PMID- 31960838
OWN - NLM
STAT- MEDLINE
DCOM- 20201209
LR  - 20201214
IS  - 1364-5528 (Electronic)
IS  - 0003-2654 (Linking)
VI  - 145
IP  - 6
DP  - 2020 Mar 21
TI  - Biosensing extracellular vesicles: contribution of biomolecules in affinity-based 
      methods for detection and isolation.
PG  - 1997-2013
LID - 10.1039/c9an01949a [doi]
AB  - Extracellular Vesicles (EVs) are lipid vesicles secreted by cells that allow 
      intercellular communication. They are decorated with surface proteins, which are 
      membrane proteins that can be targeted by biochemical techniques to isolate EVs 
      from background particles. EVs have recently attracted attention for their 
      potential applications as biomarkers for numerous diseases. This review focuses 
      on the contribution of biomolecules used as ligands in affinity-based biosensors 
      for the detection and isolation of EVs. Capturing biological objects like EVs 
      with antibodies is well described in the literature through different biosensing 
      techniques. However, since handling proteins can be challenging due to stability 
      issues, sensors using non-denaturable biomolecules are emerging. DNA aptamers, 
      short DNA fragments that mimic antibody action, are currently being developed and 
      considered as the future of antibody-like ligands. These molecules offer 
      undeniable advantages: unparalleled ease of production, very high stability in 
      air, similar affinity constants to antibodies, and compatibility with many 
      organic solvents. The use of peptides specific to EVs is also an exciting 
      biochemical solution to target EV membrane proteins and complement other probes. 
      These different ligands have been used in several types of biosensors: 
      electrochemical, optical, microfluidic using both generic probes (targeting 
      widely expressed membrane proteins such as the tetraspanins) and specific probes 
      (targeting disease biomarkers such as proteins overexpressed in cancer).
FAU - Gaillard, M
AU  - Gaillard M
AD  - Univ. Grenoble Alpes, CEA, CNRS, IRIG, SyMMES, 38000 Grenoble, France. 
      camille.raillon@cea.fr yoann.roupioz@cea.fr.
FAU - Thuaire, A
AU  - Thuaire A
FAU - Nonglaton, G
AU  - Nonglaton G
FAU - Agache, V
AU  - Agache V
FAU - Roupioz, Y
AU  - Roupioz Y
FAU - Raillon, C
AU  - Raillon C
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20200121
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - 0 (Antibodies, Immobilized)
RN  - 0 (Aptamers, Nucleotide)
RN  - 0 (Biomarkers)
SB  - IM
MH  - Animals
MH  - Antibodies, Immobilized/chemistry
MH  - Aptamers, Nucleotide/chemistry
MH  - Biomarkers/analysis
MH  - Biosensing Techniques/instrumentation/*methods
MH  - Electrochemical Techniques/instrumentation/methods
MH  - Equipment Design
MH  - Extracellular Vesicles/*chemistry/pathology
MH  - Flow Cytometry/instrumentation/methods
MH  - Humans
MH  - Microfluidic Analytical Techniques/instrumentation/methods
MH  - Neoplasms/diagnosis
EDAT- 2020/01/22 06:00
MHDA- 2020/12/15 06:00
CRDT- 2020/01/22 06:00
PHST- 2020/01/22 06:00 [pubmed]
PHST- 2020/12/15 06:00 [medline]
PHST- 2020/01/22 06:00 [entrez]
AID - 10.1039/c9an01949a [doi]
PST - ppublish
SO  - Analyst. 2020 Mar 21;145(6):1997-2013. doi: 10.1039/c9an01949a. Epub 2020 Jan 21.