6387-5926
AD  - Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, 
      Capital Medical University, Beijing, China.
LA  - eng
PT  - Journal Article
DEP - 20200121
PL  - United States
TA  - Int J Biol Markers
JT  - The International journal of biological markers
JID - 8712411
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (CDKN2B antisense RNA, human)
RN  - 0 (MIRN324 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
RN  - EC 2.7.11.1 (ROCK1 protein, human)
RN  - EC 2.7.11.1 (rho-Associated Kinases)
SB  - IM
MH  - Base Sequence
MH  - Biomarkers, Tumor/genetics/metabolism
MH  - Cell Cycle/physiology
MH  - Cell Growth Processes/physiology
MH  - Cell Line, Tumor
MH  - Gene Knockdown Techniques
MH  - HEK293 Cells
MH  - Humans
MH  - Laryngeal Neoplasms/genetics/*metabolism/pathology
MH  - MicroRNAs/biosynthesis/genetics/*metabolism
MH  - RNA, Long Noncoding/biosynthesis/genetics/*metabolism
MH  - Signal Transduction
MH  - Squamous Cell Carcinoma of Head and Neck/genetics/*metabolism/pathology
MH  - Up-Regulation
MH  - rho-Associated Kinases/genetics/*metabolism
OTO - NOTNLM
OT  - CDKN2B-AS1
OT  - ROCK1
OT  - cell growth
OT  - laryngeal squamous cell cancer
OT  - miR-324-5P
EDAT- 2020/01/22 06:00
MHDA- 2020/12/02 06:00
CRDT- 2020/01/22 06:00
PHST- 2020/01/22 06:00 [pubmed]
PHST- 2020/12/02 06:00 [medline]
PHST- 2020/01/22 06:00 [entrez]
AID - 10.1177/1724600819898489 [doi]
PST - ppublish
SO  - Int J Biol Markers. 2020 Mar;35(1):47-56. doi: 10.1177/1724600819898489. Epub 
      2020 Jan 21.