PMID- 31966812
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200126
IS  - 1936-2625 (Electronic)
IS  - 1936-2625 (Linking)
VI  - 10
IP  - 9
DP  - 2017
TI  - UHMWPE wear particles and dendritic cells promote osteoclastogenesis of RAW264.7 
      cells through RANK-activated NF-kappaB/MAPK/AKT pathways.
PG  - 9400-9408
AB  - Dendritic cells (DCs) were shown to enhance UHMWPE particle promoted 
      osteoclastogenesis in RAW264.7 macrophages. This study aimed to elucidate the 
      signaling network mediating the osteoclastogenic effects of UHMWPE particles and 
      DCs. RAW264.7 cells were induced with UHMWPE particles and /or DC2.4 cells. The 
      expression of Rank was silenced by shRNA. The activation of p38MAPK, AKT, and 
      NF-kappaB was inhibited specific inhibitors. The osteoclasts were assessed by TRAP 
      staining. The expression of osteoclastogenic genes and activation of p38MAPK, 
      AKT, and NF-kappaB were analyzed by qPCR and/or Western blot. UHMWPE particles and 
      DC2.4 cells cooperatively induced RAW264.7 macrophages to differentiate into 
      osteoclasts, activated p38MAPK, AKT, and NF-kappaB pathways, and upregulated Nfatc1 
      and osteoclast markers, which was abolished by silencing Rank or inhibited by 
      inhibitors of p38MAPK, AKT, and NF-kappaB in RAW264.7 cells. These data demonstrated 
      the complexity of signaling network and cell-cell interactions involved in UHMWPE 
      particles-induced osteolysis.
CI  - IJCEP Copyright (c) 2017.
FAU - Zhang, Jianzhi
AU  - Zhang J
AD  - Central Laboratory, Affiliated Hospital of Xuzhou Medical University Xuzhou, 
      Jiangsu, China.
FAU - Zheng, Xin
AU  - Zheng X
AD  - Department of Orthopedics, Affiliated Hospital of Xuzhou Medical University 
      Xuzhou, Jiangsu, China.
FAU - Zhao, Fengchao
AU  - Zhao F
AD  - Department of Orthopedics, Affiliated Hospital of Xuzhou Medical University 
      Xuzhou, Jiangsu, China.
FAU - Liu, Xiaoyun
AU  - Liu X
AD  - Central Laboratory, Affiliated Hospital of Xuzhou Medical University Xuzhou, 
      Jiangsu, China.
FAU - Pang, Yong
AU  - Pang Y
AD  - Department of Orthopedics, Affiliated Hospital of Xuzhou Medical University 
      Xuzhou, Jiangsu, China.
FAU - Cheng, Qi
AU  - Cheng Q
AD  - Department of Orthopedics, Affiliated Hospital of Xuzhou Medical University 
      Xuzhou, Jiangsu, China.
FAU - Wang, Yi
AU  - Wang Y
AD  - Department of Orthopedics, Affiliated Hospital of Xuzhou Medical University 
      Xuzhou, Jiangsu, China.
FAU - Zhu, Yi
AU  - Zhu Y
AD  - Department of Orthopedics, Affiliated Hospital of Xuzhou Medical University 
      Xuzhou, Jiangsu, China.
FAU - Zhang, Yan
AU  - Zhang Y
AD  - Department of Orthopedics, Affiliated Hospital of Xuzhou Medical University 
      Xuzhou, Jiangsu, China.
LA  - eng
PT  - Journal Article
DEP - 20170901
PL  - United States
TA  - Int J Clin Exp Pathol
JT  - International journal of clinical and experimental pathology
JID - 101480565
PMC - PMC6965995
OTO - NOTNLM
OT  - RNAKL/RANK
OT  - UHMWPE wear particle
OT  - dendritic cells
OT  - osteoclastogenesis
COIS- None.
EDAT- 2017/09/01 00:00
MHDA- 2017/09/01 00:01
PMCR- 2017/09/01
CRDT- 2020/01/23 06:00
PHST- 2017/05/04 00:00 [received]
PHST- 2017/07/19 00:00 [accepted]
PHST- 2020/01/23 06:00 [entrez]
PHST- 2017/09/01 00:00 [pubmed]
PHST- 2017/09/01 00:01 [medline]
PHST- 2017/09/01 00:00 [pmc-release]
PST - epublish
SO  - Int J Clin Exp Pathol. 2017 Sep 1;10(9):9400-9408. eCollection 2017.