PMID- 31970435
OWN - NLM
STAT- MEDLINE
DCOM- 20200511
LR  - 20220103
IS  - 1432-1211 (Electronic)
IS  - 0093-7711 (Linking)
VI  - 72
IP  - 3
DP  - 2020 Apr
TI  - Development of a novel monoclonal antibody that binds to most HLA-A allomorphs in 
      a conformation-dependent yet peptide-promiscuous fashion.
PG  - 143-153
LID - 10.1007/s00251-020-01154-w [doi]
AB  - Specificity analyses of peptide binding to human leukocyte antigen (HLA)-A 
      molecules have been hampered due to a lack of proper monoclonal antibodies (mAbs) 
      for certain allomorphs, such as the prevalent HLA-A1 for Caucasians and HLA-A11 
      for Asians. We developed a mAb that recognizes a conformational epitope common to 
      most HLA-A allomorphs. The mAb, named A-1, does not discriminate peptides by 
      amino acid sequences, making it suitable for measuring peptide binding. A 
      stabilization assay using TAP-deficient cell lines and A-1 was developed to 
      investigate the specificity of peptide binding to HLA-A molecules. Regarding the 
      evolution of HLA-A genes, the A-1 epitope has been conserved among most HLA-A 
      allomorphs but was lost when the HLA-A gene diversified into the HLA-A*32, 
      HLA-A*31, and HLA-A*33 lineages together with HLA-A*29 after bifurcating from the 
      HLA-A*25 and HLA-A*26 branchs. The establishment of A-1 is expected to help 
      researchers investigate the peptide repertoire and develop computational tools to 
      identify cognate peptides. Since no HLA-A locus-specific mAb has been available, 
      A-1 will also be useful for analyzing the locus-specific regulation of the HLA 
      gene expression.
FAU - Komatsu, Toshihiro
AU  - Komatsu T
AD  - Department of Immunology, Kochi University, Kochi, Japan.
FAU - Shimizu, Takeyuki
AU  - Shimizu T
AD  - Department of Immunology, Kochi University, Kochi, Japan.
FAU - Kanoh, Makoto
AU  - Kanoh M
AD  - Department of Immunology, Kochi University, Kochi, Japan.
FAU - Miyakawa, Tomoya
AU  - Miyakawa T
AD  - Corporate Business Development Division, NEC, Tokyo, Japan.
FAU - Satta, Yoko
AU  - Satta Y
AD  - Department of Evolutionary Studies of Biosystems, SOKENDAI (The Graduate 
      University for Advanced Studies), Hayama, Japan.
FAU - Yasukochi, Yoshiki
AU  - Yasukochi Y
AD  - Department of Evolutionary Studies of Biosystems, SOKENDAI (The Graduate 
      University for Advanced Studies), Hayama, Japan.
AD  - Department of Human Functional Genomics, Advanced Science Research Promotion 
      Center, Mie University, Tsu, Japan.
FAU - Fujimoto, Rika
AU  - Fujimoto R
AD  - Department of Immunology, Kochi University, Kochi, Japan.
AD  - Research Institute at Kanagawa Cancer Centre, Yokohama, Japan.
FAU - Tada, Motoki
AU  - Tada M
AD  - Department of Immunology, Kochi University, Kochi, Japan.
AD  - Japan Manned Space Systems Corporation, Tokyo, Japan.
FAU - Machida, Kaori
AU  - Machida K
AD  - Department of Immunology, Kochi University, Kochi, Japan.
FAU - Kataoka, Sayo
AU  - Kataoka S
AD  - Science Research Center, School of Medicine, Kochi University, Kochi, Japan.
FAU - Udaka, Keiko
AU  - Udaka K
AD  - Department of Immunology, Kochi University, Kochi, Japan. udaka@kochi-u.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200122
PL  - United States
TA  - Immunogenetics
JT  - Immunogenetics
JID - 0420404
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Epitopes)
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-A1 Antigen)
RN  - 0 (Peptides)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antibodies, Monoclonal/immunology/*metabolism
MH  - Cell Line, Tumor
MH  - Epitopes/immunology
MH  - HLA-A Antigens/chemistry/*immunology
MH  - HLA-A1 Antigen/chemistry/*immunology
MH  - Humans
MH  - Models, Molecular
MH  - Peptides/immunology
MH  - Protein Binding/immunology
MH  - Protein Conformation
OTO - NOTNLM
OT  - Conformational epitope
OT  - HLA-A
OT  - Locus-specific
OT  - Monoclonal antibody
OT  - Peptide-promiscuous
EDAT- 2020/01/24 06:00
MHDA- 2020/05/12 06:00
CRDT- 2020/01/24 06:00
PHST- 2019/11/21 00:00 [received]
PHST- 2020/01/13 00:00 [accepted]
PHST- 2020/01/24 06:00 [pubmed]
PHST- 2020/05/12 06:00 [medline]
PHST- 2020/01/24 06:00 [entrez]
AID - 10.1007/s00251-020-01154-w [pii]
AID - 10.1007/s00251-020-01154-w [doi]
PST - ppublish
SO  - Immunogenetics. 2020 Apr;72(3):143-153. doi: 10.1007/s00251-020-01154-w. Epub 
      2020 Jan 22.