PMID- 31973723
OWN - NLM
STAT- MEDLINE
DCOM- 20200514
LR  - 20240328
IS  - 1475-2859 (Electronic)
IS  - 1475-2859 (Linking)
VI  - 19
IP  - 1
DP  - 2020 Jan 23
TI  - Beneficial factors for biomineralization by ureolytic bacterium Sporosarcina 
      pasteurii.
PG  - 12
LID - 10.1186/s12934-020-1281-z [doi]
LID - 12
AB  - BACKGROUND: The ureolytic bacterium Sporosarcina pasteurii is well-known for its 
      capability of microbially induced calcite precipitation (MICP), representing a 
      great potential in constructional engineering and material applications. However, 
      the molecular mechanism for its biomineralization remains unresolved, as few 
      studies were carried out. RESULTS: The addition of urea into the culture medium 
      provided an alkaline environment that is suitable for S. pasteurii. As compared 
      to S. pasteurii cultivated without urea, S. pasteurii grown with urea showed 
      faster growth and urease production, better shape, more negative surface charge 
      and higher biomineralization ability. To survive the unfavorable growth 
      environment due to the absence of urea, S. pasteurii up-regulated the expression 
      of genes involved in urease production, ATPase synthesis and flagella, possibly 
      occupying resources that can be deployed for MICP. As compared to 
      non-mineralizing bacteria, S. pasteurii exhibited more negative cell surface 
      charge for binding calcium ions and more robust cell structure as nucleation 
      sites. During MICP process, the genes for ATPase synthesis in S. pasteurii was 
      up-regulated while genes for urease production were unchanged. Interestingly, 
      genes involved in flagella were down-regulated during MICP, which might lead to 
      poor mobility of S. pasteurii. Meanwhile, genes in fatty acid degradation pathway 
      were inhibited to maintain the intact cell structure found in calcite 
      precipitation. Both weak mobility and intact cell structure are advantageous for 
      S. pasteurii to serve as nucleation sites during MICP. CONCLUSIONS: Four factors 
      are demonstrated to benefit the super performance of S. pasteurii in MICP. First, 
      the good correlation of biomass growth and urease production of S. pasteurii 
      provides sufficient biomass and urease simultaneously for improved 
      biomineralization. Second, the highly negative cell surface charge of S. 
      pasteurii is good for binding calcium ions. Third, the robust cell structure and 
      fourth, the weak mobility, are key for S. pasteurii to be nucleation sites during 
      MICP.
FAU - Ma, Liang
AU  - Ma L
AD  - State Key Laboratory of Bioelectronics, School of Biological Science and Medical 
      Engineering, Southeast University, Nanjing, 210096, Jiangsu, People's Republic of 
      China.
FAU - Pang, Ai-Ping
AU  - Pang AP
AD  - State Key Laboratory of Bioelectronics, School of Biological Science and Medical 
      Engineering, Southeast University, Nanjing, 210096, Jiangsu, People's Republic of 
      China.
FAU - Luo, Yongsheng
AU  - Luo Y
AD  - State Key Laboratory of Bioelectronics, School of Biological Science and Medical 
      Engineering, Southeast University, Nanjing, 210096, Jiangsu, People's Republic of 
      China.
FAU - Lu, Xiaolin
AU  - Lu X
AD  - State Key Laboratory of Bioelectronics, School of Biological Science and Medical 
      Engineering, Southeast University, Nanjing, 210096, Jiangsu, People's Republic of 
      China. lxl@seu.edu.cn.
FAU - Lin, Fengming
AU  - Lin F
AUID- ORCID: 0000-0002-6211-1916
AD  - State Key Laboratory of Bioelectronics, School of Biological Science and Medical 
      Engineering, Southeast University, Nanjing, 210096, Jiangsu, People's Republic of 
      China. linfengming@seu.edu.cn.
LA  - eng
GR  - 31700040/National Natural Science Foundation of China/
GR  - 2242019K3DN01/Fundamental Research Funds for the Central Universities/
PT  - Journal Article
DEP - 20200123
PL  - England
TA  - Microb Cell Fact
JT  - Microbial cell factories
JID - 101139812
RN  - 0 (Culture Media)
RN  - 8W8T17847W (Urea)
RN  - EC 2.7.4.- (ATP Synthetase Complexes)
RN  - EC 3.5.1.5 (Urease)
RN  - H0G9379FGK (Calcium Carbonate)
RN  - Sporosarcina pasteurii
SB  - IM
MH  - ATP Synthetase Complexes/*metabolism
MH  - Biomineralization/*physiology
MH  - Calcium Carbonate/*metabolism
MH  - Culture Media/chemistry
MH  - Gene Expression Profiling
MH  - Genome, Bacterial
MH  - Microscopy, Electron, Scanning
MH  - *Sporosarcina/genetics/metabolism/ultrastructure
MH  - Urea
MH  - Urease/*genetics
PMC - PMC6979283
OTO - NOTNLM
OT  - ATP synthase
OT  - Bacterial surface potential
OT  - Biomineralization
OT  - Sporosarcina pasteurii
OT  - Urease
COIS- The authors declare that they have no competing interests.
EDAT- 2020/01/25 06:00
MHDA- 2020/05/15 06:00
PMCR- 2020/01/23
CRDT- 2020/01/25 06:00
PHST- 2019/10/30 00:00 [received]
PHST- 2020/01/09 00:00 [accepted]
PHST- 2020/01/25 06:00 [entrez]
PHST- 2020/01/25 06:00 [pubmed]
PHST- 2020/05/15 06:00 [medline]
PHST- 2020/01/23 00:00 [pmc-release]
AID - 10.1186/s12934-020-1281-z [pii]
AID - 1281 [pii]
AID - 10.1186/s12934-020-1281-z [doi]
PST - epublish
SO  - Microb Cell Fact. 2020 Jan 23;19(1):12. doi: 10.1186/s12934-020-1281-z.