PMID- 31972488
OWN - NLM
STAT- MEDLINE
DCOM- 20200422
LR  - 20221207
IS  - 1879-1298 (Electronic)
IS  - 0045-6535 (Linking)
VI  - 247
DP  - 2020 May
TI  - Optimization of an enriched mixed culture to increase PHA accumulation using 
      industrial saline complex wastewater as a substrate.
PG  - 125873
LID - S0045-6535(20)30064-3 [pii]
LID - 10.1016/j.chemosphere.2020.125873 [doi]
AB  - Polyhydroxyalkanoates (PHA) appear as good candidates to substitute conventional 
      petroleum-based plastics since they have similar properties but with the 
      advantage of being biodegradable. Wastewater streams with high organic content 
      are feasible substrates for PHA production resulting in an opportunity for waste 
      recovery. One of the main challenges is the optimization of the selection of 
      microorganisms with high PHA storage capacity. This microbial selection is 
      performed in sequencing batch reactors (SBR) operated under an aerobic 
      feast/famine (F/F) regime. In the present study, a settling stage was added at 
      the end of the feast phase of the enrichment cycle of a SBR fed with 
      pre-acidified cooked mussel processing wastewater (containing up to 12 g NaCl/L). 
      Settling and subsequent supernatant discharge favoured the wash-out of 
      non-accumulating microorganisms as well as the removal of substances that 
      enhanced their undesired development (proteins and carbohydrates). Microbial 
      analysis performed by fluorescence in situ hybridization (FISH) technique showed 
      shifts in the microbial community; the presence of genus Paracoccus increased 
      whereas genera Comamonas decreased. Moreover, the process efficiency was improved 
      with the increase of the PHA production yield (Y(PHA)) and the maximum PHA 
      storage capacity (max. PHA) from 0.48 to 0.72 Cmmol(PHA)/Cmmol(VFA) and from 40 
      to 60 wt%, respectively. The polymer composition also changed, its HB:HV ratio 
      varied from 83:17 to 70:30. Results obtained in the present study showed that 
      settling after the feast phase promoted the removal of carbon sources that did 
      not contribute to PHA production and the washout of non-storing bacteria, which 
      favoured the culture enrichment.
CI  - Copyright (c) 2020 Elsevier Ltd. All rights reserved.
FAU - Argiz, Lucia
AU  - Argiz L
AD  - CRETUS Institute, Universidade de Santiago de Compostela, 15782, Santiago de 
      Compostela, Galicia, Spain. Electronic address: luciaargiz.montes@usc.es.
FAU - Fra-Vazquez, Andrea
AU  - Fra-Vazquez A
AD  - CRETUS Institute, Universidade de Santiago de Compostela, 15782, Santiago de 
      Compostela, Galicia, Spain.
FAU - Del Rio, Angeles Val
AU  - Del Rio AV
AD  - CRETUS Institute, Universidade de Santiago de Compostela, 15782, Santiago de 
      Compostela, Galicia, Spain.
FAU - Mosquera-Corral, Anuska
AU  - Mosquera-Corral A
AD  - CRETUS Institute, Universidade de Santiago de Compostela, 15782, Santiago de 
      Compostela, Galicia, Spain.
LA  - eng
PT  - Journal Article
DEP - 20200111
PL  - England
TA  - Chemosphere
JT  - Chemosphere
JID - 0320657
RN  - 0 (Carbohydrates)
RN  - 0 (Polyhydroxyalkanoates)
RN  - 0 (Waste Water)
RN  - 7440-44-0 (Carbon)
SB  - IM
MH  - Bacteria/classification
MH  - Bioreactors/microbiology
MH  - Carbohydrates
MH  - Carbon
MH  - In Situ Hybridization, Fluorescence
MH  - Polyhydroxyalkanoates/*chemistry
MH  - Waste Disposal, Fluid/*methods
MH  - Wastewater/*chemistry
OTO - NOTNLM
OT  - Bioplastics
OT  - Enrichment
OT  - Industrial wastewater
OT  - Mixed microbial culture
OT  - Polyhydroxyalkanoates
OT  - Valorization
EDAT- 2020/01/24 06:00
MHDA- 2020/04/23 06:00
CRDT- 2020/01/24 06:00
PHST- 2019/10/14 00:00 [received]
PHST- 2019/12/25 00:00 [revised]
PHST- 2020/01/06 00:00 [accepted]
PHST- 2020/01/24 06:00 [pubmed]
PHST- 2020/04/23 06:00 [medline]
PHST- 2020/01/24 06:00 [entrez]
AID - S0045-6535(20)30064-3 [pii]
AID - 10.1016/j.chemosphere.2020.125873 [doi]
PST - ppublish
SO  - Chemosphere. 2020 May;247:125873. doi: 10.1016/j.chemosphere.2020.125873. Epub 
      2020 Jan 11.