PMID- 31961136
OWN - NLM
STAT- MEDLINE
DCOM- 20210101
LR  - 20221207
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 92
IP  - 4
DP  - 2020 Feb 18
TI  - Application of a Pseudotargeted MS Method for the Quantification of Glycated 
      Hemoglobin for the Improved Diagnosis of Diabetes Mellitus.
PG  - 3237-3245
LID - 10.1021/acs.analchem.9b05046 [doi]
AB  - Proteins in a human body continuously undergo glycation reactions with reducing 
      sugars, forming early as well as advanced glycation end-products (AGEs) which are 
      highly disease-relevant. Specifically, N-1-(deoxyfructosyl) valine of 
      beta-hemoglobin (HbA1c) has been considered as a marker of diabetes, but the exact 
      map of glycated Hb peptides corelated with diabetes in different stages is poorly 
      studied. Here, the pseudotargeted parallel reaction monitoring (PRM) method 
      combined with relative retention time (iRT) endogenous peptides was proposed for 
      exploring the roles of deoxyfructosyl (DF-), carboxymethyl (CM-), and 
      carboxyethyl (CE-) based Hb modifications in clinical prognosis and diagnosis of 
      type 2 diabetes mellitus (T2DM) and its complication. For building the 
      pseudotargeted list, data-dependent acquisition (DDA) combined with multiple 
      enzyme digestion was employed for the comprehensive identification of the three 
      types of modification in vitro Hb and in vivo Hb. The introduction of the 
      endogenous iRT peptides during PRM analysis facilitates being able to obtain 
      accurate quantitative results. When applying this new strategy to quantify the 
      three kinds of glycated Hb peptides in clinical samples, patients with T2DM in 
      different pathophysiological conditions were fully distinguished from the 
      controls, indicating the necessity of adopting multiple glycation types for the 
      improved diagnosis of T2DM. Taken together, the newly developed pseudotargeted 
      PRM method not only expands the horizons of glycated Hb by reliably assessing the 
      actual status of T2DM but also reveals that endogenous iRT might be a viable 
      option for label-free quantitative analysis.
FAU - Li, Weifeng
AU  - Li W
AUID- ORCID: 0000-0002-5876-0606
AD  - Shenzhen Second People's Hospital , The First Affiliated Hospital of Shenzhen 
      University , Shenzhen 518020 , China.
AD  - Integrated Chinese and Western Medicine Postdoctoral Research Station , Jinan 
      University , Guangzhou 510632 , China.
FAU - Lin, Lin
AU  - Lin L
AUID- ORCID: 0000-0002-4529-6779
AD  - Sustech Core Research Facilities , Southern University of Science and Technology 
      , Shenzhen 518055 , China.
FAU - Yan, Dewen
AU  - Yan D
AD  - Shenzhen Second People's Hospital , The First Affiliated Hospital of Shenzhen 
      University , Shenzhen 518020 , China.
FAU - Jin, Yu
AU  - Jin Y
AD  - Shenzhen Second People's Hospital , The First Affiliated Hospital of Shenzhen 
      University , Shenzhen 518020 , China.
FAU - Xu, Yun
AU  - Xu Y
AD  - Shenzhen Second People's Hospital , The First Affiliated Hospital of Shenzhen 
      University , Shenzhen 518020 , China.
FAU - Li, Yinghong
AU  - Li Y
AD  - Shenzhen Second People's Hospital , The First Affiliated Hospital of Shenzhen 
      University , Shenzhen 518020 , China.
FAU - Ma, Min
AU  - Ma M
AD  - Integrated Chinese and Western Medicine Postdoctoral Research Station , Jinan 
      University , Guangzhou 510632 , China.
FAU - Wu, Zhengzhi
AU  - Wu Z
AUID- ORCID: 0000-0002-8819-609X
AD  - Shenzhen Second People's Hospital , The First Affiliated Hospital of Shenzhen 
      University , Shenzhen 518020 , China.
AD  - Integrated Chinese and Western Medicine Postdoctoral Research Station , Jinan 
      University , Guangzhou 510632 , China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200131
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Glycated Hemoglobin A)
SB  - IM
MH  - Amino Acid Sequence
MH  - Diabetes Mellitus, Type 2/*blood/*diagnosis
MH  - Glycated Hemoglobin/*analysis/chemistry
MH  - Humans
MH  - Mass Spectrometry/*methods
MH  - Models, Molecular
MH  - Protein Conformation
EDAT- 2020/01/22 06:00
MHDA- 2021/01/02 06:00
CRDT- 2020/01/22 06:00
PHST- 2020/01/22 06:00 [pubmed]
PHST- 2021/01/02 06:00 [medline]
PHST- 2020/01/22 06:00 [entrez]
AID - 10.1021/acs.analchem.9b05046 [doi]
PST - ppublish
SO  - Anal Chem. 2020 Feb 18;92(4):3237-3245. doi: 10.1021/acs.analchem.9b05046. Epub 
      2020 Jan 31.