PMID- 31966417
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200126
IS  - 1936-2625 (Electronic)
IS  - 1936-2625 (Linking)
VI  - 10
IP  - 11
DP  - 2017
TI  - Bcl-xL expression improves the therapeutic effect of human umbilical cord stem 
      cell transplantation on articular cartilage injury in rabbit.
PG  - 10740-10750
AB  - BACKGROUND: To investigate the therapeutic effect of transplantation of B-cell 
      lymphoma-extra large (Bcl-xL) gene modified human umbilical cord blood stem cells 
      (HUCSCs) on rabbit articular cartilage injury. MATERIALS AND METHODS: HUCSCs were 
      isolated and identified. Lentiviral encoding Bcl-xL was applied to modify HUCSCs. 
      The effects of Bcl-xL overexpression on apoptosis and related gene expression 
      after differentiation induction of HUCSCs were detected. Additionally, the 
      efficiency of transplantation of Bcl-xL gene modified HUCSCs on articular 
      cartilage injury were evaluated. RESULTS: HUCSCs could differentiate into 
      chondrocytes after induction. Compared with control group, the apoptosis after 
      induction was significantly elevated, but reduced by Bcl-xL gene overexpression. 
      The differentiation of HUCSCs into chondrocytes was displayed by expression of 
      type II collagen (CII), but accompanying with expression of caspase-3 and matrix 
      metalloproteinase-3 (MMP-3). By contrast, Bcl-xL gene overexpression reduced 
      caspase-3 and MMP-3 expression, but further increased CII expression. 
      Pathological staining showed that Bcl-xL gene modified HUCSCs could obviously 
      repair cartilage injury. Compared with sham control group, the expression of 
      caspase-3 and MMP-3 in model group was significantly up-regulated, while the 
      expression of CII was significantly down-regulated. Transplantation of HUCSCs 
      could ameliorate the injury, while Bcl-xL modification could improve the 
      therapeutic effect of transplantation of HUCSCs. Moreover, Bcl-xL modification 
      could further decrease cartilage injury-induced expression of caspase-3 and 
      MMP-3, and improve the expression of CII compared with transplantation of normal 
      HUCSCs. CONCLUSIONS: Bcl-xL gene modification decreases cell 
      differentiation-induced apoptosis and improves the efficiency of HUCSCs 
      transplantation in the repairing of cartilage injury.
CI  - IJCEP Copyright (c) 2017.
FAU - Pan, Zhengjun
AU  - Pan Z
AD  - Department of Joint Surgery, The Third Affiliated Hospital of Anhui Medical 
      University Hefei, China.
FAU - Yin, Hao
AU  - Yin H
AD  - Department of Joint Surgery, The Third Affiliated Hospital of Anhui Medical 
      University Hefei, China.
FAU - Wang, Shuangli
AU  - Wang S
AD  - Department of Joint Surgery, The Third Affiliated Hospital of Anhui Medical 
      University Hefei, China.
FAU - Xiong, Gaoxin
AU  - Xiong G
AD  - Department of Joint Surgery, The Third Affiliated Hospital of Anhui Medical 
      University Hefei, China.
FAU - Yin, Zongsheng
AU  - Yin Z
AD  - Department of Joint Surgery, The First Affiliated Hospital of Anhui Medical 
      University Hefei, China.
LA  - eng
PT  - Journal Article
DEP - 20171101
PL  - United States
TA  - Int J Clin Exp Pathol
JT  - International journal of clinical and experimental pathology
JID - 101480565
PMC - PMC6965849
OTO - NOTNLM
OT  - Bcl-xL
OT  - articular cartilage injury
OT  - human umbilical cord stem cells
OT  - transplantation
COIS- None.
EDAT- 2017/11/01 00:00
MHDA- 2017/11/01 00:01
PMCR- 2017/11/01
CRDT- 2020/01/23 06:00
PHST- 2017/09/03 00:00 [received]
PHST- 2017/10/18 00:00 [accepted]
PHST- 2020/01/23 06:00 [entrez]
PHST- 2017/11/01 00:00 [pubmed]
PHST- 2017/11/01 00:01 [medline]
PHST- 2017/11/01 00:00 [pmc-release]
PST - epublish
SO  - Int J Clin Exp Pathol. 2017 Nov 1;10(11):10740-10750. eCollection 2017.