PMID- 31966224
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231011
IS  - 1810-522X (Electronic)
IS  - 1021-5506 (Print)
IS  - 1021-5506 (Linking)
VI  - 56
DP  - 2017
TI  - Cytogenetics of Two Onychostoma Species in Taiwan by Ag-NOR and 18S rDNA 
      Profiles.
PG  - e25
LID - 10.6620/ZS.2017.56-25 [doi]
LID - e25
AB  - Chiao-Chuan Han, Tsair-Bor Yen, Nian-Cih Chen, and Mei-Chen Tseng (2017) Both 
      Onychostoma barbatulum and O. alticorpus are primary freshwater fish in Taiwan. 
      The former has been developed as an aquaculture species with high economic value, 
      while the latter is a native endemic species in Taiwan. Understanding the 
      cytogenetic information of these two species is necessary for their selected 
      breeding, recovery, and management. In this study, Giemsa staining, 
      silver-binding nucleolar organizer region (Ag-NOR), C-banding, and fluorescence 
      in situ hybridization (FISH) with 18S ribosomal (r)DNA probes were used to 
      analyze the cytogenetic characteristics. Results of Giemsa staining showed that 
      the two Onychostoma species shared the same number of chromosomes, 2n = 50. 
      Respective karyotype formulas of the female and male were 10 m + 22 sm + 10 st + 
      8 t and 11 m + 22 sm + 10 st + 7 t in O. barbatulum, and 14 m + 18 sm + 8 st + 10 
      t and 15 m + 18 sm + 8 st + 9 t in O. alticorpus. Karyotypes of both species 
      showed a pair of heteromorphic chromosomes in male fish. Their sex determination 
      should be the XX/XY system. Two pairs of Ag-NORs were found in O. barbatulum, but 
      only one pair occurred in O. alticorpus. C-banding areas were observed on 
      centromeres or telomeres of some chromosomes. FISH revealed different cytogenetic 
      characters between these two species. The above cytogenetic information will 
      contribute to species identification, population recovery, and advantages for 
      breeding and management in the future.
FAU - Han, Chiao-Chuan
AU  - Han CC
AD  - National Museum of Marine Biology and Aquarium, Pingtung 944, Taiwan. E-mail: 
      hancc@nmmba.gov.tw.
AD  - Graduate Institute of Marine Biology, National Dong Hwa University, Hualien 974, 
      Taiwan.
FAU - Yen, Tsair-Bor
AU  - Yen TB
AD  - Department of Tropical Agriculture and International Cooperation, National 
      Pingtung University of Science and Technology, Pingtung 912, Taiwan. E-mail: 
      tbyen@mail.npust.edu.tw.
FAU - Chen, Nian-Cih
AU  - Chen NC
AD  - Department of Aquaculture, National Pingtung University of Science and 
      Technology, Pingtung 912, Taiwan.
FAU - Tseng, Mei-Chen
AU  - Tseng MC
AD  - Department of Aquaculture, National Pingtung University of Science and 
      Technology, Pingtung 912, Taiwan.
LA  - eng
PT  - Journal Article
DEP - 20170925
PL  - China (Republic : 1949- )
TA  - Zool Stud
JT  - Zoological studies
JID - 9889708
PMC - PMC6517726
OTO - NOTNLM
OT  - Endemic species
OT  - Fluorescence in situ hybridization
OT  - Karyotype
OT  - Sex determination
OT  - Species identification
EDAT- 2017/09/25 00:00
MHDA- 2017/09/25 00:01
PMCR- 2017/09/25
CRDT- 2020/01/23 06:00
PHST- 2017/06/05 00:00 [received]
PHST- 2017/09/05 00:00 [accepted]
PHST- 2020/01/23 06:00 [entrez]
PHST- 2017/09/25 00:00 [pubmed]
PHST- 2017/09/25 00:01 [medline]
PHST- 2017/09/25 00:00 [pmc-release]
AID - 10.6620/ZS.2017.56-25 [doi]
PST - epublish
SO  - Zool Stud. 2017 Sep 25;56:e25. doi: 10.6620/ZS.2017.56-25. eCollection 2017.