PMID- 31973852
OWN - NLM
STAT- MEDLINE
DCOM- 20200221
LR  - 20231213
IS  - 1095-9939 (Electronic)
IS  - 0048-3575 (Linking)
VI  - 163
DP  - 2020 Feb
TI  - Effects of epsilon-poly-l-lysine on vegetative growth, pathogenicity and gene 
      expression of Alternaria alternata infecting Nicotiana tabacum.
PG  - 147-153
LID - S0048-3575(19)30488-2 [pii]
LID - 10.1016/j.pestbp.2019.11.005 [doi]
AB  - Microbial secondary metabolites produced by Streptomyces are applied to control 
      plant diseases. epsilon-poly-l-lysine (epsilon-PL) is a non-toxic food preservative, but the 
      potential application of epsilon-PL as a microbial fungicide in agriculture has rarely 
      been reported. In this study, Alternaria alternata (A. alternata) was used to 
      reveal the effect and mode of action for epsilon-PL on the plant pathogenic fungi. The 
      results showed that epsilon-PL effectively inhibited necrotic-lesion development caused 
      by A. alternata on tobacco. Mycelial growth was also significantly inhibited in 
      vitro by 100 mug/ml epsilon-PL using in vitro analysis. Moreover, 25 mug/ml epsilon-PL 
      inhibited spore germination and induced abnormal morphological development of A. 
      alternata hyphae. To clarify the molecular-genetic antifungal mechanisms, we 
      selected several crucial genes involved in the development and pathogenesis of A. 
      alternata and studied their expression regulated by epsilon-PL. Results of real-time 
      quantitative PCR showed that a mycelium morphology and pathogenic process related 
      cyclic adenosine monophosphate protein (cAMP) dependent protein kinase A (PKA), 
      Alternaria alternata cAMP-dependent protein kinase catalytic subunit (AAPK1) and 
      the early infection-related glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were 
      down-regulated after epsilon-PL treatment. The results provide novel insights for the 
      application of epsilon-PL in the control of plant diseases caused by A. alternata.
CI  - Copyright (c) 2019 Elsevier Inc. All rights reserved.
FAU - Liu, He
AU  - Liu H
AD  - College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, 
      Liaoning, China.
FAU - Chen, Jianguang
AU  - Chen J
AD  - College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, 
      Liaoning, China.
FAU - Xia, Zihao
AU  - Xia Z
AD  - College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, 
      Liaoning, China.
FAU - An, Mengnan
AU  - An M
AD  - College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, 
      Liaoning, China. Electronic address: anmengnan1984@163.com.
FAU - Wu, Yuanhua
AU  - Wu Y
AD  - College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, 
      Liaoning, China. Electronic address: wuyh09@syau.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20191111
PL  - United States
TA  - Pestic Biochem Physiol
JT  - Pesticide biochemistry and physiology
JID - 1301573
RN  - 25104-18-1 (Polylysine)
SB  - IM
MH  - *Alternaria
MH  - Plant Diseases
MH  - Polylysine
MH  - *Nicotiana
MH  - Virulence
OTO - NOTNLM
OT  - Alternaria alternata
OT  - Antifungal mechanisms
OT  - Nicotiana tobacum
OT  - epsilon-Poly-l-lysine
COIS- Declaration of Competing Interest The authors declare no competing financial 
      interest.
EDAT- 2020/01/25 06:00
MHDA- 2020/02/23 06:00
CRDT- 2020/01/25 06:00
PHST- 2019/06/23 00:00 [received]
PHST- 2019/11/04 00:00 [revised]
PHST- 2019/11/06 00:00 [accepted]
PHST- 2020/01/25 06:00 [entrez]
PHST- 2020/01/25 06:00 [pubmed]
PHST- 2020/02/23 06:00 [medline]
AID - S0048-3575(19)30488-2 [pii]
AID - 10.1016/j.pestbp.2019.11.005 [doi]
PST - ppublish
SO  - Pestic Biochem Physiol. 2020 Feb;163:147-153. doi: 10.1016/j.pestbp.2019.11.005. 
      Epub 2019 Nov 11.