PMID- 31969878
OWN - NLM
STAT- MEDLINE
DCOM- 20201102
LR  - 20201102
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 10
DP  - 2019
TI  - Helicobacter pylori Dampens HLA-II Expression on Macrophages via the 
      Up-Regulation of miRNAs Targeting CIITA.
PG  - 2923
LID - 10.3389/fimmu.2019.02923 [doi]
LID - 2923
AB  - Macrophages have a major role in infectious and inflammatory diseases, and the 
      available data suggest that Helicobacter pylori persistence can be explained in 
      part by the failure of the bacterium to be killed by professional phagocytes. 
      Macrophages are cells ready to kill the engulfed pathogen, through 
      oxygen-dependent and -independent mechanisms; however, their killing potential 
      can be further augmented by the intervention of T helper (Th) cells upon the 
      specific recognition of human leukocyte antigen (HLA)-II-peptide complexes on the 
      surface of the phagocytic cells. As it pertains to H. pylori, the bacterium is 
      engulfed by macrophages, but it interferes with the phagosome maturation process 
      leading to phagosomes with an altered degradative capacity, and to megasomes, 
      wherein H. pylori resists killing. We recently showed that macrophages infected 
      with H. pylori strongly reduce the expression of HLA-II molecules on the plasma 
      membrane and this compromises the bacterial antigen presentation to Th 
      lymphocytes. In this work, we demonstrate that H. pylori hampers HLA-II 
      expression in macrophages, activated or non-activated by IFN-gamma, by 
      down-regulating the expression of the class II major histocompatibility complex 
      transactivator (CIITA), the "master control factor" for the expression of HLA 
      class II genes. We provided evidence that this effect relies on the up-regulation 
      of let-7f-5p, let-7i-5p, miR-146b-5p, and -185-5p targeting CIITA. MiRNA 
      expression analysis performed on biopsies from H. pylori-infected patients 
      confirmed the up-regulation of let-7i-5p, miR-146b-5p, and -185-5p in gastritis, 
      in pre-invasive lesions, and in gastric cancer. Taken together, our results 
      suggest that specific miRNAs may be directly involved in the H. pylori infection 
      persistence and may contribute to confer the risk of developing gastric neoplasia 
      in infected patients.
CI  - Copyright (c) 2020 Codolo, Toffoletto, Chemello, Coletta, Soler Teixidor, 
      Battaggia, Munari, Fassan, Cagnin and de Bernard.
FAU - Codolo, Gaia
AU  - Codolo G
AD  - Department of Biology, University of Padua, Padua, Italy.
FAU - Toffoletto, Marta
AU  - Toffoletto M
AD  - Department of Biology, University of Padua, Padua, Italy.
FAU - Chemello, Francesco
AU  - Chemello F
AD  - Department of Biology, University of Padua, Padua, Italy.
AD  - CRIBI Biotechnology Center, University of Padua, Padua, Italy.
FAU - Coletta, Sara
AU  - Coletta S
AD  - Department of Biology, University of Padua, Padua, Italy.
FAU - Soler Teixidor, Gemma
AU  - Soler Teixidor G
AD  - Department of Biology, University of Padua, Padua, Italy.
FAU - Battaggia, Greta
AU  - Battaggia G
AD  - Department of Biology, University of Padua, Padua, Italy.
FAU - Munari, Giada
AU  - Munari G
AD  - Istituto Oncologico Veneto (IRCCS), Padua, Italy.
FAU - Fassan, Matteo
AU  - Fassan M
AD  - Department of Medicine, University of Padua, Padua, Italy.
FAU - Cagnin, Stefano
AU  - Cagnin S
AD  - Department of Biology, University of Padua, Padua, Italy.
AD  - CRIBI Biotechnology Center, University of Padua, Padua, Italy.
AD  - CIR-Myo Myology Center, University of Padua, Padua, Italy.
FAU - de Bernard, Marina
AU  - de Bernard M
AD  - Department of Biology, University of Padua, Padua, Italy.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200108
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
RN  - 0 (HLA Antigens)
RN  - 0 (MHC class II transactivator protein)
RN  - 0 (MicroRNAs)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Trans-Activators)
SB  - IM
MH  - Aged
MH  - Cell Line, Tumor
MH  - Cells, Cultured
MH  - Female
MH  - Gene Expression Profiling/methods
MH  - HLA Antigens/*immunology
MH  - Helicobacter Infections/*immunology
MH  - Helicobacter pylori/*immunology
MH  - Humans
MH  - Macrophages/*immunology
MH  - Male
MH  - MicroRNAs/*immunology
MH  - Middle Aged
MH  - Nuclear Proteins/*immunology
MH  - Stomach Neoplasms/immunology
MH  - Trans-Activators/*immunology
MH  - Up-Regulation/*immunology
PMC - PMC6960189
OTO - NOTNLM
OT  - Helicobacter pylori
OT  - antigen presentation
OT  - gastric cancer
OT  - macrophages
OT  - miRNA
EDAT- 2020/01/24 06:00
MHDA- 2020/11/03 06:00
PMCR- 2019/01/01
CRDT- 2020/01/24 06:00
PHST- 2019/06/13 00:00 [received]
PHST- 2019/11/28 00:00 [accepted]
PHST- 2020/01/24 06:00 [entrez]
PHST- 2020/01/24 06:00 [pubmed]
PHST- 2020/11/03 06:00 [medline]
PHST- 2019/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2019.02923 [doi]
PST - epublish
SO  - Front Immunol. 2020 Jan 8;10:2923. doi: 10.3389/fimmu.2019.02923. eCollection 
      2019.