PMID- 31966351
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200126
IS  - 1936-2625 (Electronic)
IS  - 1936-2625 (Linking)
VI  - 10
IP  - 10
DP  - 2017
TI  - Recognition of the human antibody-mediated platelet destruction in adult ITP 
      patients by C-reactive protein.
PG  - 10176-10185
AB  - Immune thrombocytopenia purpura (ITP) is characterized by destruction of 
      circulating platelets and the presence of antiplatelet IgG antibodies, which 
      opsonize platelets for splenic clearance resulting in low levels of circulating 
      platelets, and the disease severity can be predicted neither by antibody isotype 
      nor by titer, indicating that other factors also play a role. Although the main 
      cause of ITP remains unclear, but its relationship with some infection was 
      demonstrated, including viral or bacterial infections. C-reactive protein (CRP), 
      a member of the pentraxin family, is a major acute-phase protein in humans and is 
      a clinical marker of infection. We aimed to investigate the correlation between 
      the levels of CRP and the presence of antiplatelet IgG antibodies in adults with 
      newly diagnosed ITP. CRP levels and platelet counts were measured in the blood 
      samples from a 60 ITP patient (with confirmed anti-GPIIb/IIIa antibodies), 60 
      infection patients (all without anti-GPIIb/IIIa antibodies) and 60 normal 
      individuals. The bleeding score, recover time of intravenous immune globulin 
      (IVIg) therapy and the number of megakaryocytes in bone marrow were recorded in 
      ITP patients. The platelet count, bleeding score, recover time of intravenous 
      immune globulin (IVIG) therapy and the number of megakaryocytes in bone marrow 
      and CRP concentrations were compared in ITP group using Spearman's correlation 
      coefficient. We examined the influence of intraperioneal CRP administration on 
      antibody-mediated platelet destruction in mice. There were no statistical 
      differences in gender, age and body mass index among the three groups (P>0.05). 
      Though CRP levels are significantly elevated in ITP patients and infection 
      patients (P<0.05), the platelet count was markedly lower only in ITP patients. We 
      found that CRP was inert toward platelets without antiplatelet antibodies in this 
      study. There are a significant correlation between CRP levels and platelet 
      counts, bleeding severity and the number of megakaryocytes in bone marrow 
      aspiration (r=-0.5079, r=0.5498, r=0.4172, P<0.001, respectively). Moreover, a 
      significant correlation was observed between the recovery time of platelet count 
      and CRP levels (r=-0.5569, P<0.001). In mice, platelet count was lower in 
      Anti-CD41 (0.75 mug)+, CRP (200 mug) group as compared with Anti-CD41 (0.75 mug)+, 
      CRP(-) group and Anti-CD41 (0.75 mug)-, CRP (200 mug) group (P<0.05). In summary, 
      this study indicated that CRP levels are significantly elevated in ITP patients 
      all with confirmed anti-GPIIb/IIIa antibodies, which is able to predict the 
      clinical bleeding severity of ITP patients. The slower CRP levels reduction after 
      IVIg treatment predicted slower platelet count recovery in ITP.
CI  - IJCEP Copyright (c) 2017.
FAU - Pan, Jia-Qi
AU  - Pan JQ
AD  - Department of Hematology, The Second Affiliated Hospital of Harbin Medical 
      University Nangang, Harbin, Heilongjiang, P. R. China.
FAU - Wang, Wei
AU  - Wang W
AD  - Department of Hematology, The Second Affiliated Hospital of Harbin Medical 
      University Nangang, Harbin, Heilongjiang, P. R. China.
FAU - Wang, Meng-Lin
AU  - Wang ML
AD  - Department of Hematology, The Second Affiliated Hospital of Harbin Medical 
      University Nangang, Harbin, Heilongjiang, P. R. China.
FAU - Li, Xiao-Yun
AU  - Li XY
AD  - Department of Hematology, The Second Affiliated Hospital of Harbin Medical 
      University Nangang, Harbin, Heilongjiang, P. R. China.
FAU - Wang, Jing-Hua
AU  - Wang JH
AD  - Department of Hematology, The Second Affiliated Hospital of Harbin Medical 
      University Nangang, Harbin, Heilongjiang, P. R. China.
FAU - Zhao, Wei-Wei
AU  - Zhao WW
AD  - Department of Hematology, The Second Affiliated Hospital of Harbin Medical 
      University Nangang, Harbin, Heilongjiang, P. R. China.
FAU - Tian, Yao-Yao
AU  - Tian YY
AD  - Department of Hematology, The Second Affiliated Hospital of Harbin Medical 
      University Nangang, Harbin, Heilongjiang, P. R. China.
FAU - Dong, Xiu-Shuai
AU  - Dong XS
AD  - Department of Hematology, The Second Affiliated Hospital of Harbin Medical 
      University Nangang, Harbin, Heilongjiang, P. R. China.
FAU - Jiang, Yong-Fang
AU  - Jiang YF
AD  - Department of Hematology, The Second Affiliated Hospital of Harbin Medical 
      University Nangang, Harbin, Heilongjiang, P. R. China.
LA  - eng
PT  - Journal Article
DEP - 20171001
PL  - United States
TA  - Int J Clin Exp Pathol
JT  - International journal of clinical and experimental pathology
JID - 101480565
PMC - PMC6965790
OTO - NOTNLM
OT  - C-reactive protein
OT  - antiplatelet antibodies
OT  - immune thrombocytopenic purpura
COIS- None.
EDAT- 2017/10/01 00:00
MHDA- 2017/10/01 00:01
PMCR- 2017/10/01
CRDT- 2020/01/23 06:00
PHST- 2017/05/26 00:00 [received]
PHST- 2017/08/02 00:00 [accepted]
PHST- 2020/01/23 06:00 [entrez]
PHST- 2017/10/01 00:00 [pubmed]
PHST- 2017/10/01 00:01 [medline]
PHST- 2017/10/01 00:00 [pmc-release]
PST - epublish
SO  - Int J Clin Exp Pathol. 2017 Oct 1;10(10):10176-10185. eCollection 2017.