PMID- 31962207
OWN - NLM
STAT- MEDLINE
DCOM- 20200420
LR  - 20200420
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 1139
DP  - 2020 Feb 15
TI  - Hollow-fibre liquid-phase microextraction and gas chromatography-mass 
      spectrometric determination of amphetamines in whole blood.
PG  - 121973
LID - S1570-0232(19)31300-5 [pii]
LID - 10.1016/j.jchromb.2020.121973 [doi]
AB  - Here, we present a fully validated method using a hollow-fibre liquid-phase 
      microextraction technique for the determination by gas chromatography-mass 
      spectrometry (GC-MS) of amphetamine (AMP), methamphetamine (MET), fenproporex 
      (FEN), 3,4-methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxyamphetamine 
      (MDA) and 3,4-methylenedioxyethylamphetamine (MDEA) in whole blood. The 
      validation parameters presented successful values within those recommended by the 
      Scientific Working Group for Forensic Toxicology (SWGTox) in the Standard 
      Practices for Method Validation in Forensic Toxicology. The limits of detection 
      ranged from 1 to 3 ng/mL, and the limits of quantification ranged from 2 to 
      5 ng/mL. The determination coefficients (r(2)) ranged from 0.990 to 0.997, and 
      the method presented good intraday and interday accuracy (from 90.4% to 97.2%) 
      and satisfactory recovery (from 68% to 110%). No carryover was observed. The 
      heteroscedasticity was tested, and only AMP presented homoscedasticity. Weighting 
      factors were applied to correct the linearity of MET (1/x(2)), MDA (1/x), FEN 
      (1/x(1/2)), MDMA (1/x(2)) and MDEA (1/y). Dilution integrity was tested at ratios 
      of 1:2, 1:5 and 1:10, and all maintained intraday precision (from 94.9% to 99.3%) 
      and interday precision (from 89.4% to 94.9%). The validated method was applied to 
      six real whole blood samples from individuals suspected of consuming ecstasy, and 
      MDMA, MDA and amphetamine were successfully identified and quantified.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Bombana, Henrique Silva
AU  - Bombana HS
AD  - Faculty of Medicine, University of Sao Paulo, Av. Dr. Arnaldo, 455 Cerqueira 
      Cesar, Sao Paulo 01246-903, Brazil. Electronic address: hbombana@usp.br.
FAU - Dos Santos, Marcelo Filonzi
AU  - Dos Santos MF
AD  - Faculty of Medicine, University of Sao Paulo, Av. Dr. Arnaldo, 455 Cerqueira 
      Cesar, Sao Paulo 01246-903, Brazil.
FAU - Munoz, Daniel Romero
AU  - Munoz DR
AD  - Faculty of Medicine, University of Sao Paulo, Av. Dr. Arnaldo, 455 Cerqueira 
      Cesar, Sao Paulo 01246-903, Brazil.
FAU - Leyton, Vilma
AU  - Leyton V
AD  - Faculty of Medicine, University of Sao Paulo, Av. Dr. Arnaldo, 455 Cerqueira 
      Cesar, Sao Paulo 01246-903, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20200113
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Amphetamines)
SB  - IM
MH  - Amphetamines/*blood/chemistry/isolation & purification
MH  - Forensic Toxicology
MH  - Gas Chromatography-Mass Spectrometry/*methods
MH  - Green Chemistry Technology
MH  - Humans
MH  - Limit of Detection
MH  - Linear Models
MH  - Liquid Phase Microextraction/*methods
MH  - Reproducibility of Results
OTO - NOTNLM
OT  - Amphetamines
OT  - Green chemistry
OT  - Liquid-phase microextraction
COIS- Declaration of Competing Interest The authors have no conflicts of interest to 
      declare.
EDAT- 2020/01/22 06:00
MHDA- 2020/04/21 06:00
CRDT- 2020/01/22 06:00
PHST- 2019/08/30 00:00 [received]
PHST- 2020/01/08 00:00 [revised]
PHST- 2020/01/09 00:00 [accepted]
PHST- 2020/01/22 06:00 [pubmed]
PHST- 2020/04/21 06:00 [medline]
PHST- 2020/01/22 06:00 [entrez]
AID - S1570-0232(19)31300-5 [pii]
AID - 10.1016/j.jchromb.2020.121973 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2020 Feb 15;1139:121973. doi: 
      10.1016/j.jchromb.2020.121973. Epub 2020 Jan 13.