PMID- 31970677
OWN - NLM
STAT- MEDLINE
DCOM- 20210713
LR  - 20210713
IS  - 1993-0402 (Electronic)
IS  - 1672-0415 (Linking)
VI  - 26
IP  - 9
DP  - 2020 Sep
TI  - Inhibitory Mechanisms of Water Extract of Oplopanax elatus on 
      Lipopolysaccharide-Induced Inflammatory Responses in RAW 264.7 Murine Macrophage 
      Cells.
PG  - 670-676
LID - 10.1007/s11655-020-3188-2 [doi]
AB  - OBJECTIVE: To study the anti-inflammatory action and cellular mechanism of 
      Oplopanax elatus. METHODS: A hot water extract of OE (WOE) was prepared and a 
      major constituent, syringin, was successfully isolated. Its content in WOE was 
      found to be 214.0 microg/g dried plant (w/w). Their anti-inflammatory activities were 
      examined using RAW 264.7 macrophages and a mouse model of croton oil-induced ear 
      edema. RESULTS: In lipopolysaccharide (LPS)-treated RAW 264.7 cells, a mouse 
      macrophage cell line, WOE was found to significantly and strongly inhibit 
      cyclooxygenase-2 (COX-2)-induced prostaglandin E(2) (PGE(2)) production [half 
      maximal inhibitory concentration (IC(50))=135.2 microg/mL] and inducible nitric oxide 
      synthase (iNOS)-induced NO production (IC(50)=242.9 microg/mL). In the same 
      condition, WOE was revealed to inhibit NO production by down-regulating iNOS 
      expression, mainly by interrupting mitogen activated protein kinases 
      (MAPKs)/activator protein-1 (AP-1) pathway. The activation of all three major 
      MAPKs, p38 MAPK, extracellular signal-regulated kinase (ERK), and c-Jun 
      N-terminal kinase, was inhibited by WOE (50-300 microg/mL). On the other hand, WOE 
      reduced PGE(2) production by inhibiting COX-2 enzyme activity, but did not affect 
      COX-2 expression levels. In addition, WOE inhibited the production of 
      proinflammatory cytokines such as interleukin-6 and tumor necrosis factor-alpha. In 
      croton oil-induced ear edema in mice, oral administration of WOE (50-300 mg/kg) 
      dose-dependently inhibited edematic inflammation. CONCLUSION: Water extract of OE 
      exhibited multiple anti-inflammatory action mechanisms and may have potential for 
      treating inflammatory disorders.
FAU - Kwon, Ki Sun
AU  - Kwon KS
AD  - College of Pharmacy, Kangwon National University, Chuncheon, 24341, Republic of 
      Korea.
FAU - Lim, Hyun
AU  - Lim H
AD  - College of Pharmacy, Kangwon National University, Chuncheon, 24341, Republic of 
      Korea.
FAU - Kwon, Yong Soo
AU  - Kwon YS
AD  - College of Pharmacy, Kangwon National University, Chuncheon, 24341, Republic of 
      Korea.
FAU - Kim, Myong Jo
AU  - Kim MJ
AD  - Department of Bio-Resource Sciences, Kangwon National University, Chuncheon, 
      24341, Republic of Korea.
FAU - Yoo, Ji Hye
AU  - Yoo JH
AD  - Bioherb Research Institute, Kangwon National University, Chuncheon, 24341, 
      Republic of Korea.
FAU - Yoo, Nam Ho
AU  - Yoo NH
AD  - Department of Bio-Resource Sciences, Kangwon National University, Chuncheon, 
      24341, Republic of Korea.
FAU - Kim, Hyun Pyo
AU  - Kim HP
AD  - College of Pharmacy, Kangwon National University, Chuncheon, 24341, Republic of 
      Korea. hpkim@kangwon.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20200123
PL  - China
TA  - Chin J Integr Med
JT  - Chinese journal of integrative medicine
JID - 101181180
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Plant Extracts)
RN  - 059QF0KO0R (Water)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/chemistry/pharmacology
MH  - Inflammation/chemically induced/metabolism/*prevention & control
MH  - Inflammation Mediators/metabolism
MH  - Lipopolysaccharides
MH  - Macrophages/*drug effects/physiology
MH  - Mice
MH  - Oplopanax/*chemistry
MH  - Plant Extracts/chemistry/*pharmacology
MH  - RAW 264.7 Cells
MH  - Water/chemistry
OTO - NOTNLM
OT  - Chinese medicine
OT  - Oplopanax elatus
OT  - RAW 264.7
OT  - anti-inflammation
OT  - stem
OT  - syringin
EDAT- 2020/01/24 06:00
MHDA- 2021/07/14 06:00
CRDT- 2020/01/24 06:00
PHST- 2018/11/23 00:00 [accepted]
PHST- 2020/01/24 06:00 [pubmed]
PHST- 2021/07/14 06:00 [medline]
PHST- 2020/01/24 06:00 [entrez]
AID - 10.1007/s11655-020-3188-2 [pii]
AID - 10.1007/s11655-020-3188-2 [doi]
PST - ppublish
SO  - Chin J Integr Med. 2020 Sep;26(9):670-676. doi: 10.1007/s11655-020-3188-2. Epub 
      2020 Jan 23.