PMID- 31965296
OWN - NLM
STAT- MEDLINE
DCOM- 20210210
LR  - 20210210
IS  - 1432-1831 (Electronic)
IS  - 0300-8584 (Print)
IS  - 0300-8584 (Linking)
VI  - 209
IP  - 3
DP  - 2020 Jun
TI  - FISH and chips: a review of microfluidic platforms for FISH analysis.
PG  - 373-391
LID - 10.1007/s00430-019-00654-1 [doi]
AB  - Fluorescence in situ hybridization (FISH) allows visualization of specific 
      nucleic acid sequences within an intact cell or a tissue section. It is based on 
      molecular recognition between a fluorescently labeled probe that penetrates the 
      cell membrane of a fixed but intact sample and hybridizes to a nucleic acid 
      sequence of interest within the cell, rendering a measurable signal. FISH has 
      been applied to, for example, gene mapping, diagnosis of chromosomal aberrations 
      and identification of pathogens in complex samples as well as detailed studies of 
      cellular structure and function. However, FISH protocols are complex, they 
      comprise of many fixation, incubation and washing steps involving a range of 
      solvents and temperatures and are, thus, generally time consuming and labor 
      intensive. The complexity of the process, the relatively high-priced fluorescent 
      probes and the fairly high-end microscopy needed for readout render the whole 
      process costly and have limited wider uptake of this powerful technique. In 
      recent years, there have been attempts to transfer FISH assay protocols onto 
      microfluidic lab-on-a-chip platforms, which reduces the required amount of sample 
      and reagents, shortens incubation times and, thus, time to complete the protocol, 
      and finally has the potential for automating the process. Here, we review the 
      wide variety of approaches for lab-on-chip-based FISH that have been demonstrated 
      at proof-of-concept stage, ranging from FISH analysis of immobilized cell layers, 
      and cells trapped in arrays, to FISH on tissue slices. Some researchers have 
      aimed to develop simple devices that interface with existing equipment and 
      workflows, whilst others have aimed to integrate the entire FISH protocol into a 
      fully autonomous FISH on-chip system. Whilst the technical possibilities for FISH 
      on-chip are clearly demonstrated, only a small number of approaches have so far 
      been converted into off-the-shelf products for wider use beyond the research 
      laboratory.
FAU - Rodriguez-Mateos, Pablo
AU  - Rodriguez-Mateos P
AUID- ORCID: 0000-0002-7102-5024
AD  - Department of Chemistry and Biochemistry, University of Hull, Cottingham Road, 
      Hull, HU6 7RX, UK.
FAU - Azevedo, Nuno Filipe
AU  - Azevedo NF
AD  - LEPABE-Laboratory for Process Engineering, Environment, Biotechnology and Energy, 
      Department of Chemical Engineering, Faculty of Engineering of University of 
      Porto, Rua Dr. Roberto Frias, s/n, 4200-465, Porto, Portugal.
AD  - Biomode SA, Av. Mestre Jose Veiga, 4715-330, Braga, Portugal.
FAU - Almeida, Carina
AU  - Almeida C
AD  - Biomode SA, Av. Mestre Jose Veiga, 4715-330, Braga, Portugal.
AD  - INIAV, I.P.-National Institute for Agricultural and Veterinary Research, Rua dos 
      Lagidos, Lugar da Madalena, Vairao, 4485-655, Vila Do Conde, Portugal.
AD  - CEB-Centre of Biological Engineering, University of Minho, 4710-057, Braga, 
      Portugal.
FAU - Pamme, Nicole
AU  - Pamme N
AUID- ORCID: 0000-0002-9391-9387
AD  - Department of Chemistry and Biochemistry, University of Hull, Cottingham Road, 
      Hull, HU6 7RX, UK. n.pamme@hull.ac.uk.
LA  - eng
GR  - 765042/H2020 Marie Sklodowska-Curie Actions/
PT  - Journal Article
PT  - Review
DEP - 20200121
PL  - Germany
TA  - Med Microbiol Immunol
JT  - Medical microbiology and immunology
JID - 0314524
SB  - IM
MH  - Clinical Laboratory Techniques/instrumentation/methods
MH  - In Situ Hybridization, Fluorescence/*instrumentation/*methods
MH  - *Lab-On-A-Chip Devices
MH  - Microfluidic Analytical Techniques/*instrumentation/*methods
PMC - PMC7248050
OTO - NOTNLM
OT  - Fluorescence in situ hybridization (FISH)
OT  - Lab-on-a-Chip (LOC)
OT  - Microfluidics
OT  - Microfluidics-assisted FISH
OT  - microFISH
EDAT- 2020/01/23 06:00
MHDA- 2021/02/11 06:00
PMCR- 2020/01/21
CRDT- 2020/01/23 06:00
PHST- 2019/08/29 00:00 [received]
PHST- 2019/12/19 00:00 [accepted]
PHST- 2020/01/23 06:00 [pubmed]
PHST- 2021/02/11 06:00 [medline]
PHST- 2020/01/23 06:00 [entrez]
PHST- 2020/01/21 00:00 [pmc-release]
AID - 10.1007/s00430-019-00654-1 [pii]
AID - 654 [pii]
AID - 10.1007/s00430-019-00654-1 [doi]
PST - ppublish
SO  - Med Microbiol Immunol. 2020 Jun;209(3):373-391. doi: 10.1007/s00430-019-00654-1. 
      Epub 2020 Jan 21.